Catalog No.
YVV15501
Description
Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 minute at 30°C in a total reaction volume of 50 µl.
Expression system
E. coli
Species
Escherichia phage lambda (Bacteriophage lambda)
Protein length
Lambda PP is cloned from Escherichia phage lambda and expressed in E.coli.
Predicted molecular weight
27.32 kDa
Nature
Recombinant
Applications
Remove phosphate groups from phosphorylated serine,threonine and tyrosine residues in proteins.
Target
Lambda Protein Phosphatase (Lambda PP)
Concentration
370U/μl
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Liquid
Storage buffer
100 mM NaCl, 50 mM HEPES, pH 7.5, 0.1 mM MnCl2, 0.1 mM EDTA, 2 mM DTT, 0.01% Brij35, and 50% glycerol.
Precision
Reaction Condition
1X Buffer for Lambda PP
Supplement with 1 mM MnCl2
Incubate at 30°C
Protocol
1. Combine protein sample and H2O (if necessary) to a total volume of 38 µl.
2. Add 5 μL of 10X Buffer for Lambda PP and 5 μL of 10 mM MnCl2 to make a total reaction volume of 50 µl.
3. Add 2 µl of Lambda PP.
4. Incubate at 30°C for 30 minutes.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Note
Avoid repeated freeze/thaw cycles. For research use only.
Comparative anatomy of siphophage tails before and after interaction with their receptor., PMID:40279683
Glutathione S-transferase in mediating adaptive responses of oats (Avena sativa) to osmotic and cadmium stress: genome-wide analysis., PMID:40281415
The ACE2 Receptor from Common Vampire Bat (Desmodus rotundus) and Pallid Bat (Antrozous pallidus) Support Attachment and Limited Infection of SARS-CoV-2 Viruses in Cell Culture., PMID:40284950
Reactive Bcl2-positive germinal centres in paediatric tonsils: expanding the spectrum of lymphoma mimickers in children and adolescents., PMID:40289261
Probing the effect of PEG-DNA interactions and buffer viscosity on tethered DNA in shear flow., PMID:40291666
Plasma Exchange vs. Immunoadsorption: Effects on Immunological Markers and Predictive Value in Steroid-Refractory MS Attacks., PMID:40292037
Proliferative glomerulonephritis with monoclonal immunoglobulin deposition coexisting with ANCA-associated glomerulonephritis: a case report., PMID:40308572
The Pericardium Cells Junctions Are a Target for Autoantibodies of Patients Affected by a Variant of Endemic Pemphigus Foliaceus in El Bagre and Surrounding Municipalities in Colombia, South America., PMID:40310349
BCL-6-Positive Cutaneous Mantle Zone Hyperplasia: A Novel Subtype of B-Cell Cutaneous Lymphoid Hyperplasia., PMID:40321030
Uremic toxins removal and iron status: a medium-term comparison between 4 dialysis techniques (EMPIRE study)., PMID:40321039
[Clinical Characteristics and Prognosis of Patients with IgD Multiple Myeloma]., PMID:40326115
Automated On-the-Fly Optimization of Resource Allocation for Efficient Free Energy Simulations., PMID:40328725
Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ., PMID:40328761
The common genetic variant rs1278960 determining expression of Interferon-lambda predicts inflammatory response in critically ill COVID-19 patients., PMID:40328868
Associations between type III interferons, obesity and clinical severity of COVID-19., PMID:40330483
Analysis of Serotonin Transporter Gene 5-HTTLPR Polymorphism and Its Impact on Personality Traits in a Sample Without Neuropsychiatric or Substance Use Disorders., PMID:40332353
Post-Transplant Lymphoproliferative Disorder Manifesting as Lymphoplasmacytic Lymphoma Accompanying With Hemophagocytic Lymphohistiocytosis., PMID:40336922
Monoclonal gammopathy-associated C3 glomerulonephritis secondary to follicular lymphoma: a case report., PMID:40342409
Effects of λ-cyhalothrin on the behavior and physiology of Leschenault's rousette bat (Rousettus leschenaultii)., PMID:40349820
CRISPRi-mediated repression of three cI repressors induces the expression of three related Neisseria gonorrhoeae bacteriophages., PMID:40353677
[Application of next-generation sequencing technology for the investigation of immunoglobulin variable region characteristics and their prognostic significance in patients with chronic lymphocytic leukemia]., PMID:40355356
Late Presentation of Galloway-Mowat Syndrome (GAMOS) Associated With Membranous Nephropathy: A Case Report., PMID:40357071
Increasing the developability of lambda chain antibodies: engineering to confer protein L binding activity and its consequences., PMID:40368210
On the determinants of electron transfer reorganization energy in a cytochrome P450: cytochrome b5 complex. A combined quantum mechanics and molecular dynamics simulation study., PMID:40377195
The biological characteristics and life table parameters of Plodia interpunctella (Lepidoptera: Pyralidae) reared on different maize varieties., PMID:40377229
Decreasing the pH value would improve the swallowing characteristics of whey protein isolate-enriched thickened fluids for dysphagia populations., PMID:40382072
A constant pH molecular dynamics and experimental study on the effect of different pH on the structure of urease from Sporosarcina pasteurii., PMID:40387959
Physicochemical and functional characteristics of Eucommia ulmoides seed meal protein hydrolysates and their isolates., PMID:40400485
Comparing hemodialysis and hemodiafiltration performance with and without hemoadsorption., PMID:40400788
Case Report: IgG4-RD-related ophthalmopathy combined with monoclonal gammopathy of undetermined significance., PMID:40421008
Association Between IL-28B (rs8099917) and IL-28B (rs12979860) with Predisposition to Diseases Related to the HTLV-1: Systematic Review and Meta-Analysis., PMID:40430790
The role of hydrolysis on the fibrillization of walnut protein: Self-assembly behavior, structural characteristics., PMID:40435653
Type III interferon, age and IFNL gene single nucleotide polymorphisms determine the characteristics of H1N1 influenza infection., PMID:40438107
New Definition of Light Chain Monoclonal Gammopathy of Undetermined Significance., PMID:40440024
Comparison of middle molecule removal with expanded hemodialysis versus haemodiafiltration among Chinese hemodialysis patients., PMID:40441692
Expression level and clinical significance of IL-29 in serum of patients with coronary heart disease., PMID:40448200
Screening of metallohelices for enantioselective targeting SARS-CoV-2 RNA G-quadruplex., PMID:40448502
γδ T cells induced by zoledronate under macrophage-depleted conditions reduce disease severity and parasite number in Eimeria tenella-infected chicks., PMID:40450955
Integration of proteomics and artificial intelligence-driven OCT biomarker analysis in central retinal vein occlusion., PMID:40451318
An Unusual Case of Transthyretin Cardiac Amyloidosis Presenting as Heart Failure With Improved Ejection Fraction Without Left Ventricular Hypertrophy., PMID:40452727
Immunochemical measurement of urinary free light chains and Bence Jones proteinuria., PMID:40459077
Systematic identification of chicken type I and III interferon-stimulated genes with antiviral potential against fowl adenovirus serotype 4., PMID:40479764
DNA Hanger: Surface-Minimized Single-Molecule Immunoassay Platform., PMID:40484729
Interrogation of Interfering Factors in ELISA Detecting Angiotensin Receptor Antibodies and Specificity Validation Using the Adsorption Elution Crossmatch (AXE) Technique., PMID:40484908
Virion stripping: A new role for bacterial proteases., PMID:40492194
FOXJ1 contributes to the resistance of Rhopalosiphum padi to pyrethroids by modulating the expression of P450 genes., PMID:40500042
Transepithelial transport and mechanism of pyrethroids across Caco-2 cells mediated by transporters., PMID:40500064
Persistently increased expression of PKMζ and unbiased gene expression profiles identify hippocampal molecular traces of a long-term active place avoidance memory and 'shadow' proteins., PMID:40502027
Multiple Molecule λ-Dynamics: Probing Drug Resistance with Concurrent Protein and Ligand Perturbations., PMID:40506019
Assessment of pH-Induced Conformational Changes in Whey Protein Isolate-Dextran Conjugate Using Spectral Technology., PMID:40509484
For research use only. Not for human or drug use.
