MDA-MB-231 whole cell lysate

Catalog No.

CLH00059

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

The Role of Insulin-like Growth Factor Binding Protein (IGFBP)-2 in DNA Repair and Chemoresistance in Breast Cancer Cells., PMID:38893232

The ancestral type of the R-RAS protein has oncogenic potential., PMID:38383288

2-Difluoromethoxy-Substituted Estratriene Sulfamates: Synthesis, Antiproliferative SAR, Antitubulin Activity, and Steroid Sulfatase Inhibition., PMID:36109340

Sensitive monitoring of doxorubicin in plasma of patients, MDA-MB-231 and 4T1 cell lysates using electroanalysis method., PMID:33120307

Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling., PMID:32575430

Probody Therapeutic Design of 89Zr-CX-072 Promotes Accumulation in PD-L1-Expressing Tumors Compared to Normal Murine Lymphoid Tissue., PMID:31953313

Novel predication of protein biomarkers in interferon-gamma-stimulated breast cancer cells., PMID:30983944

Immunophenotyping of the cluster of differentiation 74, migration inhibitory factor, and cluster of differentiation 44 expression on human breast cancer-derived cell lines., PMID:30983941

Elucidation of Therapeutic Peptide Binding Partners from Isolated Mitochondria., PMID:30397556

Polymeric Nano-Encapsulation of Curcumin Enhances its Anti-Cancer Activity in Breast (MDA-MB231) and Lung (A549) Cancer Cells Through Reduction in Expression of HIF-1α and Nuclear p65 (Rel A)., PMID:29065834

β-Catenin Mediates Anti-adipogenic and Anticancer Effects of Arctigenin in Preadipocytes and Breast Cancer Cells., PMID:28279068

Immunotherapy with dendritic cells and cytokine-induced killer cells for MDA-MB-231 breast cancer stem cells in nude mice., PMID:27508015

Quantitative electrochemical detection of cathepsin B activity in breast cancer cell lysates using carbon nanofiber nanoelectrode arrays toward identification of cancer formation., PMID:25959927

Macroporous reversed-phase separation of proteins combined with reversed-phase separation of phosphopeptides and tandem mass spectrometry for profiling the phosphoproteome of MDA-MB-231 cells., PMID:24888630

Taxol-induced growth arrest and apoptosis is associated with the upregulation of the Cdk inhibitor, p21WAF1/CIP1, in human breast cancer cells., PMID:23023313

Pterostilbene induces mitochondrially derived apoptosis in breast cancer cells in vitro., PMID:22572619

Biomarkers of breast cancer apoptosis induced by chemotherapy and TRAIL., PMID:22133146

Evaluation of tissue factor expression in canine tumor cells., PMID:21801068

Breast carcinoma cell lysate-pulsed dendritic cells cross-prime MUC1-specific CD8+ T cells identified by peptide-MHC-class-I tetramers., PMID:15919376

Identification of tumor-specific antibodies in patients with breast cancer vaccinated with gene-modified allogeneic tumor cells., PMID:12616108

Genistein-induced G2/M arrest is associated with the inhibition of cyclin B1 and the induction of p21 in human breast carcinoma cells., PMID:9664138

Correlation between DNA topoisomerase II activity and cytotoxicity in pMC540 and merodantoin sensitive and resistant human breast cancer cells., PMID:7762997

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