Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Assay Kit / ADA/PK Assay Elisa Kit

Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (H3L)

Catalog #:   KAV12701 Specific References (104) DATASHEET
Applications: Used for the quantitative determination of Human Anti-Monkeypox virus IgG (H3L) concentration in serum and plasma.
Sample type: Plasma, Serum
Sensitivity: 235.00 ng/mL
Range: 468.75 - 30,000 ng/mL
Overview

Catalog No.

KAV12701

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus H3L antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (H3L) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (H3L). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (H3L) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

H3L

Applications

Used for the quantitative determination of Human Anti-Monkeypox virus IgG (H3L) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

468.75 - 30,000 ng/mL

Sensitivity

235.00 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

 

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

16

16

16

24

24

24

Mean (ng/mL)

14016.4

3933.7

1003.5

14800.0

3916.3

927.8

Standard deviation

1272.4

372.6

103.8

1197.0

417.3

93.1

CV (%)

9.1

9.5

10.3

8.1

10.7

10.0

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Data Image
  • Experiment Example
    CALCULATION OF RESULTS
    Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. Do not include the blank in the standard curve. The data may be linearized by plotting the log of the Human Anti-Monkeypox virus IgG (H3L) concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
References

New p35 (H3L) Epitope Involved in Vaccinia Virus Neutralization and Its Deimmunization. PMID: 35746695

Vaccinia virus H3L envelope protein is a major target of neutralizing antibodies in humans and elicits protection against lethal challenge in mice. PMID: 16140750

Poly[bis-[μ(2)-(dimethyl-aza-nium-yl)methyl-enediphospho-nato]magnesium]. PMID: 21522287

Vaccinia virus envelope H3L protein binds to cell surface heparan sulfate and is important for intracellular mature virion morphogenesis and virus infection in vitro and in vivo. PMID: 10708453

Cell population data: much more to explore. PMID: 36622868

Characterization of the vaccinia virus H3L envelope protein: topology and posttranslational membrane insertion via the C-terminal hydrophobic tail. PMID: 10906204

Effects of deletion or stringent repression of the H3L envelope gene on vaccinia virus replication. PMID: 10906205

Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens. PMID: 36687315

Molecular Modelling and Insilico Engineering of PapMV-CP Towards Display and Development of Capripox Viral Like Particles Based on Immunogenic P32 Envelop Protein is the Homologous of the Vaccinia-Viral H3L Gene: An Insilico Approach. PMID: 32421016

Phylogenetic analysis of three genes of Penguinpox virus corresponding to Vaccinia virus G8R (VLTF-1), A3L (P4b) and H3L reveals that it is most closely related to Turkeypox virus, Ostrichpox virus and Pigeonpox virus. PMID: 19426497

Synthesis, structure, and phosphatase-like activity of a new trinuclear Gd complex with the unsymmetrical ligand H3L as a model for nucleases. PMID: 20170188

High spin d5 complexes of tris(6-hydroxymethyl-2-pyridylmethyl)amine (H3L): hepta-coordinated [Mn(H3L)]Cl2 and linear trinuclear [Fe3L2](ClO4)3. PMID: 18185873

4-. PMID: 10508341

Experimental charge-density study on the nickel(II) coordination complex [Ni(H3L)][NO3][PF6] [H3L = N,N',N''-tris(2-hydroxy-3-methylbutyl)-1,4,7-triazacyclononane]: a reappraisal. PMID: 16552157

Hexaaqua-manganese(II) bis-{[N-(3-meth-oxy-2-oxidobenzyl-idene)glycylglycinato]copper(II)} hexa-hydrate. PMID: 21579016

Evolution of the orthopoxvirus core genome. PMID: 36280003

A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. PMID: 10485266

A variety of spin-crossover behaviors depending on the counter anion: two-dimensional complexes constructed by NH...Cl- hydrogen bonds, [FeIIH3LMe]Cl.X (X = PF6 -, AsF6 -, SbF6 -, CF3SO3 -; H3L(Me) = Tris[2-{[(2-methylimidazol-4-yl)methylidene]amino}ethyl]amine). PMID: 16575927

Binding and removal of sulfate, phosphate, arsenate, tetrachloromercurate, and chromate in aqueous solution by means of an activated carbon functionalized with a pyrimidine-based anion receptor (HL). Crystal structures of [H3L(HgCl4)]·H2O and [H3L(HgBr4)]·H2O showing anion-π interactions. PMID: 20836504

Nature of copper(II)-lanthanide(III) magnetic interactions and generation of a large magnetic moment with magnetic anisotropy of 3d-4f cyclic cylindrical tetranuclear complexes [CuIILLnIII(hfac)2]2, (H3L = 1-(2-hydroxybenzamido)-2-(2-hydroxy-3-methoxybenzylideneamino)ethane and Hhfac = hexafluoroacetylacetone, LnIII = Eu, Gd, Tb, Dy). PMID: 12693220

Observing an analyzer's operational life cycle: a useful management tool for clinical laboratories. PMID: 30099409

Tunable Optical Molecular Thermometers Based on Metallacrowns. PMID: 36173924

Lanthanide cryptate monometallic coordination complexes. PMID: 32955062

Synthesis, characterization and antitubercular activities of heterocyclic selenosemicarbazones. PMID: 35661528

A new family of "H3L-like" histone genes. PMID: 15638457

Interlayer interaction of two-dimensional layered spin crossover complexes [FeIIH3L(Me)][FeIIL(Me)]X (X-=ClO4-, BF4-, PF6-, AsF6-, and SbF6-; H3L(Me)=tris[2-(((2-methylimidazol-4-yl)methylidene)amino)ethyl]amine). PMID: 14658894

Electronic structures and selective fluoride sensing features of Os(bpy)2(HL(2-)) and [{Os(bpy)2}2(μ-HL(2-))](2+) (H3L: 5-(1H-benzo[d]imidazol-2-yl)-1H-imidazole-4-carboxylic acid). PMID: 25112369

Design of pure heterodinuclear lanthanoid cryptate complexes. PMID: 34123326

ELISA for Molluscum Contagiosum Virus. PMID: 29120484

Antibacterial Activity of Two Zn-MOFs Containing a Tricarboxylate Linker. PMID: 36500760

Basophil counting in hematology analyzers: time to discontinue? PMID: 33554563

Isolation of a (dinitrogen)tricopper(I) complex. PMID: 25238198

Single-Molecule-Magnet FeII4FeIII2 and Antiferromagnetic FeIII4 Coordination Clusters. PMID: 31136160

A self-healable metallohydrogel for drug encapsulations and drug release. PMID: 37223407

Stepwise Coordination Assembly Approach toward Aluminum-Lanthanide-based Compounds. PMID: 32883079

Synthesis and magnetic properties of heterometal cyclic tetranuclear complexes [Cu(II)LM(II)(hfac)]2 (M(II) = Zn, Cu, Ni, Co, Fe, Mn; H3L = 1-(2-hydroxybenzamido)-2-((2-hydroxy-3-methoxybenzylidene)amino)ethane; Hhfac = hexafluoroacetylacetone). PMID: 12950197

An unusual open cubane structure in a mu(1,1)-azido- and alkoxo-bridged tetranuclear copper(II) complex, [Cu4L2(mu(1,1)-N3)2].5H2O (H3L = N,N'-(2-Hydroxylpropane-1,3-diyl)bis-salicylideneimine). PMID: 15476386

Solvent-Vapor-Induced Reversible Single-Crystal-to-Single-Crystal Transformation of a Triphosphaazatriangulene-Based Metal-Organic Framework. PMID: 31773880

Zn(II) coordination polymers: therapeutic activity and nursing application values against coronary heart disease. PMID: 35711619

Template Synthesis of Three-Dimensional Hexakisimidazolium Cages. PMID: 29394472

Biotin-conjugated tumour-targeting photocytotoxic iron(III) complexes. PMID: 23776297

Probing and pressing surfaces of hepatitis C virus-like particles. PMID: 30897421

Different Phenomena in Magnetic/Electrical Properties of Co(II) and Ni(II) Isomorphous MOFs. PMID: 33842790

Three-in-One Structural-Building-Mode-Based Ti16-Type Titanium Oxo Cluster Entirely Protected by the Ligands Benzoate and Salicylhydroxamate. PMID: 35639458

Two Mn(II) and Zn(II) Coordination Polymers: Aqueous-Phase Detection of Nitroaromatic Explosives and Protective Effect on Atherosclerosis. PMID: 35015178

Crystal transformation in Mn(II) metal-organic frameworks based on a one-dimensional chain precursor. PMID: 34152335

Dodecanuclear [Cu(II)6Gd(III)6] nanoclusters as magnetic refrigerants. PMID: 22409581

Modulating the Inclusive and Coordinating Ability of Thiacalix[4]arene and Its Antenna Effect on Yb3-Luminescence via Upper-Rim Substitution. PMID: 36296384

Self-Assembly of Antiferromagnetically-Coupled Copper(II) Supramolecular Architectures with Diverse Structural Complexities. PMID: 33256163

Charge Neutral [Cu2L2] and [Pd2L2] Metallocycles: Self-Assembly, Aggregation, and Catalysis. PMID: 34114797

Metal-Ion Metathesis and Properties of Triarylboron-Functionalized Metal-Organic Frameworks. PMID: 25929722

From Hydrogen Bond to van der Waals Force: Molecular Scalpel Strategy to Exfoliate a Two-Dimensional Metal-Organic Nanosheet. PMID: 35354284

Four new lanthanide-organic frameworks: selective luminescent sensing and magnetic properties. PMID: 27464998

Vanadium complexes with multidentate amine bisphenols. PMID: 25117739

Immunogenicity of MVA-BN vaccine deployed as mpox prophylaxis: a prospective, single-centre, cohort study and analysis of transcriptomic predictors of response., PMID:40286799

The two-dose MVA-BN mpox vaccine induces a nondurable and low avidity MPXV-specific antibody response., PMID:40162783

Rational mpox vaccine design: immunogenicity and protective effect of individual and multicomponent proteins in mice., PMID:40105863

Development and validation of a quantitative Orthopoxvirus immunoassay to evaluate and differentiate serological responses to Mpox infection and vaccination., PMID:39987746

Short-term evolution of Mpox-specific IgG and neutralizing antibodies among individuals undergoing MVA-BN vaccination., PMID:39894441

Enhanced Immunogenicity and Affinity with A35R-Fc-Based Chimeric Protein Compared to MPXV A35R Protein., PMID:39861905

Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study., PMID:39832516

Short-Lived Neutralizing Antibody Responses to Monkeypox Virus in Smallpox Vaccine-Naive Persons after JYNNEOS Vaccination., PMID:39793541

An mpox quadrivalent mRNA vaccine elicits sustained and protective immunity in mice against lethal vaccinia virus challenge., PMID:39745170

Long-Lasting Protection and Dose Optimization of MPXV Polyvalent Mpox mRNA Vaccines Against Lethal Vaccinia Virus Challenge in Mice., PMID:39726255

Elderly Individuals Exhibit Elevated Levels of Anti-Monkeypox Virus Antibodies Compared to Adults, Youth, and Children., PMID:39569454

Unique Kinetics of the Human Milk Antibody Response to JYNNEOS Vaccine for Prevention of Monkey Pox: A Case Study., PMID:39360771

Rational design and computational evaluation of a multi-epitope vaccine for monkeypox virus: Insights into binding stability and immunological memory., PMID:39247273

Investigating seroprevalence of IgG antibodies against Monkeypox Virus (MPXV) in a cohort of people living with HIV (PLWH) in Rome, during the 2022 outbreak: Moving beyond traditional at-risk populations., PMID:39241937

An Attenuated and Highly Immunogenic Variant of the Vaccinia Virus., PMID:39188266

Profiling of viral load, antibody and inflammatory response of people with monkeypox during hospitalization: a prospective longitudinal cohort study in China., PMID:39043012

Clinical characteristics, viral dynamics, and antibody response of monkeypox virus infections among men with and without HIV infection in Guangzhou, China., PMID:38979508

Trimerized S expressed by modified vaccinia virus Ankara (MVA) confers superior protection against lethal SARS-CoV-2 challenge in mice., PMID:38874361

Immune responses associated with mpox viral clearance in men with and without HIV in Spain: a multisite, observational, prospective cohort study., PMID:38857615

Third-generation smallpox vaccines induce low-level cross-protecting neutralizing antibodies against Monkeypox virus in laboratory workers., PMID:38826712

Longitudinal viral shedding and antibody response characteristics of men with acute infection of monkeypox virus: a prospective cohort study., PMID:38802350

Antibodies Induced by Smallpox Vaccination after at Least 45 Years Cross-React with and In Vitro Neutralize Mpox Virus: A Role for Polyclonal B Cell Activation?, PMID:38675961

Scenarios of future mpox outbreaks among men who have sex with men: a modelling study based on cross-sectional seroprevalence data from the Netherlands, 2022., PMID:38666400

Mpox outbreak in Rio de Janeiro, Brazil: A translational approach., PMID:38654686

Breakthrough Mpox Outbreak Investigation, the Delicate Balance Between Host Immune Response and Viral Immune Escape., PMID:38647249

A multivalent mRNA monkeypox virus vaccine (BNT166) protects mice and macaques from orthopoxvirus disease., PMID:38366591

Characterizing the acute antibody response of monkeypox and MVA-BN vaccine following an Australian outbreak., PMID:38240403

Review of virological methods for laboratory diagnosis and characterization of monkeypox virus (MPXV): lessons learned from the 2022 Mpox outbreak., PMID:38179904

Serological methods for the detection of antibodies against monkeypox virus applicable for laboratories with different biosafety levels., PMID:38054557

Serosurvey of Immunity to Monkeypox (Mpox) Virus Antigens in People Living with HIV in South Florida., PMID:38003819

Smallpox vaccination in a mouse model., PMID:37965374

Prevalence of Undiagnosed Monkeypox Virus Infections during Global Mpox Outbreak, United States, June-September 2022., PMID:37832516

Mpox as AIDS-defining event with a severe and protracted course: clinical, immunological, and virological implications., PMID:37778364

Duration of humoral immunity from smallpox vaccination and its cross-reaction with Mpox virus., PMID:37709783

A Monoclonal Antibody Produced in Glycoengineered Plants Potently Neutralizes Monkeypox Virus., PMID:37514995

Mpox virus mRNA-lipid nanoparticle vaccine candidates evoke antibody responses and drive protection against the Vaccinia virus challenge in mice., PMID:37429529

Cross-reactive antibodies against monkeypox virus exist in the population immunized with vaccinia Tian Tan strain in China., PMID:37392823

Early evaluation of the safety, reactogenicity, and immune response after a single dose of modified vaccinia Ankara-Bavaria Nordic vaccine against mpox in children: a national outbreak response., PMID:37336224

Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs., PMID:37317160

Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity., PMID:37300753

Profiling the acute phase antibody response against mpox virus in patients infected during the 2022 outbreak., PMID:37287343

Computational Vaccine Design for Poxviridae Family Viruses., PMID:37258933

Serological responses to smallpox A33 antigen and monkeypox A35 antigen in healthy people and people living with HIV-1 from Guangzhou, China., PMID:37212313

Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus., PMID:37117161

Proteomics-based vaccine targets annotation and design of subunit and mRNA-based vaccines for Monkeypox virus (MPXV) against the recent outbreak., PMID:37116237

Monkeypox (mpox) in immunosuppressed patients., PMID:37089133

Clinical characterization and placental pathology of mpox infection in hospitalized patients in the Democratic Republic of the Congo., PMID:37079637

Design and Optimization of a Monkeypox virus Specific Serological Assay., PMID:36986317

Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection., PMID:36748085

Comparison of the Effectiveness of Transepidemal and Intradermal Immunization of Mice with the Vacinia Virus., PMID:36694907

Datasheet
$ 1128
Product specifications
96 T 1128

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (H3L) [KAV12701]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only