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Anti-PDHX Polyclonal Antibody

Catalog #:   PHA09501 Specific References (18) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O00330
Overview

Catalog No.

PHA09501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PDHX (Val220-Thr468).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

E3-binding protein,PDX1,Pyruvate dehydrogenase protein X component,mitochondrial,E3BP,PDHX,proX,Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex,Lipoyl-containing pyruvate dehydrogenase complex component X

Purification

Purified by antigen affinity column.

Accession

O00330

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with PDHX antibody (PHA09501) at 0.99μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: LOVO cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 54 kDa
    Observed MW: 54 kDa
  • Immunocytochemistry/ Immunofluorescence
    PDHX in A2780 Cell Line.
    The A2780 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PDHX (PHA09501) at 19.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PDHX antibody (PHA09501) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 29 kDa
    Observed MW: 29 kDa
References

H2-Based Electrochemical Biosensor with Pd Nanowires@ZIF-67 Molecular Sieve Bilayered Sensing Interface for Immunoassay., PMID:31436433

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance., PMID:30786811

Mining the pre-diagnostic antibody repertoire of TgMMTV-neu mice to identify autoantibodies useful for the early detection of human breast cancer., PMID:24886063

PDC-E3BP is not a dominant T-cell autoantigen in primary biliary cirrhosis., PMID:16629643

Autoreactive responses to pyruvate dehydrogenase complex in the pathogenesis of primary biliary cirrhosis., PMID:10807520

The peculiar autoimmunity of primary biliary cirrhosis., PMID:10807519

Primary biliary cirrhosis: an orchestrated immune response against epithelial cells., PMID:10807518

In situ nucleic acid detection of PDC-E2, BCOADC-E2, OGDC-E2, PDC-E1alpha, BCOADC-E1alpha, OGDC-E1, and the E3 binding protein (protein X) in primary biliary cirrhosis., PMID:10385636

Characterization of the autoantibody responses to recombinant E3 binding protein (protein X) of pyruvate dehydrogenase in primary biliary cirrhosis., PMID:10385634

Autoepitope mapping and reactivity of autoantibodies to the dihydrolipoamide dehydrogenase-binding protein (E3BP) and the glycine cleavage proteins in primary biliary cirrhosis., PMID:10094940

Pyruvate dehydrogenase complex deficiency and absence of subunit X., PMID:9501264

Biochemistry and autoimmune response to the 2-oxoacid dehydrogenase complexes in primary biliary cirrhosis., PMID:9089910

Antibodies to E1 and E2/Protein X components of pyruvate dehydrogenase complex in sera of patients with primary biliary cirrhosis., PMID:9007715

T-cell responses to the components of pyruvate dehydrogenase complex in primary biliary cirrhosis., PMID:7705811

Demonstration of peptide-specific and cross-reactive epitopes in proteins reacting with antimitochondrial antibodies of primary biliary cirrhosis., PMID:1380527

Specific reactivity of recombinant human PDC-E1 alpha in primary biliary cirrhosis., PMID:1797026

Autoantibodies in primary biliary cirrhosis: analysis of reactivity against eukaryotic and prokaryotic 2-oxo acid dehydrogenase complexes., PMID:1999318

Antimitochondrial autoantibodies in primary biliary cirrhosis recognize cross-reactive epitope(s) on protein X and dihydrolipoamide acetyltransferase of pyruvate dehydrogenase complex., PMID:2473022

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-PDHX Polyclonal Antibody [PHA09501]
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