Catalog No.
YXX06601
Description
Endo S2 recognizes the conserved N-glycans on the Fc region of IgG by hydrolyzing the chitobiose core (at the beta -1,4 linkage between the two N-acetylglucosamines) of the N-glycans.
Biological activity
Unit Definition - One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.
Expression system
E.coli
Species
Streptococcus pyogenes
Protein length
Endo S2/Endo-beta-N-acetylglucosaminidase is cloned from Streptococcus pyogenes and expressed in E.coli.
Predicted molecular weight
114.67 kDa
Nature
Recombinant
Applications
Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.
Target
Endo S2
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from PBS pH7.4,1mM EDTA, 4%trehalose,1% mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Alternative Names
Endo S2, Endo-S2, EndoS2, Endo-beta-N-acetylglucosaminidase
Note
For research use only.
For research use only. Not for human or drug use.
