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Anti-Human CD99 Antibody (12E7#)

Catalog #:   FHD03610 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1, kappa
Applications: FCM
Accession: P14209
Overview

Catalog No.

FHD03610

Species reactivity

Human

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

FCM

Target

MIC2Y, E2 antigen, MIC2X, 12E7, Protein MIC2, CD99 antigen, MIC2, T-cell surface glycoprotein E2, CD99

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P14209

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

12E7#

Note

For research use only.

Data Image
  • Bioactivity
    Detects CD99 in indirect ELISAs.
  • Flow-cytometry
    Flow-cytometry using anti-human CD99 antibody.CD99 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # FHD03610 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human CD99 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # FHD03610 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human CD99 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # FHD03610 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts., PMID:31510956

Autoantibodies as diagnostic markers and potential drivers of inflammation in ulcerative colitis., PMID:32050001

A potential vaccine candidate towards chicken coccidiosis mediated by recombinant Lactobacillus plantarum with surface displayed EtMIC2 protein., PMID:32525007

Anti-CD99 scFv-ELP nanoworms for the treatment of acute myeloid leukemia., PMID:32535112

Immunological evaluation of invasive Lactobacillus plantarum co-expressing EtMIC2 and chicken interleukin-18 against Eimeria tenella., PMID:32715344

NKX3.1 immunoreactivity is not identified in mesenchymal chondrosarcoma: a 25-case cohort study., PMID:32779239

Primary Ewing's sarcoma of the lung: a rare case report., PMID:33061150

Anti-human CD99 antibody exerts potent antitumor effects in mantle cell lymphoma., PMID:33215253

Analysis of 24 cases of epithelioid glioblastoma: Experience from a tertiary centre of North India., PMID:33341703

Primary diffuse large B cell lymphoma of the lactating left breast. A case report and review of the literature., PMID:33427203

Clofarabine induces ERK/MSK/CREB activation through inhibiting CD99 on Ewing sarcoma cells., PMID:34133426

Comparative microsomal proteomics of a model lung cancer cell line NCI-H23 reveals distinct differences between molecular profiles of 3D and 2D cultured cells., PMID:34611477

[Extraskeletal mesenchymal chondrosarcoma in central nerve system: a clinicopathological analysis]., PMID:34619870

CAR T cells targeting CD99 as an approach to eradicate T-cell acute lymphoblastic leukemia without normal blood cells toxicity., PMID:34627328

Flow Cytometry Immunophenotyping for Diagnostic Orientation and Classification of Pediatric Cancer Based on the EuroFlow Solid Tumor Orientation Tube (STOT)., PMID:34638431

Targeting CD99 Compromises the Oncogenic Effects of the Chimera EWS-FLI1 by Inducing Reexpression of Zyxin and Inhibition of GLI1 Activity., PMID:34667115

A minute primary gastric synovial sarcoma with ulcer: a case report., PMID:34895269

CD99 antibody disrupts T-cell acute lymphoblastic leukemia adhesion to meningeal cells and attenuates chemoresistance., PMID:34934147

High-valency Anti-CD99 Antibodies Toward the Treatment of T Cell Acute Lymphoblastic Leukemia., PMID:34958778

Optimization of culture conditions for stable expression of recombinant fc-fused human extracellular CD99 in HEK293T cells., PMID:35988884

Serum peptidome profiles immune response of COVID-19 Vaccine administration., PMID:36091008

Genetic background of anti-Xga producers in Japanese blood donors., PMID:36102098

Cell Adhesion Molecule CD99 in Cancer Immunotherapy., PMID:36214301

Chondrosarcoma with Target-Like Chondrocytes: Update on Molecular Profiling and Specific Morphological Features., PMID:36689318

Development and application of an indirect ELISA for the serological detection of bovine viral diarrhea virus infection based on the protein E2 antigen., PMID:36849860

Canine Mammary Neoplasia Induces Variations in the Peripheral Blood Levels of CD20, CD45RA, and CD99., PMID:37298173

A novel TPD52L2-ROS1 gene fusion expanding the molecular alterations in inflammatory myofibroblastic tumor: case report and literature review., PMID:37735390

FCM marker importance for MRD assessment in T-cell acute lymphoblastic leukemia: An AIEOP-BFM-ALL-FLOW study group report., PMID:37776305

Surface and Global Proteome Analyses Identify ENPP1 and Other Surface Proteins as Actionable Immunotherapeutic Targets in Ewing Sarcoma., PMID:37812652

Expression of NKX2.2 in Non-Ewing Tumors With Round Cell Morphology., PMID:38234938

CD99 tumor associated antigen is a potential target for antibody therapy of T-cell acute lymphoblastic leukemia., PMID:38468825

Anti-CD99 Antibody Therapy Triggers Macrophage-Dependent Ewing Cell Death In Vitro and Myeloid Cell Recruitment In Vivo., PMID:38534214

Transcriptome Analysis Reveals the Induction of Apoptosis-Related Genes by a Monoclonal Antibody against a New Epitope of CD99 on T-Acute Lymphoblastic Leukemia., PMID:38804310

An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers., PMID:38904760

FLT3/CD99 Bispecific Antibody-Based Nanoparticles for Acute Myeloid Leukemia., PMID:39007347

Solitary fibrous tumor of thyroid: Case report., PMID:39138014

Rare mediastinal small round cell melanoma with synovial sarcoma-like immunophenotype: A potential diagnostic pitfall., PMID:39151249

Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment., PMID:39167846

Immunophenotypic properties association of CLL and ALL patient cells by flow cytometry analysis., PMID:39397873

A comprehensive analysis of the major specificities of anti-human T lymphocyte porcine immunoglobulin (p-ATG)., PMID:39402313

Exploring the Biological Activity of a Humanized Anti-CD99 ScFv and Antibody for Targeting T Cell Malignancies., PMID:39595598

Plasticity of BioPhi-driven humanness optimization in ScFv-CD99 binding affinity validated through AlphaFold, HADDOCK, and MD simulations., PMID:39897056

Evaluating the immune effector functions induced by humanized anti-CD99 antibody in eliminating T lymphoblastic leukemia/lymphoma cells., PMID:40214906

Inhibition of polymorphonuclear cells averts cytotoxicity against hypoimmune cells in xenotransplantation., PMID:40251154

Combination of anlotinib with immunotherapy enhanced both anti-angiogenesis and immune response in high-grade serous ovarian cancer., PMID:40260248

The Extensive Case Series of 21 CIC::DUX4, 18 BCOR::CCNB3 and 148 Ewing's Sarcomas From a Single Center., PMID:40298239

PILRα on tumor cells interacts with the T cell surface protein CD99 to suppress antitumor immunity., PMID:40312493

Development of an Anti-CD99 Antibody Enables Targeting of Diffuse Midline Glioma., PMID:40424183

CD99: A Key Regulator in Immune Response and Tumor Microenvironment., PMID:40427525

PMN recruitment in inflammatory lung injury models follows classical transendothelial migration paradigms requiring PECAM-1 and CD99., PMID:40445608

Datasheet
$ 198
Product specifications
50 μg 198 100 μg 328

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human CD99 Antibody (12E7#) [FHD03610]
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