Catalog No.
RGK60101
Species reactivity
Broad-Spectrum
Host species
mouse
Isotype
IgG2a, kappa
Clonality
Monoclonal
Target
Pan-pY, pTyr, Phosphotyrosine
Concentration
1.19 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Applications
IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
4G10
The 4G10, pY20 and p-TYR-100 antibody specificity: profiling by peptide microarrays. PMID: 22178400
Evaluation of different phospho-tyrosine antibodies for label-free phosphoproteomics. PMID: 25890253
Engineering Improved Antiphosphotyrosine Antibodies Based on an Immunoconvergent Binding Motif. PMID: 30398859
Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis. PMID: 29063176
Optimization of immunoaffinity enrichment and detection: toward a comprehensive characterization of the phosphotyrosine proteome of K562 cells by liquid chromatography-mass spectrometry. PMID: 21403953
Immunoaffinity enrichments followed by mass spectrometric detection for studying global protein tyrosine phosphorylation. PMID: 18543961
SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis. PMID: 28092045
Enrichment of phosphotyrosine proteome of human platelets by immunoprecipitation. PMID: 17172697
Regulation of phosphoinositide kinases in T cells. Evidence that phosphatidylinositol 3-kinase is not a substrate for T cell antigen receptor-regulated tyrosine kinases. PMID: 1385426
Identification of the phosphotyrosine proteome from thrombin activated platelets. PMID: 12112843
A sensitive magnetic bead method for the detection and identification of tyrosine phosphorylation in proteins by MALDI-TOF/TOF MS. PMID: 19526547
Involvement of phosphoinositide 3-kinase and its association with pp60src in cholecystokinin-stimulated pancreatic acinar cells. PMID: 11139143
The equatorial subsegment in mammalian spermatozoa is enriched in tyrosine phosphorylated proteins. PMID: 18448843
Melanoma cell spreading on fibronectin induced by 12(S)-HETE involves both protein kinase C- and protein tyrosine kinase-dependent focal adhesion formation and tyrosine phosphorylation of focal adhesion kinase (pp125FAK). PMID: 7593207
Amyloid beta peptide specifically promotes phosphorylation and nuclear translocation of the extracellular signal-regulated kinase in cultured rat cortical astrocytes. PMID: 14646244
Detection of tyrosine phosphorylated proteins by combination of immunoaffinity enrichment, two-dimensional difference gel electrophoresis and fluorescent Western blotting. PMID: 20888324
Alteration of the PKC theta-Vav1 complex and phosphorylation of Vav1 in TCDD-induced apoptosis in the lymphoblastic T cell line, L-MAT. PMID: 20561557
A cyclic adenosine 3',5'-monophosphate-induced tyrosine phosphorylation of Syk protein tyrosine kinase in the flagella of boar spermatozoa. PMID: 15457545
Photodynamic therapy inhibits cell adhesion without altering integrin expression. PMID: 9434126
Mechanism of changes induced in plasma glycerol by scent stimulation with grapefruit and lavender essential oils. PMID: 17376592
Reactive oxygen species promote tyrosine phosphorylation and capacitation in equine spermatozoa. PMID: 14511778
A chlamydial type III translocated protein is tyrosine-phosphorylated at the site of entry and associated with recruitment of actin. PMID: 15199184
Interleukin-5 signaling in human eosinophils involves JAK2 tyrosine kinase and Stat1 alpha. PMID: 7888666
Altered tyrosine phosphorylation of ERK1 MAP kinase and other macrophage molecules caused by Leishmania amastigotes. PMID: 10477171
Monoclonal antibody binding to a receptor on nonspecific cytotoxic cells (NCC) increases the expression of proto-oncogene kinases and protein kinase C. PMID: 8562307
Transforming growth factor(beta)-mediated corneal myofibroblast differentiation requires actin and fibronectin assembly. PMID: 10440249
The DNA binding activity of the RIPE3b1 transcription factor of insulin appears to be influenced by tyrosine phosphorylation. PMID: 11309378
Endotoxin tolerance alters phospholipase C-gamma 1 and phosphatidylinositol-3'-kinase expression in peritoneal macrophages. PMID: 7791333
Intracellular signaling factors--enhanced hepatic nuclear protein binding to TTGGC sequence in the rat regucalcin gene promoter: involvement of protein phosphorylation. PMID: 11112452
Regulation of eicosanoid biosynthesis in the macrophage. Involvement of protein tyrosine phosphorylation and modulation by selective protein tyrosine kinase inhibitors. PMID: 8442770
Tyrosine phosphorylation of the NR2B subunit of the NMDA receptor in the spinal cord during the development and maintenance of inflammatory hyperalgesia. PMID: 12122079
Increased basal and induced tyrosine phosphorylation of the insulin-like growth factor I receptor beta subunit in circulating mononuclear cells of patients with polycythemia vera. PMID: 7542500
Differential activation of the Stat signaling pathway in the liver after burn injury. PMID: 9374714
Different proteins are phosphorylated under acidic environments in Jurkat cells. PMID: 12008047
Abnormal tyrosine phosphorylation linked to a defective interaction between ADP and its receptor on platelets. PMID: 9759628
Phosphorylation of the human estrogen receptor on tyrosine 537 in vivo and by src family tyrosine kinases in vitro. PMID: 7539106
Epidermal growth factor receptor participates in growth hormone signaling pathway in cardiac myocytes of neonatal rat. PMID: 11601336
The activation of MAP kinase during vertical palatal shelf development in hamster. PMID: 9129968
For research use only. Not for human or drug use.
