Catalog No.
YVV15501
Description
Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 minute at 30°C in a total reaction volume of 50 µl.
Expression system
E. coli
Species
Escherichia phage lambda (Bacteriophage lambda)
Protein length
Lambda PP is cloned from Escherichia phage lambda and expressed in E.coli.
Predicted molecular weight
27.32 kDa
Nature
Recombinant
Target
Lambda Protein Phosphatase (Lambda PP)
Concentration
370U/μl
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Applications
Remove phosphate groups from phosphorylated serine,threonine and tyrosine residues in proteins.
Form
Liquid
Storage buffer
100 mM NaCl, 50 mM HEPES, pH 7.5, 0.1 mM MnCl2, 0.1 mM EDTA, 2 mM DTT, 0.01% Brij35, and 50% glycerol.
Precision
Reaction Condition
1X Buffer for Lambda PP
Supplement with 1 mM MnCl2
Incubate at 30°C
Protocol
1. Combine protein sample and H2O (if necessary) to a total volume of 38 µl.
2. Add 5 μL of 10X Buffer for Lambda PP and 5 μL of 10 mM MnCl2 to make a total reaction volume of 50 µl.
3. Add 2 µl of Lambda PP.
4. Incubate at 30°C for 30 minutes.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
For research use only. Not for human or drug use.
