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Recombinant HPV16 L1/Major capsid protein L1 Protein, N-GST & C-His

Catalog #:   YVV08801 Specific References (109) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: NP_041332.2
Protein length: Ser2-Leu505
Overview

Catalog No.

YVV08801

Expression system

E. coli

Species

Human papillomavirus type 16

Protein length

Ser2-Leu505

Predicted molecular weight

84.36 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

NP_041332.2

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Major capsid protein L1, L1

Data Image
  • SDS-PAGE
    SDS PAGE for Recombinant Human papillomavirus type 16/HPV16 L1/Major capsid protein L1 Protein
References

Mutation Profile of HPV16 L1 and L2 Genes in Different Geographic Areas. PMID: 36680181

Anti-L1 antibody-bound HPV16 pseudovirus is degraded intracellularly via TRIM21/proteasomal pathway. PMID: 35619167

HPV16 L1 diversity and its potential impact on the vaccination-induced immunity. PMID: 32304786

Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy. PMID: 34635698

Correlation between cervical HPV DNA detection and HPV16 seroreactivity measured with L1-only and L1+L2 viral capsid antigens. PMID: 32510304

HPV16-L1-specific antibody response is associated with clinical remission of high-risk HPV-positive early dysplastic lesions. PMID: 25202103

High levels of HPV16-L1 antibody but not HPV16 DNA load or integration predict oropharyngeal patient outcome: The Papillophar study. PMID: 35199231

Correlation of HPV16 L1 capsid protein expression in cervical dysplasia with HPV16 DNA concentration, HPV16 E6*I mRNA and histologic outcome. PMID: 19697723

HPV16 E5 Mediates Resistance to PD-L1 Blockade and Can Be Targeted with Rimantadine in Head and Neck Cancer. PMID: 31848196

Controlled Hybrid-Assembly of HPV16/18 L1 Bi VLPs in Vitro. PMID: 27998118

Heterogeneous Nuclear Ribonucleoprotein C Proteins Interact with the Human Papillomavirus Type 16 (HPV16) Early 3'-Untranslated Region and Alleviate Suppression of HPV16 Late L1 mRNA Splicing. PMID: 25878250

Immunogenicity assessment of HPV16/18 vaccine using the glutathione S-transferase L1 multiplex serology assay. PMID: 25483632

HPV16 L2 improves HPV16 L1 gene delivery as an important approach for vaccine design against cervical cancer. PMID: 26925750

HPV16 L1 and L2 DNA methylation predicts high-grade cervical intraepithelial neoplasia in women with mildly abnormal cervical cytology. PMID: 23335178

Clinicopathological implications of human papilloma virus (HPV) L1 capsid protein immunoreactivity in HPV16-positive cervical cytology. PMID: 24396289

Identification of HPV16's E6 gene in suspected cases of cervical lesions and docking Study of its L1 protein with active components of Echinacea purpurae. PMID: 36032471

E2/E6 ratio and L1 immunoreactivity as biomarkers to determine HPV16-positive high-grade squamous intraepithelial lesions (CIN2 and 3) and cervical squamous cell carcinoma. PMID: 29400024

HPV16 Entry into Epithelial Cells: Running a Gauntlet. PMID: 34960729

The L1 major capsid protein of HPV16 differentially modulates APC trafficking according to the vaccination or natural infection context. PMID: 21061438

Effect of HPV16 L1 virus-like particles on the aggregation of non-functionalized gold nanoparticles. PMID: 28889068

Exploring the roles of HPV16 variants in head and neck squamous cell carcinoma: current challenges and opportunities. PMID: 34749746

Astaxanthin Prevents Human Papillomavirus L1 Protein Binding in Human Sperm Membranes. PMID: 30400141

Survival-related indicators ALOX12B and SPRR1A are associated with DNA damage repair and tumor microenvironment status in HPV 16-negative head and neck squamous cell carcinoma patients. PMID: 35768785

hnRNP L controls HPV16 RNA polyadenylation and splicing in an Akt kinase-dependent manner. PMID: 28934469

ISA101 and nivolumab for HPV-16+ cancer: updated clinical efficacy and immune correlates of response. PMID: 35193933

The papillomavirus major capsid protein L1. PMID: 23800545

Study results and related evidence do not support use of HPV16 L1 DRH1 antibodies as a cancer screening test. PMID: 33249381

Response to: "Study results and related evidence do not support use of HPV16 L1 DRH1 antibodies as a cancer screening test". PMID: 33254024

A colorimetric aptasensor for the simple and rapid detection of human papillomavirus type 16 L1 proteins. PMID: 33688885

Human papillomavirus L1 protein expressed in Escherichia coli self-assembles into virus-like particles that are highly immunogenic. PMID: 27107614

An Examination of HPV16 Natural Immunity in Men Who Have Sex with Men (MSM) in the HPV in Men (HIM) Study. PMID: 29475967

The study of immunogenicity of the antigenic protein of high risk oncogenic type of the human papillomavirus HPV16 L1 produced in the plant expression system on the base of transgenic tomato. PMID: 28726109

Mapping the B cell epitopes within the major capsid protein L1 of human papillomavirus type 16. PMID: 29959021

Humoral Response to HPV16 Proteins in Persons with Anal High-Grade Squamous Intraepithelial Lesion or Anal Cancer. PMID: 32883662

Genetic Variability in the E6, E7, and L1 Genes of Human Papillomavirus Types 16 and 18 among Women in Saudi Arabia. PMID: 36680149

Comparison of multiplex-serology and ELISA based methods in detecting HPV16 L1 antibody responses in paired saliva and serum samples of healthy men. PMID: 31004663

IgG and IgM responses to human papillomavirus L1 virus-like particle as a function of dosing schedule and vaccine formulation. PMID: 31452045

Characterization of major capsid protein (L1) variants of Human papillomavirus type 16 by cervical neoplastic status in Indian women: Phylogenetic and functional analysis. PMID: 31944308

HPV-16 RNA processing. PMID: 18508629

Genetic signatures for lineage/sublineage classification of HPV16, 18, 52 and 58 variants. PMID: 33238224

Effect of alternative aluminum adjuvants on the absorption and immunogenicity of HPV16 L1 VLPs in mice. PMID: 17581283

Human papillomavirus 16 L1 gene methylation as a potential biomarker for predicting anal intraepithelial neoplasia in men who have sex with men (MSM). PMID: 34469465

Methylation of CpG 5962 in L1 of the human papillomavirus 16 genome as a potential predictive marker for viral persistence: A prospective large cohort study using cervical swab samples. PMID: 31856411

Situation of HPV16 E2 gene status during radiotherapy treatment of cervical carcinoma. PMID: 24761916

Enhanced expression of soluble human papillomavirus L1 through coexpression of molecular chaperonin in Escherichia coli. PMID: 26732286

Sex differences in HPV immunity among adults without cancer. PMID: 30650013

Displaying 31RG-1 peptide on the surface of HPV16 L1 by use of a human papillomavirus chimeric virus-like particle induces cross-neutralizing antibody responses in mice. PMID: 29683766

Identification of heterogenous nuclear ribonucleoproteins (hnRNPs) and serine- and arginine-rich (SR) proteins that induce human papillomavirus type 16 late gene expression and alter L1 mRNA splicing. PMID: 34860285

Prevalence and distribution of human papillomavirus (HPV) in Luoyang city of Henan province during 2015-2021 and the genetic variability of HPV16 and 52. PMID: 35246180

The DNA damage response activates HPV16 late gene expression at the level of RNA processing. PMID: 29596642

Critical Residues Involved in the Coassembly of L1 and L2 Capsid Proteins of Human Papillomavirus 16. PMID: 36815785

VLP production in Leishmania tarentolae: A novel expression system for purification and assembly of HPV16 L1. PMID: 26304732

Methylation of HPV16 genome CpG sites is associated with cervix precancer and cancer. PMID: 21306759

Post-treatment human papillomavirus antibody kinetics in cervical cancer patients. PMID: 30955486

Comparative Evaluation of Human Papillomavirus Type 16 L1 Protein Expressed in Plasmid- and Baculovirus-Based Systems in Insect Cells. PMID: 32621447

Development of an IP-Free Biotechnology Platform for Constitutive Production of HPV16 L1 Capsid Protein Using the Pichia pastoris PGK1 Promoter. PMID: 26090426

HPV16 antibodies as risk factors for oropharyngeal cancer and their association with tumor HPV and smoking status. PMID: 25957822

Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection. PMID: 35886898

The HPV transcriptome in HPV16 positive cell lines. PMID: 21439369

All-atom MD simulations of the HPV16 capsid., PMID:40475506

HPV16 neutralizing monoclonal antibodies show evidence for common developmental pathways and public epitopes., PMID:40236113

A novel fluorescence immunoassay for the quantitative detection of HPV16 L1 antibodies in human serum samples using ZnCdSe/ZnS quantum dot-labeled antibodies., PMID:40197040

Genetic analysis of HPV-16 L1 gene mutations and computational screening of therapeutic inhibitors for cervical cancer treatment., PMID:40192871

Molecular epidemiology of human papillomavirus variants in cervical cancer in India., PMID:39913513

Bioinspired synthesis of virus-like particle-templated thin silica-layered nanocages with enhanced biocompatibility and cellular uptake as drug delivery carriers., PMID:39642678

Detection of HPV 16 and 18 L1 genes by a nucleic acid amplification-free electrochemical biosensor powered by CRISPR/Cas9., PMID:39608317

[A fluorescence immunochromatography method for detection of human papillomavirus type 16 E6 and L1 proteins]., PMID:39584350

Glycan-induced structural activation softens the human papillomavirus capsid for entry through reduction of intercapsomere flexibility., PMID:39572555

Design and antiviral assessment of a panel of fusion proteins targeting human papillomavirus type 16., PMID:39453911

Comparative Analysis of HPV16 Variants in the Untranslated Regulatory Region, L1, and E6 Genes among Vaccinated and Unvaccinated Young Women: Assessing Vaccine Efficacy and Viral Diversity., PMID:39339857

Heterogeneity of HPV16 virus-like particles indicates a complex assembly energy surface., PMID:39276669

Engineering of Recombinant Human Papillomavirus 16 L1 Protein for Incorporation with para-Azido-L-Phenylalanine., PMID:39155395

Immunoinformatics approach for design novel multi-epitope prophylactic and therapeutic vaccine based on capsid proteins L1 and L2 and oncoproteins E6 and E7 of human papillomavirus 16 and human papillomavirus 18 against cervical cancer., PMID:39039819

Prevalence of HPV16 L1 protein in oral biopsies: A diagnostic study from Ecuador., PMID:39018933

Genetic variability of human papillomavirus type 18 based on E6, E7 and L1 genes in central China., PMID:38970084

The Micro-Immunotherapy Medicine 2LPAPI® Displays Immune-Modulatory Effects in a Model of Human Papillomavirus Type-16 L1-Protein Capsid-Treated Human Peripheral Blood Mononuclear Cells and Antiproliferative Effects in a Model of Cervical Cancer Cells., PMID:38611099

A validated polyclonal antiserum-based immunoassay for assessment of HPV 16 L1 relative potency., PMID:38340596

Interaction studies between human papillomavirus virus-like particles and laminin 332 by affinity capillary electrophoresis assisted by bio-layer interferometry., PMID:38199121

Nano-Sized Chimeric Human Papillomavirus-16 L1 Virus-like Particles Displaying Mycobacterium tuberculosis Antigen Ag85B Enhance Ag85B-Specific Immune Responses in Female C57BL/c Mice., PMID:37896900

Alum and a TLR7 agonist combined with built-in TLR4 and 5 agonists synergistically enhance immune responses against HPV RG1 epitope., PMID:37798448

Crystal Structures of Plk1 Polo-Box Domain Bound to the Human Papillomavirus Minor Capsid Protein L2-Derived Peptide., PMID:37684534

Design of a Synthetic Long Peptide Vaccine Targeting HPV-16 and -18 Using Immunoinformatic Methods., PMID:37513985

Chimeric Human Papillomavirus-16 Virus-like Particles Presenting HIV-1 P18I10 Peptide: Expression, Purification, Bio-Physical Properties and Immunogenicity in BALB/c Mice., PMID:37175776

A Cas12a ortholog with distinct TTNA PAM enables sensitive detection of HPV16/18., PMID:37159673

Differences in HPV-specific antibody Fc-effector functions following Gardasil® and Cervarix® vaccination., PMID:36922512

Human papilloma virus-16-specific CD8+ T-cell expansions characterize different clinical forms of lichen planus and not lichen sclerosus et atrophicus., PMID:36922453

Critical Residues Involved in the Coassembly of L1 and L2 Capsid Proteins of Human Papillomavirus 16., PMID:36815785

Mutation Profile of HPV16 L1 and L2 Genes in Different Geographic Areas., PMID:36680181

Chimeric Human Papillomavirus-16 Virus-like Particles Presenting P18I10 and T20 Peptides from HIV-1 Envelope Induce HPV16 and HIV-1-Specific Humoral and T Cell-Mediated Immunity in BALB/c Mice., PMID:36679860

Serologic Response to Human Papillomavirus Genotypes Among Unvaccinated Women: Findings From the HITCH Cohort Study., PMID:36322543

Next generation L2-based HPV vaccines cross-protect against cutaneous papillomavirus infection and tumor development., PMID:36263027

Investigation of the Presence of DNA of Highly Pathogenic Human Papillomaviruses in Water Bodies of the Lake Baikal Natural Territory., PMID:35932447

Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection., PMID:35886898

RG2-VLP: a Vaccine Designed to Broadly Protect against Anogenital and Skin Human Papillomaviruses Causing Human Cancer., PMID:35703545

Anti-L1 antibody-bound HPV16 pseudovirus is degraded intracellularly via TRIM21/proteasomal pathway., PMID:35619167

Human Papillomavirus Minor Capsid Protein L2 Mediates Intracellular Trafficking into and Passage beyond the Endoplasmic Reticulum., PMID:35608352

Genetic variability in minor capsid protein (L2 gene) of human papillomavirus type 16 among Indian women., PMID:35552511

Hypochlorous acid as a disinfectant for high-risk HPV: Insight into the mechanism of action., PMID:35277984

Detecting Human Papillomavirus Type 16 in Cervical Cancer Patients with Molecular Variation of Gene L1 in Riau Province Indonesia., PMID:35092375

HPV16 Entry into Epithelial Cells: Running a Gauntlet., PMID:34960729

Identification of heterogenous nuclear ribonucleoproteins (hnRNPs) and serine- and arginine-rich (SR) proteins that induce human papillomavirus type 16 late gene expression and alter L1 mRNA splicing., PMID:34860285

Germline determinants of humoral immune response to HPV-16 protect against oropharyngeal cancer., PMID:34642315

Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy., PMID:34635698

Human Papillomavirus Vaccines., PMID:34590141

Designing a therapeutic and prophylactic candidate vaccine against human papillomavirus through vaccinomics approaches., PMID:34547435

Immunological responses and anti-tumor effects of HPV16/18 L1-L2-E7 multiepitope fusion construct along with curcumin and nanocurcumin in C57BL/6 mouse model., PMID:34516991

Human papillomavirus 16 L1 gene methylation as a potential biomarker for predicting anal intraepithelial neoplasia in men who have sex with men (MSM)., PMID:34469465

In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses., PMID:34308516

Mutation Profiles, Glycosylation Site Distribution and Codon Usage Bias of Human Papillomavirus Type 16., PMID:34209097

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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Recombinant HPV16 L1/Major capsid protein L1 Protein, N-GST & C-His [YVV08801]
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