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Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339) (RHF37501)

Host species:Mouse
Isotype:IgG2a, kappa
Applications:ELISA, WB
Accession:P60709
Overview

Catalog No.

RHF37501

Description

Loading control in ELISA,WB

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Tested applications

ELISA: 1:2000-1:4000, WB: 1:1000-1:4000

Target

Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P60709

Applications

ELISA, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0339

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with ACTB/β-actin/Beta Actin antibody (RHF37501) at 0.2 μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate
    Lane 4: PC12 cell lysate
    Lane 5: BV2 cell lysate

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 42 kDa
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with ACTB/β-actin/Beta Actin antibody (RHF37501) at 0.2 μg/ml.

    Lane 1: mouse kidney lysate
    Lane 2: mouse lung lysate
    Lane 3: rat kidney lysate
    Lane 4: rat lung lysate

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 42 kDa
References
Datasheet
$ 130
Product specifications
50 μg 130 100 μg 220

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339) [RHF37501]
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