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Anti-GDF9 Polyclonal Antibody

Catalog #:   PHA89801 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O60383
Overview

Catalog No.

PHA89801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GDF9 (Gly320-Arg454).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Growth/differentiation factor 9,GDF-9,GDF9

Purification

Purified by antigen affinity column.

Accession

O60383

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with GDF9 antibody (PHA89801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 18 kDa
    Observed MW: 18 kDa
References

Roles of GDF9 and BMP15 in equine follicular development: in vivo content and in vitro effects of IGF1 and cortisol on granulosa cells., PMID:40289073

Isolation of female germline stem cells from neonatal piglet ovarian tissue and differentiation into oocyte-like cells., PMID:36525858

Enhanced method to select human oogonial stem cells for fertility research., PMID:34415395

New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause., PMID:33772413

Activation-induced cytidine deaminase is a possible regulator of cross-talk between oocytes and granulosa cells through GDF-9 and SCF feedback system., PMID:33589683

Eukaryotic expression, Co-IP and MS identify BMPR-1B protein-protein interaction network., PMID:32471519

Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes., PMID:30388311

Embryonic poly(A)-binding protein is differently expressed and interacts with the messenger RNAs in the mouse oocytes and early embryos., PMID:30302808

Enrichment of Female Germline Stem Cells from Mouse Ovaries Using the Differential Adhesion Method., PMID:29723864

Effect of human ZP3 monoclonal antibody on expression of GDF-9 and number of theca cells in ovary of mice (Mus musculus)., PMID:31435327

In vitro differentiation of human oocyte-like cells from oogonial stem cells: single-cell isolation and molecular characterization., PMID:29304224

Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling., PMID:29221447

Molecular forms of ruminant BMP15 and GDF9 and putative interactions with receptors., PMID:28733348

Differential expression of GDF-9 and BMP- 15 during follicular development in canine ovaries evaluated by flow cytometry., PMID:26876149

Immune-mediated destruction of ovarian follicles associated with the presence of HSP90 antibodies., PMID:25653208

Beneficial effects of melatonin on bovine oocytes maturation: a mechanistic approach., PMID:25070516

Female reproductive aging is master-planned at the level of ovary., PMID:24788203

Human amniotic fluid stem cells have a potential to recover ovarian function in mice with chemotherapy-induced sterility., PMID:24006896

Expression of growth differentiation factor 9 (GDF-9) during in vitro maturation in canine oocytes., PMID:23849650

Overexpression of Uromodulin-like1 accelerates follicle depletion and subsequent ovarian degeneration., PMID:23190605

Effects of species differences on oocyte regulation of granulosa cell function., PMID:22967842

Regulatory role of kit ligand-c-kit interaction and oocyte factors in steroidogenesis by rat granulosa cells., PMID:22366471

Active immunization against the proregions of GDF9 or BMP15 alters ovulation rate and litter size in mice., PMID:22106408

Inhibition of oocyte growth factors in vivo modulates ovarian folliculogenesis in neonatal and immature mice., PMID:20007639

Effects of active immunization against growth differentiation factor 9 and/or bone morphogenetic protein 15 on ovarian function in cattle., PMID:19439562

The proregion of mouse BMP15 regulates the cooperative interactions of BMP15 and GDF9., PMID:18633140

Fibroblast growth factor 7 stimulates in vitro growth of oocytes originating from bovine early antral follicles., PMID:18386286

Characterization of the post-translational modification of recombinant human BMP-15 mature protein., PMID:18227435

The cooperative effect of growth and differentiation factor-9 and bone morphogenetic protein (BMP)-15 on granulosa cell function is modulated primarily through BMP receptor II., PMID:18063682

Inhibition of premature oocyte maturation: a role for bone morphogenetic protein 15 in zebrafish ovarian follicles., PMID:17656459

Induction of oocyte-like cells from mouse embryonic stem cells by co-culture with ovarian granulosa cells., PMID:17490416

The role of growth differentiation factor-9 (GDF-9) and its analog, GDF-9b/BMP-15, in human breast cancer., PMID:17453295

The effects of immunizing sheep with different BMP15 or GDF9 peptide sequences on ovarian follicular activity and ovulation rate., PMID:17093201

Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters., PMID:16997917

A unique preovulatory expression pattern plays a key role in the physiological functions of BMP-15 in the mouse., PMID:16818886

Cell type- and stage-specific changes in HOXA7 protein expression in human ovarian folliculogenesis: possible role of GDF-9., PMID:16466395

[Protective effect of zuogui pill on ovarian autoimmune injury]., PMID:16313118

Oocytes prevent cumulus cell apoptosis by maintaining a morphogenic paracrine gradient of bone morphogenetic proteins., PMID:16263764

Bone morphogenetic protein 15 and growth differentiation factor 9 co-operate to regulate granulosa cell function., PMID:15798022

Role of oocyte-secreted growth differentiation factor 9 in the regulation of mouse cumulus expansion., PMID:15761035

Expression and function of growth differentiation factor-9 in an oviparous species, Gallus domesticus., PMID:15625233

Immunoneutralization of growth differentiation factor 9 reveals it partially accounts for mouse oocyte mitogenic activity., PMID:15128595

Pregnancy-associated plasma protein-a production in rat granulosa cells: stimulation by follicle-stimulating hormone and inhibition by the oocyte-derived bone morphogenetic protein-15., PMID:15087430

Evaluation of members of the TGFbeta superfamily as candidates for the oocyte factors that control mouse cumulus expansion and steroidogenesis., PMID:14635927

Growth differentiation factor-9 and stem cell factor promote primordial follicle formation in the hamster: modulation by follicle-stimulating hormone., PMID:14585807

Growth differentiation factor-9 induces Smad2 activation and inhibin B production in cultured human granulosa-luteal cells., PMID:12574210

Growth differentiation factor-9 stimulates inhibin production and activates Smad2 in cultured rat granulosa cells., PMID:12488343

Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep., PMID:12444053

Bone morphogenetic protein receptor type II is a receptor for growth differentiation factor-9., PMID:12135884

A negative feedback system between oocyte bone morphogenetic protein 15 and granulosa cell kit ligand: its role in regulating granulosa cell mitosis., PMID:12048244

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-GDF9 Polyclonal Antibody [PHA89801]
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