TEV Protease

Catalog No.

YVV16201

Biological activity

One unit of TEV Protease cleaves 85% of a 3 μg control substrate in 1 h at 30°C.

Expression system

E.coli

Species

Tobacco etch virus (TEV)

Protein length

TEV Protease is cloned from Tobacco etch virus (TEV) and expressed in E.coli.

Predicted molecular weight

54.31 kDa

Nature

Recombinant

Applications

TEV Protease is a highly specific cysteine protease. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ↓(S/G/A/M/C/H).

Target

TEV Protease

Concentration

17000U/mL

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Form

Liquid

Storage buffer

25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM EDTA, 50% glycerol.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Alternative Names

TEV Protease, Genome polyprotein

Note

For research use only.

Toehold switch plus signal amplification enables rapid detection., PMID:37641181

Phage Display of Two Distinct Warheads to Inhibit Challenging Proteins., PMID:37682047

Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct., PMID:37822550

Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in Escherichia coli and One-Step Chromatographic Purification., PMID:37892190

Genomic stability of self-inactivating rabies., PMID:37921437

Protein-Protein Interaction: Tandem Affinity Purification in Bacteria., PMID:37930536

Chemoproteomics identifies proteoform-selective caspase-2 inhibitors., PMID:37961563

Corrigendum: The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper., PMID:38026885

Expression and Purification of Recombinant Human Mitochondrial RNA Polymerase (POLRMT) and the Initiation Factors TFAM and TFB2M., PMID:38094251

Improving Protein Expression, Stability, and Function with ProteinMPNN., PMID:38194293

Catalytic activity and autoprocessing of murine caspase-11 mediate noncanonical inflammasome assembly in response to cytosolic LPS., PMID:38231198

Development of a tag-free plant-made interferon gamma production system with improved therapeutic efficacy against viruses., PMID:38274005

Mechanism of Mutation-Induced Effects on the Catalytic Function of TEV Protease: A Molecular Dynamics Study., PMID:38474583

P1' specificity of the S219V/R203G mutant tobacco etch virus protease., PMID:38666764

Unexpected tobacco etch virus (TEV) protease cleavage of recombinant human proteins., PMID:38679188

Chemoproteomics Identifies State-Dependent and Proteoform-Selective Caspase-2 Inhibitors., PMID:38787738

A novel method for synthesizing authentic SARS-CoV-2 main protease., PMID:38852715

Protease-Responsive Toolkit for Conditional Targeted Protein Degradation., PMID:38889440

Surface display provides an efficient expression system for production of recombinant proteins and bacterial whole cell biosensor in E. coli., PMID:38964699

Producing recombinant proteins in Vibrio natriegens., PMID:39049057

Developing Device of Death Operation (DODO) to Detect Apoptosis in 2D and 3D Cultures., PMID:39056805

Discovery of Thioether-Cyclized Macrocyclic Covalent Inhibitors by mRNA Display., PMID:39136646

Characterizing interactions of endoplasmic reticulum resident proteins in situ through the YST-PPI method., PMID:39212204

A unified purification method for actin-binding proteins using a TEV-cleavable His-Strep-tag., PMID:39224451

Novel gnd_v2 Fusion Tag and Engineered TEV Protease Enable Efficient Production of Brazzein., PMID:39300970

Light-triggered protease-mediated release of actin-bound cargo from synthetic cells., PMID:39314483

Production of Tobacco Etch Virus Protease (TEV) Expressed in the Endotoxin-Free Bacillus subtilis and Its Application., PMID:39322786

A method for producing protease pS273R of the African swine fever virus., PMID:39326634

Spatiotemporal Control Over Protein Release from Artificial Cells via a Light-Activatable Protease., PMID:39334525

Development of a Chitin-Based Purification System Utilizing Chitin-Binding Domain and Tobacco Etch Virus Protease Cleavage for Efficient Recombinant Protein Recovery., PMID:39340448

Simplifying Recombinant Protein Production: Combining Golden Gate Cloning with a Standardized Protein Purification Scheme., PMID:39363075

Optogenetic Control of the Mitochondrial Protein Import in Mammalian Cells., PMID:39404433

Robust approach for production of the human oncology target Aurora kinase B in complex with its binding partner INCENP., PMID:39424257

Recombinant Plasminogen Activator of the Sandworm (Perinereis aibuhitensis) Expression in Escherichia coli., PMID:39451405

Optimization of the Production of Soluble Recombinant TEV Protease in Two E. coli Strains., PMID:39606681

Improvements in large-scale production of tobacco etch virus protease., PMID:39681152

Light-Triggered Protease-Mediated Release of Actin-Bound Cargo from Synthetic Cells., PMID:39825686

An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents., PMID:39969093

Interfacing bacterial microcompartment shell proteins with genetically encoded condensates., PMID:39969154

Identification and characterization of a novel haemolytic and haemagglutinating bifunctional lectin from the coral Acropora millepora., PMID:39969171

An activation-based high throughput screen identifies caspase-10 inhibitors., PMID:40013156

Light-induced programmable solid-liquid phase transition of biomolecular condensates for improved biosynthesis., PMID:40082181

PERRC: Protease Engineering with Reactant Residence Time Control., PMID:40093119

Immobilization of alginate C-5 epimerases using Bacillus subtilis spore display., PMID:40178254

Towards a Survival-Based Cellular Assay for the Selection of Protease Inhibitors in Escherichia coli., PMID:40227283

CRISPR-Editing of the Vero Cell Line Improves Processability of Live Virus Vaccines by Enabling Targeted Proteolysis of Fibronectin., PMID:40364716

PERRC: Protease Engineering with Reactant Residence Time Control., PMID:40388903

Enzyme-Based Anti-Inflammatory Therapeutics for Inflammatory Diseases., PMID:40430897

An efficient strategy for producing RNA-free Nucleocapsid protein of SARS-CoV-2 for biochemical and structural investigations., PMID:40490992

Tandem Affinity Purification of Virus-Host Protein Complexes via the Strep/FLAG (SF)-TAP Tag., PMID:40515900

Document Download

TEV Protease.pdf