α-fucosidase/AlfC

Catalog No.

YXX06501

Description

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

Expression system

E.coli

Species

Lacticaseibacillus paracasei (Lactobacillus paracasei)

Protein length

α-fucosidase/AlfC is cloned from Lacticaseibacillus paracasei (Lactobacillus paracasei) and expressed in E.coli.

Predicted molecular weight

63.40 kDa

Nature

Recombinant

Target

α-fucosidase/AlfC

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Applications

α-fucosidase/AlfC is a highly specific exoglycosidase that catalyzes the hydrolysis of alpha-1,6-linked fucose residues from glycoproteins.

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Alternative Names

α-fucosidase, AlfC, Alpha-L-fucosidase, Alpha-fucosidase

Production of recombinant glycosidases fused with Usp45 and SpaX to avoid the purification and immobilization stages., PMID:38581868

Enhanced antibody-defucosylation capability of α-L-fucosidase by proximity-based protein fusion., PMID:36680935

Characterization and stabilization of the α-L-fucosidase set from Lacticaseibacillus rhamnosus INIA P603., PMID:36370158

Comparative studies on the substrate specificity and defucosylation activity of three α-l-fucosidases using synthetic fucosylated glycopeptides and glycoproteins as substrates., PMID:34126284

Structure and dynamics of an α-fucosidase reveal a mechanism for highly efficient IgG transfucosylation., PMID:33277506

Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine., PMID:31937642

Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases., PMID:30196507

Preparative scale purification of fucosyl-N-acetylglucosamine disaccharides and their evaluation as potential prebiotics and antiadhesins., PMID:25977209

Synthesis of fucosyl-N-acetylglucosamine disaccharides by transfucosylation using α-L-fucosidases from Lactobacillus casei., PMID:23542622

Lactobacillus casei ferments the N-Acetylglucosamine moiety of fucosyl-α-1,3-N-acetylglucosamine and excretes L-fucose., PMID:22544237

Document Download

α-fucosidase/AlfC.pdf