Catalog No.
KVV13301
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Monkeypox Virus M1R Antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Monkeypox Virus M1R Antigen present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled Monkeypox Virus M1R antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Monkeypox Virus M1R Antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.
Target
M1R
Applications
Used for the quantitative determination of Monkeypox Virus M1R Antigen concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
7.81 - 500 pg/mL
Sensitivity
1.9 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
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|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
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16
|
16
|
16
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24
|
24
|
24
|
|
Mean (pg/mL)
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230.2
|
58.9
|
14.2
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256.3
|
69.5
|
18.8
|
|
Standard deviation
|
7.8
|
1.9
|
1.1
|
24.1
|
7.9
|
2.7
|
|
CV (%)
|
3.4
|
3.2
|
7.7
|
9.4
|
11.4
|
14.4
|
Recovery
85-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Importance of epidemiological research of monkeypox: is incidence increasing? PMID: 32096659
Human Monkey Pox Virus Infection in Plateau State, North Central Nigeria: A Report of Two Cases. PMID: 35038257
Cross-Neutralizing and Protective Human Antibody Specificities to Poxvirus Infections. PMID: 27768891
Monkeypox virus: ecology and public health significance. PMID: 2853010
Inhalational monkeypox virus infection in cynomolgus macaques. PMID: 23061051
Assessing Monkeypox Virus Prevalence in Small Mammals at the Human-Animal Interface in the Democratic Republic of the Congo. PMID: 28972544
Monkeypox: another test for PCR. PMID: 35959687
Guidelines for pregnant individuals with monkeypox virus exposure. PMID: 35750071
Human monkeypox. PMID: 15451482
Possibility of vertical transmission of the human monkeypox virus. PMID: 35963577
A phylogeographic investigation of African monkeypox. PMID: 25912718
Comparison of Multiplexed Immunofluorescence Imaging to Chromogenic Immunohistochemistry of Skin Biomarkers in Response to Monkeypox Virus Infection. PMID: 32717786
Monkeypox virus and whitepox viruses. PMID: 35952
Attenuation of monkeypox virus by deletion of genomic regions. PMID: 25462353
Intranasal monkeypox marmoset model: Prophylactic antibody treatment provides benefit against severe monkeypox virus disease. PMID: 29927927
Factors affecting the likelihood of monkeypox's emergence and spread in the post-smallpox era. PMID: 22709519
Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus-infected cells reveals virus-specific regulation of immune response genes. PMID: 23425254
Recent advances in the diagnosis monkeypox: implications for public health. PMID: 35997157
Co-administration of tecovirimat and ACAM2000鈩?in non-human primates: Effect of tecovirimat treatment on ACAM2000 immunogenicity and efficacy versus lethal monkeypox virus challenge. PMID: 31677948
Sequence of pathogenic events in cynomolgus macaques infected with aerosolized monkeypox virus. PMID: 25653439
Lethal monkeypox virus infection of CAST/EiJ mice is associated with a deficient gamma interferon response. PMID: 22696658
Characterizing monkeypox virus specific CD8+ T cell epitopes in rhesus macaques. PMID: 24210113
Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus. PMID: 27467578
Monkeypox virus as a source of whitepox viruses. PMID: 91589
Monkeypox: a neglected old foe. PMID: 35691304
Monkeypox - Past as Prologue. PMID: 36001716
Monkeypox Rash Severity and Animal Exposures in the Democratic Republic of the Congo. PMID: 31875271
Proteomic basis of the antibody response to monkeypox virus infection examined in cynomolgus macaques and a comparison to human smallpox vaccination. PMID: 21209900
A case of imported Monkeypox in Singapore. PMID: 31657773
Evasion of the Innate Immune Type I Interferon System by Monkeypox Virus. PMID: 26246580
Environmental Persistence of Monkeypox Virus on Surfaces in Household of Person with Travel-Associated Infection, Dallas, Texas, USA, 2021. PMID: 35951009
[Perspectives on recent monkeypox outbreak in non-endemic areas]. PMID: 35872578
Monkeypox virus infection of rhesus macaques induces massive expansion of natural killer cells but suppresses natural killer cell functions. PMID: 24147080
Nanopore sequencing of a monkeypox virus strain isolated from a pustular lesion in the Central African Republic. PMID: 35750759
Epidemiologic and Ecologic Investigations of Monkeypox, Likouala Department, Republic of the Congo, 2017. PMID: 30666937
Experimental infection of prairie dogs with monkeypox virus. PMID: 15829191
Monkeypox Disease Outbreak (2022): Epidemiology, Challenges, and the Way Forward. PMID: 35762024
Monkeypox virus evades antiviral CD4+ and CD8+ T cell responses by suppressing cognate T cell activation. PMID: 18796610
Strengthening of Surveillance during Monkeypox Outbreak, Republic of the Congo, 2017. PMID: 29774865
The monkeypox virus outbreak: reflections from the frontlines. PMID: 14608307
Outbreaks of human monkeypox after cessation of smallpox vaccination. PMID: 22239910
[What are we talking about? When Monkeypox virus infection inadvertently results in smallpox in French]. PMID: 35792844
Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PMID: 26517839
The role of squirrels in sustaining monkeypox virus transmission. PMID: 2820094
Emergence of human monkeypox in west Africa. PMID: 31285141
[Comparative study of variola virus and monkeypox virus interferon-gamma-binding]. PMID: 16358743
[Twelve cases of monkeypox virus outbreak in Bangassou District (Central African Republic) in December 2015]. PMID: 27783372
Monkeypoxvirus infections. PMID: 11189729
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For research use only. Not for human or drug use.
