Immunohistochemistry (IHC): Experimental Protocols and Troubleshooting Guide
Immunohistochemistry (IHC) is a technique that utilizes the principle of specific binding between antigens and antibodies in immunology. It identifies antigens (such as polypeptides and proteins) in tissue cells through chemical reactions that cause color-developing agents (fluorescein, enzymes, metal ions, isotopes) labeled on antibodies to develop color, enabling their localization, qualitative, and quantitative study. Due to its strong specificity, high sensitivity, and rapid and simple operation, immunofluorescence technology is widely applied in clinical pathological diagnosis and testing.
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