Catalog No.
CLR00007
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Involvement of p-CREB and phase II detoxifying enzyme system in neuroprotection mediated by the flavonoid calycopterin isolated from Dracocephalum kotschyi., PMID:23639191
The death receptor antagonist FLIP-L interacts with Trk and is necessary for neurite outgrowth induced by neurotrophins., PMID:20427667
The ubiquitin-proteasome system regulates the stability of neuronal nicotinic acetylcholine receptors., PMID:19693707
Characterization of methyl-beta-cyclodextrin toxicity in NGF-differentiated PC12 cell death., PMID:17292476
Propargylamines induce antiapoptotic new protein synthesis in serum- and nerve growth factor (NGF)-withdrawn, NGF-differentiated PC-12 cells., PMID:11961082
Role of caspase 3-dependent Bcl-2 cleavage in potentiation of apoptosis by Bcl-2., PMID:11752215
Nerve growth factor inhibits PC12 cell PDE 2 phosphodiesterase activity and increases PDE 2 binding to phosphoproteins., PMID:11181844
A novel phosphatidylinositol-5-phosphate 4-kinase (phosphatidylinositol-phosphate kinase IIgamma) is phosphorylated in the endoplasmic reticulum in response to mitogenic signals., PMID:9685379
Transport of protein kinase C isoforms to the nucleus of PC12 cells by nerve growth factor: association of atypical zeta-PKC with the nuclear matrix., PMID:9285516
Role of protein kinase C alpha in nerve growth factor-induced arachidonic acid release from PC12 cells., PMID:8780012
Nerve growth factor activates calcium-insensitive protein kinase C-epsilon in PC-12 rat pheochromocytoma cells., PMID:8240290
Characterization and differential expression of protein kinase C isoforms in PC12 cells. Differentiation parallels an increase in PKC beta II., PMID:1544425
Analysis of Fos protein complexes and Fos-related antigens by high-resolution two-dimensional gel electrophoresis., PMID:3325882
For research use only. Not for human or drug use.
