Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Recombinant Antibody

Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339)

Catalog #:   RHF37501 Specific References (33) DATASHEET
Host species: Mouse
Isotype: IgG2a, kappa
Applications: ELISA, WB
Accession: P60709
Overview

Catalog No.

RHF37501

Description

Loading control in ELISA,WB

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Applications

ELISA, WB

Recommended Dilution

Application Dilution
ELISA 1:2000-1:4000, WB: 1:1000-1:4000

Target

Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

Concentration

0.95 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P60709

Form

Liquid

Storage buffer

315mM Arginine, pH 8.0, 173mM H3PO4, 0.043% Tween 20

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0339

Note

For research use only.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with ACTB/β-actin/Beta Actin antibody (RHF37501) at 0.2 μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate
    Lane 4: PC12 cell lysate
    Lane 5: BV2 cell lysate

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 42 kDa
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with ACTB/β-actin/Beta Actin antibody (RHF37501) at 0.2 μg/ml.

    Lane 1: mouse kidney lysate
    Lane 2: mouse lung lysate
    Lane 3: rat kidney lysate
    Lane 4: rat lung lysate

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 42 kDa
  • Western Blot
    Fig. 4. TRAF2 antibody specificity analysis by WB. A: WB results of TRAF2 in ducks incubated by chicken TRAF2 antibody. B: WB results of TRAF2 in mouse incubated by chicken TRAF2 antibody. C: WB results of TRAF2 in rabbit incubated by chicken TRAF2 antibody. D: WB results of TRAF2 in goat incubated by chicken TRAF2 antibody.
    Data sourced from citation (PMID: 39798767)
References

Potential role of TRAF2 in pulmonary hypertension in broiler chickens and preparation and specificity analysis of its polyclonal antibody. [RHF37501]

Characterizing mouse male germ cell-specific actin capping protein alpha3 (CPalpha3): dynamic patterns of expression in testicular and epididymal sperm., PMID:18645673

An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV., PMID:20398263

Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids., PMID:20477829

Identification and validation of candidate biomarkers involved in human ovarian autoimmunity., PMID:21890413

Immunization with ovarian autoantigens leads to reduced fertility in mice following follicular dysfunction., PMID:22143970

Env gene expression of human endogenous retrovirus-k and human endogenous retrovirus-w in childhood acute leukemia cells., PMID:23328642

DDX6 post-transcriptionally down-regulates miR-143/145 expression through host gene NCR143/145 in cancer cells., PMID:23932921

Targeting syndecan-1 in breast cancer inhibits osteoclast functions through up-regulation of osteoprotegerin., PMID:26909290

Clinical relevance of miR-mediated HLA-G regulation and the associated immune cell infiltration in renal cell carcinoma., PMID:26155421

Optimization of Recovery and Analysis of RNA in Sorted Cells in mRNA Quantification After Fluorescence-activated Cell Sorting., PMID:27993868

ATG5 is required for B cell polarization and presentation of particulate antigens., PMID:30196744

Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits., PMID:31234698

Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease., PMID:31462148

Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation., PMID:32019420

The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses., PMID:32249716

Identification of miR-200a-5p targeting the peptide transporter TAP1 and its association with the clinical outcome of melanoma patients., PMID:32923135

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545

TFEB rearranged renal cell carcinoma. A clinicopathologic and molecular study of 13 cases. Tumors harboring MALAT1-TFEB, ACTB-TFEB, and the novel NEAT1-TFEB translocations constantly express PDL1., PMID:33208882

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

Novel serum autoantibodies against ß-actin (ACTB) in amyotrophic lateral sclerosis., PMID:33792444

Circulating Mir-140 and leptin improve the accuracy of the differential diagnosis between psoriatic arthritis and rheumatoid arthritis: a case-control study., PMID:34380068

Routine noninvasive prenatal screening for fetal Rh D in maternal plasma-A 2-year experience from a single center in Belgium., PMID:35352833

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD., PMID:35473441

Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis., PMID:35491858

Low anti-CFL1 antibody with high anti-ACTB antibody is a poor prognostic factor in esophageal squamous cell carcinoma., PMID:35780443

Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells., PMID:35899934

Porcine reproductive and respiratory syndrome virus degrades DDX10 via SQSTM1/p62-dependent selective autophagy to antagonize its antiviral activity., PMID:36779599

Alterations in the expression pattern of RBC membrane associated proteins (RMAPs) in whole body γ-irradiated Sprague Dawley rats., PMID:37315317

Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair., PMID:37978868

Proteomic Analysis of Egg Yolk Proteins During Embryonic Development in Wanxi White Goose., PMID:38433387

Exploring heterogeneous expression of beta-actin (ACTB) in bladder cancer by producing a monoclonal antibody 6D6., PMID:38867273

Si Microanemones Integrated Microfluidic Chip for Highly Efficient Isolation of Extracellular Vesicles., PMID:40395100

Datasheet
$ 130
Product specifications
50 μg 130 100 μg 220

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339) [RHF37501]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only