Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (A29L) (KAV13401)

Catalog No.

KAV13401

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus A29L antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (A29L) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (A29L). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (A29L) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

A29L

Applications

Used for the quantitative determination of Human Anti-Monkeypox virus IgG (A29L) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

469 ng/mL - 30,000 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	16282.8	4176.8	1072.5	16037.3	4253.0	1036.4
Standard deviation	1668.9	471.1	143.4	1661.9	466.5	213.8
CV (%)	10.2	11.3	13.4	10.4	11.0	20.6

Recovery

75-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Mpox vaccine and infection-driven human immune signatures. PMID: 36945651

Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus. PMID: 37288876

Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus. PMID: 37117161

Rational development of multicomponent mRNA vaccine candidates against mpox. PMID: 36947428

Molecular detection and analysis of Sheeppox and Orf viruses isolated from sheep from Qalubia, Egypt. PMID: 27529993

Design peptide and multi-epitope protein vaccine candidates against monkeypox virus using reverse vaccinology approach: an in-silico study. PMID: 37154825

BoHV-4-Based Vector Single Heterologous Antigen Delivery Protects STAT1(-/-) Mice from Monkeypoxvirus Lethal Challenge. PMID: 26086739

A duplex PCR assay for simultaneous detection and differentiation of Capripoxvirus and Orf virus. PMID: 17350223

Zeng, Y., Liu, X., Li, Y., Lu, J., Wu, Q., Dan, D., Lv, S., Xia, F., Hu, C., and Li, J. (2023). The assessment on cross immunity with smallpox virus and antiviral drug sensitivity of the isolated mpox virus strain WIBP-MPXV-001 in China. Emerging Microbes & Infections 12, 2208682. PMID: 37128898

Document Download

Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (A29L).pdf