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Anti-Human CD162/SELPLG Antibody (SAA0065)

Catalog #:   FHG76510 Specific References (50) DATASHEET
Host species: Human
Isotype: IgG1, kappa
Applications: ELISA, FCM
Accession: Q14242
Overview

Catalog No.

FHG76510

Species reactivity

Human

Host species

Human

Isotype

IgG1, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

Selectin P ligand, PSGL-1, SELPLG, CD162, P-selectin glycoprotein ligand 1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14242

Applications

ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0065

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human CD162 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # FHG76510 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human CD162 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # FHG76510 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human CD162 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # FHG76510 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

PSGL-1 is a phagocytosis checkpoint that enables tumor escape from macrophage clearance., PMID:40512837

A proteogenomic tool uncovers protein markers for human microglial states., PMID:40236015

Association between extracellular vesicles (EVs) and thrombosis in antiphospholipid syndrome., PMID:40123163

Triple knockdown of CD11a, CD49d, and PSGL1 in T cells reduces CAR-T cell toxicity but preserves activity against solid tumors in mice., PMID:39841806

CD93 blockade promotes effector T-cell infiltration and facilitates adoptive cell therapy in solid tumors., PMID:39805660

Comparing methods to detect cellular proteins on the surface of HIV-1 virions., PMID:39647666

Antibody blockade of the PSGL-1 immune checkpoint enhances T-cell responses to B-cell lymphoma., PMID:39455852

Turbinate-homing IgA-secreting cells originate in the nasal lymphoid tissues., PMID:39085603

Endothelial PTP1B Deletion Promotes VWF Exocytosis and Venous Thromboinflammation., PMID:38563147

PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages., PMID:37819238

Extended interval dosing of ocrelizumab modifies the repopulation of B cells without altering the clinical efficacy in multiple sclerosis., PMID:37752582

Low P-Selectin Glycoprotein Ligand-1 Expression in Neutrophils Associates with Disease Activity and Deregulated NET Formation in Systemic Lupus Erythematosus., PMID:37047117

An engineered HIV-1 Gag-based VLP displaying high antigen density induces strong antibody-dependent functional immune responses., PMID:37024469

B-cell receptor associated protein 31 deficiency decreases the expression of adhesion molecule CD11b/CD18 and PSGL-1 in neutrophils to ameliorate acute lung injury., PMID:36210579

Increase of Circulating Monocyte-Platelet Conjugates in Rheumatoid Arthritis Responders to IL-6 Blockage., PMID:35628558

High expression of neutrophil and monocyte CD64 with simultaneous lack of upregulation of adhesion receptors CD11b, CD162, CD15, CD65 on neutrophils in severe COVID-19., PMID:34377464

Phenotypic and Functional Consequences of PLT Binding to Monocytes and Its Association with Clinical Features in SLE., PMID:33947017

Neutrophil-Associated Inflammatory Changes in the Pre-Diabetic Pancreas of Early-Age NOD Mice., PMID:33776903

Relevance of PSGL-1 Expression in B Cell Development and Activation., PMID:33281818

An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA., PMID:32938950

Tissue-resident PSGL1loCD4+ T cells promote B cell differentiation and chronic graft-versus-host disease-associated autoimmunity., PMID:32931481

P-selectin blockade in COVID-19-related ARDS., PMID:32464083

Acidity changes immunology: a new VISTA pathway., PMID:31822869

VISTA is an acidic pH-selective ligand for PSGL-1., PMID:31645726

The role of PSGL-1 in pathogenesis of systemic inflammatory response and coagulopathy in endotoxemic mice., PMID:31450009

Integrins and Their Role in Immune Cell Adhesion., PMID:30952447

Microvascular Mechanisms of Polyphosphate-Induced Neutrophil-Endothelial Cell Interactions in vivo., PMID:30909276

LPS-induced Lung Platelet Recruitment Occurs Independently from Neutrophils, PSGL-1, and P-Selectin., PMID:30768917

Mechanical properties of P-selectin PSGL-1 bonds., PMID:30342396

P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome., PMID:29962384

Inverse Association Between Circulating Monocyte-Platelet Complexes and Inflammation in Ulcerative Colitis Patients., PMID:29529212

N-cadherin knockdown leads to disruption of trophoblastic and endothelial cell interaction in a 3D cell culture model - New insights in trophoblast invasion failure., PMID:29231798

Specific Unbinding Forces Between Mutated Human P-Selectin Glycoprotein Ligand-1 and Viral Protein-1 Measured Using Force Spectroscopy., PMID:29016136

ADAM8 expression in breast cancer derived brain metastases: Functional implications on MMP-9 expression and transendothelial migration in breast cancer cells., PMID:28986926

Activated signature of antiphospholipid syndrome neutrophils reveals potential therapeutic target., PMID:28931754

Targeting P-selectin glycoprotein ligand-1/P-selectin interactions as a novel therapy for metabolic syndrome., PMID:28034759

Soluble P-selectin rescues viper venom-induced mortality through anti-inflammatory properties and PSGL-1 pathway-mediated correction of hemostasis., PMID:27779216

Technical Advance: Changes in neutrophil migration patterns upon contact with platelets in a microfluidic assay., PMID:27630219

Neutrophil Receptor Response to Bacterial N-formyl Peptides is Similar in Term Newborn Infants and Adults in Contrast to IL-8., PMID:27606963

Psgl-1 Deficiency is Protective against Stroke in a Murine Model of Lupus., PMID:27357136

Expression of Inflammation-related Intercellular Adhesion Molecules in Cardiomyocytes In Vitro and Modulation by Pro-inflammatory Agents., PMID:27107077

Selective induction of anti-inflammatory monocyte-platelet aggregates in a model of pulsatile blood flow at low shear rates., PMID:27078265

A Preliminary Comparative Assessment of the Role of CD8+ T Cells in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and Multiple Sclerosis., PMID:26881265

Inhibition of P-Selectin and PSGL-1 Using Humanized Monoclonal Antibodies Increases the Sensitivity of Multiple Myeloma Cells to Bortezomib., PMID:26539491

The Suramin Derivative NF449 Interacts with the 5-fold Vertex of the Enterovirus A71 Capsid to Prevent Virus Attachment to PSGL-1 and Heparan Sulfate., PMID:26430888

Antibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death., PMID:26399965

Deficiency of P-selectin glycoprotein ligand-1 is protective against the prothrombotic effects of interleukin-1β., PMID:26386314

Thromboxane A2 induces blood flow recovery via platelet adhesion to ischaemic regions., PMID:25935870

Soluble P-selectin glycoprotein ligand - a possible new target in ulcerative colitis., PMID:25869560

[Monocytes of human blood as a target of Streptococcus pyogenes components]., PMID:25842951

Datasheet
$ 225
Product specifications
50 μg 225 100 μg 372

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For research use only. Not for human or drug use.

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Anti-Human CD162/SELPLG Antibody (SAA0065) [FHG76510]
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