Catalog No.
YXX05001
Description
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments.
Biological activity
One unit will cleave ≥95% of 1μg of recombinant monoclonal IgG in 30 minutes at 37°C.
Expression system
E. coli
Species
Streptococcus pyogenes
Protein length
IdeS Protease is cloned from Streptococcus pyogenes and expressed in E.coli.
Predicted molecular weight
37.57 kDa
Nature
Recombinant
Target
IdeS Protease (IgG Specific)
Concentration
40000U/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Applications
Proteomics
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% glycerol
Experimental Procedure
Digestion reaction condition
37℃, 30min
Protocol
1. Add appropriate amount of IgG (to 5mg) in digestive juice;
2. Add IdeS protease to IgG samples: add 1 unit of IdeS per 1ug of IgG;
3. Incubate the sample at 37°C for 30-60min.
* IdeS proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50 mM sodium phosphate and 150 mM NaCl (pH 6.6), but most common biological buffers are suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
For research use only. Not for human or drug use.
