Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (E8L)

Catalog No.

KAV13201

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus E8L antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (E8L) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (E8L). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (E8L) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

E8L

Applications

Used for the quantitative determination of Human Anti-Monkeypox virus IgG (E8L) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

15.63 - 1,000 ng/mL

Sensitivity

8.10 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	368.8	112.2	28.6	348.8	95.7	24.9
Standard deviation	21.2	6.8	3.9	41.0	12.1	3.7
CV (%)	5.7	6.1	13.6	11.8	12.7	14.8

 

Recovery

80-110%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

A Vaccine Strategy Based on the Identification of an Annular Ganglioside Binding Motif in Monkeypox Virus Protein E8L. PMID: 36423140

Expression, purification and characterization of C2 domain of milk fat globule-EGF-factor 8-L. PMID: 17049264

Rational development of multicomponent mRNA vaccine candidates against mpox. PMID: 36947428

MFG-E8 in the retina and retinal pigment epithelium of rat and mouse. PMID: 17167398

Inhibition of monkeypox virus replication by RNA interference. PMID: 19889227

In silico designing a novel TLR4-mediating multiepitope vaccine against monkeypox via advanced immunoinformatics and bioinformatics approaches. PMID: 37129119

Design peptide and multi-epitope protein vaccine candidates against monkeypox virus using reverse vaccinology approach: an in-silico study. PMID: 37154825

Immunogenicity of MVA-BN vaccine deployed as mpox prophylaxis: a prospective, single-centre, cohort study and analysis of transcriptomic predictors of response., PMID:40286799

The two-dose MVA-BN mpox vaccine induces a nondurable and low avidity MPXV-specific antibody response., PMID:40162783

Rational mpox vaccine design: immunogenicity and protective effect of individual and multicomponent proteins in mice., PMID:40105863

Development and validation of a quantitative Orthopoxvirus immunoassay to evaluate and differentiate serological responses to Mpox infection and vaccination., PMID:39987746

Short-term evolution of Mpox-specific IgG and neutralizing antibodies among individuals undergoing MVA-BN vaccination., PMID:39894441

Enhanced Immunogenicity and Affinity with A35R-Fc-Based Chimeric Protein Compared to MPXV A35R Protein., PMID:39861905

Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study., PMID:39832516

Short-Lived Neutralizing Antibody Responses to Monkeypox Virus in Smallpox Vaccine-Naive Persons after JYNNEOS Vaccination., PMID:39793541

An mpox quadrivalent mRNA vaccine elicits sustained and protective immunity in mice against lethal vaccinia virus challenge., PMID:39745170

Long-Lasting Protection and Dose Optimization of MPXV Polyvalent Mpox mRNA Vaccines Against Lethal Vaccinia Virus Challenge in Mice., PMID:39726255

Elderly Individuals Exhibit Elevated Levels of Anti-Monkeypox Virus Antibodies Compared to Adults, Youth, and Children., PMID:39569454

Unique Kinetics of the Human Milk Antibody Response to JYNNEOS Vaccine for Prevention of Monkey Pox: A Case Study., PMID:39360771

Rational design and computational evaluation of a multi-epitope vaccine for monkeypox virus: Insights into binding stability and immunological memory., PMID:39247273

Investigating seroprevalence of IgG antibodies against Monkeypox Virus (MPXV) in a cohort of people living with HIV (PLWH) in Rome, during the 2022 outbreak: Moving beyond traditional at-risk populations., PMID:39241937

An Attenuated and Highly Immunogenic Variant of the Vaccinia Virus., PMID:39188266

Profiling of viral load, antibody and inflammatory response of people with monkeypox during hospitalization: a prospective longitudinal cohort study in China., PMID:39043012

Clinical characteristics, viral dynamics, and antibody response of monkeypox virus infections among men with and without HIV infection in Guangzhou, China., PMID:38979508

Trimerized S expressed by modified vaccinia virus Ankara (MVA) confers superior protection against lethal SARS-CoV-2 challenge in mice., PMID:38874361

Immune responses associated with mpox viral clearance in men with and without HIV in Spain: a multisite, observational, prospective cohort study., PMID:38857615

Third-generation smallpox vaccines induce low-level cross-protecting neutralizing antibodies against Monkeypox virus in laboratory workers., PMID:38826712

Longitudinal viral shedding and antibody response characteristics of men with acute infection of monkeypox virus: a prospective cohort study., PMID:38802350

Antibodies Induced by Smallpox Vaccination after at Least 45 Years Cross-React with and In Vitro Neutralize Mpox Virus: A Role for Polyclonal B Cell Activation?, PMID:38675961

Scenarios of future mpox outbreaks among men who have sex with men: a modelling study based on cross-sectional seroprevalence data from the Netherlands, 2022., PMID:38666400

Mpox outbreak in Rio de Janeiro, Brazil: A translational approach., PMID:38654686

Breakthrough Mpox Outbreak Investigation, the Delicate Balance Between Host Immune Response and Viral Immune Escape., PMID:38647249

A multivalent mRNA monkeypox virus vaccine (BNT166) protects mice and macaques from orthopoxvirus disease., PMID:38366591

Characterizing the acute antibody response of monkeypox and MVA-BN vaccine following an Australian outbreak., PMID:38240403

Review of virological methods for laboratory diagnosis and characterization of monkeypox virus (MPXV): lessons learned from the 2022 Mpox outbreak., PMID:38179904

Serological methods for the detection of antibodies against monkeypox virus applicable for laboratories with different biosafety levels., PMID:38054557

Serosurvey of Immunity to Monkeypox (Mpox) Virus Antigens in People Living with HIV in South Florida., PMID:38003819

Smallpox vaccination in a mouse model., PMID:37965374

Prevalence of Undiagnosed Monkeypox Virus Infections during Global Mpox Outbreak, United States, June-September 2022., PMID:37832516

Mpox as AIDS-defining event with a severe and protracted course: clinical, immunological, and virological implications., PMID:37778364

Duration of humoral immunity from smallpox vaccination and its cross-reaction with Mpox virus., PMID:37709783

A Monoclonal Antibody Produced in Glycoengineered Plants Potently Neutralizes Monkeypox Virus., PMID:37514995

Mpox virus mRNA-lipid nanoparticle vaccine candidates evoke antibody responses and drive protection against the Vaccinia virus challenge in mice., PMID:37429529

Cross-reactive antibodies against monkeypox virus exist in the population immunized with vaccinia Tian Tan strain in China., PMID:37392823

Early evaluation of the safety, reactogenicity, and immune response after a single dose of modified vaccinia Ankara-Bavaria Nordic vaccine against mpox in children: a national outbreak response., PMID:37336224

Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs., PMID:37317160

Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity., PMID:37300753

Profiling the acute phase antibody response against mpox virus in patients infected during the 2022 outbreak., PMID:37287343

Computational Vaccine Design for Poxviridae Family Viruses., PMID:37258933

Serological responses to smallpox A33 antigen and monkeypox A35 antigen in healthy people and people living with HIV-1 from Guangzhou, China., PMID:37212313

Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus., PMID:37117161

Proteomics-based vaccine targets annotation and design of subunit and mRNA-based vaccines for Monkeypox virus (MPXV) against the recent outbreak., PMID:37116237

Monkeypox (mpox) in immunosuppressed patients., PMID:37089133

Clinical characterization and placental pathology of mpox infection in hospitalized patients in the Democratic Republic of the Congo., PMID:37079637

Design and Optimization of a Monkeypox virus Specific Serological Assay., PMID:36986317

Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection., PMID:36748085

Comparison of the Effectiveness of Transepidemal and Intradermal Immunization of Mice with the Vacinia Virus., PMID:36694907

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