Catalog No.
YXX51101
Description
SUMO protease / ULP1 is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence.
Expression system
E. coli
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein length
SUMO protease is cloned from Baker's yeast and expressed in E.coli.
Predicted molecular weight
27.93 kDa
Nature
Recombinant
Applications
Remove or cleave SUMO tag from the SUMO fusion protein
Target
SUMO protease/ULP1
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Reconstitution
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Note
For research use only.
A new protease required for cell-cycle progression in yeast., PMID:10094048
A new 30-kDa ubiquitin-related SUMO-1 hydrolase from bovine brain., PMID:10531301
Differential regulation of sentrinized proteins by a novel sentrin-specific protease., PMID:10652325
The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein., PMID:10713161
A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex., PMID:15296745
The SUMO-specific protease SENP5 is required for cell division., PMID:16738315
Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes., PMID:17538013
Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system., PMID:18831987
Molecular cloning, expression, and bioactivity of dove B lymphocyte stimulator (doBAFF)., PMID:19117613
High-level expression and purification of heparin-binding epidermal growth factor (HB-EGF) with SUMO fusion., PMID:19950003
A novel mechanism for SUMO system control: regulated Ulp1 nucleolar sequestration., PMID:20647537
Expression and purification of an antitumor-analgesic peptide from the venom of Mesobuthus martensii Karsch by small ubiquitin-related modifier fusion in Escherichia coli., PMID:20945481
Sumo-dependent substrate targeting of the SUMO protease Ulp1., PMID:22034919
Production and characterization of hirudin variant-1 by SUMO fusion technology in E. coli., PMID:22371262
The nuclear pore regulates GAL1 gene transcription by controlling the localization of the SUMO protease Ulp1., PMID:24074957
Regulation of Toll signaling and inflammation by β-arrestin and the SUMO protease Ulp1., PMID:24077307
A new fusion protein platform for quantitatively measuring activity of multiple proteases., PMID:24649897
A roadmap to understanding toll pathway changes: an educational primer for use with "regulation of toll signaling and inflammation by β-arrestin and the SUMO protease Ulp1"., PMID:24723732
Nuclear pore components affect distinct stages of intron-containing gene expression., PMID:25845599
Intron or no intron: a matter for nuclear pore complexes., PMID:26709543
Heterologous expression and purification of neurotoxic Hainantoxin-III in E. coli., PMID:27249514
Immobilization of Ulp1 protease on NHS-activated Sepharose: a useful tool for cleavage of the SUMO tag of recombinant proteins., PMID:28432497
Aggregation kinetics in the presence of brain lipids of Aβ(1-40) cleaved from a soluble fusion protein., PMID:29526708
A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits., PMID:29695777
Rapid and efficient purification of Drosophila homeodomain transcription factors for biophysical characterization., PMID:30738927
The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal., PMID:33159083
Profiling Substrate Specificity of the SUMO Protease Ulp1 by the YESS-PSSC System to Advance the Conserved Mechanism for Substrate Cleavage., PMID:36293045
Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs., PMID:36417751
Reining in Cas13a activity with N-terminal removable tags expands Cas13a based molecular sensing and enables precise gene interference., PMID:36809733
SUMOylation regulates Lem2 function in centromere clustering and silencing., PMID:37970674
SUMO protease and proteasome recruitment at the nuclear periphery differently affect replication dynamics at arrested forks., PMID:38917328
Bicistronic Vector Expression of Recombinant Jararhagin-C and Its Effects on Endothelial Cells., PMID:39728782
For research use only. Not for human or drug use.
