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Anti-PPL Polyclonal Antibody

Catalog #:   PHA91001 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O60437
Overview

Catalog No.

PHA91001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPL (Glu1439-Gly1716).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

190 kDa paraneoplastic pemphigus antigen,Periplakin,195 kDa cornified envelope precursor protein,KIAA0568,PPL

Purification

Purified by antigen affinity column.

Accession

O60437

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PPL antibody (PHA91001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 35 kDa
    Observed MW: 35 kDa
  • Immunocytochemistry/ Immunofluorescence
    PPL in HaCat Cell Line.
    The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PPL (PHA91001) at 7.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Serum periplakin as a potential biomarker for urothelial carcinoma of the urinary bladder., PMID:25520130

Treatment of murine mast cells with IgEκ and protein L enhances apoptotic cell death induced by IL-3 withdrawal., PMID:25522877

Robust vaginal colonization of macaques with a novel vaginally disintegrating tablet containing a live biotherapeutic product to prevent HIV infection in women., PMID:25875100

Origin and levels of circulating microparticles in normal pregnancy: A longitudinal observation in healthy women., PMID:26067611

Ranibizumab Plus Combined Surgery for Treatment of Neovascular Glaucoma with Vitreous Hemorrhage., PMID:26228222

A method to confer Protein L binding ability to any antibody fragment., PMID:26683650

Expression of Human Immunodeficiency Virus Type 1 Neutralizing Antibody Fragments Using Human Vaginal Lactobacillus., PMID:26950606

Extremities of the N-terminus of envoplakin and C-terminus of its linker subdomain are major epitopes of paraneoplastic pemphigus., PMID:27427435

Click-Chemistry Based Allergen Arrays Generated by Polymer Pen Lithography for Mast Cell Activation Studies., PMID:27511293

A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells., PMID:27603942

Perip7lakin is a target for autoimmunity in asthma., PMID:27717390

The fingerprint of antimitochondrial antibodies and the etiology of primary biliary cholangitis., PMID:28100006

Effect of a cocoa diet on the small intestine and gut-associated lymphoid tissue composition in an oral sensitization model in rats., PMID:28189917

Clickable Antifouling Polymer Brushes for Polymer Pen Lithography., PMID:28296390

Utility of basophil activation testing to assess perioperative anaphylactic reactions in real-world practice., PMID:28580612

Eculizumab decreases the procoagulant activity of extracellular vesicles in paroxysmal nocturnal hemoglobinuria: A pilot prospective longitudinal clinical study., PMID:28646725

Persistent microglial activation and synaptic loss with behavioral abnormalities in mouse offspring exposed to CASPR2-antibodies in utero., PMID:28755208

Molecular Modeling of the Interaction of Protein L with Antibodies., PMID:31457247

Postprandial Increase in Blood Plasma Levels of Tissue Factor-Bearing (and Other) Microvesicles Measured by Flow Cytometry: Fact or Artifact?, PMID:31249938

Penicillin minor determinants: History and relevance for current diagnosis., PMID:30248407

Optimization of Zika virus envelope protein production for ELISA and correlation of antibody titers with virus neutralization in Mexican patients from an arbovirus endemic region., PMID:30587198

Prospective and comparative study of paroxysmal nocturnal hemoglobinuria patients treated or not by eculizumab: Focus on platelet extracellular vesicles., PMID:31277120

The Science Underlying COVID-19: Implications for the Cardiovascular System., PMID:32293910

Exploration and Modulation of Antibody Fragment Biophysical Properties by Replacing the Framework Region Sequences., PMID:32326443

Minimal agreement between basophil activation test and immunoassay in diagnosis of penicillin allergy., PMID:32532468

Circulating Rather Than Intestinal PCSK9 (Proprotein Convertase Subtilisin Kexin Type 9) Regulates Postprandial Lipemia in Mice., PMID:32673528

Primary pulmonary diffuse large B-cell lymphoma with multiple ground-glass nodules as the primary manifestation: A case report., PMID:33327289

Week 2 Symptomatic Response with Vedolizumab as a Predictive Factor in Japanese Anti-TNFα-Naive Patients with Ulcerative Colitis: A post hoc Analysis of a Randomized, Placebo-Controlled Phase 3 Trial., PMID:33454706

Distinguishing paraneoplastic pemphigus with keratotic skin lesions lacking anti-plakin antibodies from severe lichen planus., PMID:33871365

More Than Meets the Kappa for Antibody Superantigen Protein L (PpL)., PMID:35225872

B cell receptor signaling strength modulates cancer immunity., PMID:35289309

Variable-heavy (VH) families influencing IgA1&2 engagement to the antigen, FcαRI and superantigen proteins G, A, and L., PMID:35444201

Changes in glycans on platelet microparticles released during storage of apheresis platelets are associated with phosphatidylserine externalization and phagocytosis., PMID:35467785

Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand., PMID:36206851

Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure., PMID:39196168

A Novel Shigella O-Polysaccharide-IpaB Conjugate Vaccine Elicits Robust Antibody Responses and Confers Protection against Multiple Shigella Serotypes., PMID:37017547

Autoantibodies are associated with disease progression in idiopathic pulmonary fibrosis., PMID:37080573

A sensitive tissue factor activity assay determined by an optimized thrombin generation method., PMID:37467256

Recruitment into antibody prevalence studies: a randomized trial of postcards vs. letters as invitations., PMID:37481522

Development of Prodrug-Payloads for Targeted Therapeutic Applications of Platinum-Acridine Anticancer Agents., PMID:37813818

Identifying the in vivo-induced antigenic genes is a strategy to develop DNA vaccine against Nocardia seriolae in hybrid snakehead (Channa maculata ♀ × Channa argus ♂)., PMID:38309489

Primary pancreatic peripheral T-cell lymphoma: A case report., PMID:38660638

PD-L1 targeted peptide demonstrates potent antitumor and immunomodulatory activity in cancer immunotherapy., PMID:38745661

Cabozantinib and nivolumab with or without live bacterial supplementation in metastatic renal cell carcinoma: a randomized phase 1 trial., PMID:38942995

Efficacy and safety of faricimab for neovascular age-related macular degeneration: a systematic review and network meta-analysis., PMID:39043575

Long-term efficacy and safety of danicopan as add-on therapy to ravulizumab or eculizumab in PNH with significant EVH., PMID:39700502

A broad spectrum Shigella vaccine based on VirG53-353 multiepitope region produced in a cell-free system., PMID:39805874

[Primary Pancreatic Lymphoma Coexisting with Intraductal Papillary Mucinous Neoplasm-A Case Report]., PMID:39949016

Effect of alirocumab on postprandial hyperlipidaemia in patients with type 2 diabetes: A randomized, double-blind, placebo-controlled, cross-over trial., PMID:40045751

Increasing the developability of lambda chain antibodies: engineering to confer protein L binding activity and its consequences., PMID:40368210

Datasheet

Document Download

Anti-PPL Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-PPL Polyclonal Antibody [PHA91001]
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