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Anti-PPL Polyclonal Antibody (PHA91001)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:O60437
Overview

Catalog No.

PHA91001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPL (Glu1439-Gly1716).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

190 kDa paraneoplastic pemphigus antigen,Periplakin,195 kDa cornified envelope precursor protein,KIAA0568,PPL

Purification

Purified by antigen affinity column.

Accession

O60437

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PPL antibody (PHA91001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 35 kDa
    Observed MW: 35 kDa
  • Immunocytochemistry/ Immunofluorescence
    PPL in HaCat Cell Line.
    The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PPL (PHA91001) at 7.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet

Document Download

Anti-PPL Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-PPL Polyclonal Antibody [PHA91001]
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