IdeS Protease (IgG specific)

Catalog No.

YXX05001

Description

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments.

Biological activity

One unit will cleave ≥95% of 1μg of recombinant monoclonal IgG in 30 minutes at 37°C.

Expression system

E. coli

Species

Streptococcus pyogenes

Protein length

IdeS Protease is cloned from Streptococcus pyogenes and expressed in E.coli.

Predicted molecular weight

37.57 kDa

Nature

Recombinant

Target

IdeS Protease (IgG Specific)

Concentration

40000U/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Applications

Proteomics

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% glycerol

Experimental Procedure

Digestion reaction condition

37℃, 30min

 

Protocol

1. Add appropriate amount of IgG (to 5mg) in digestive juice;

2. Add IdeS protease to IgG samples: add 1 unit of IdeS per 1ug of IgG;

3. Incubate the sample at 37°C for 30-60min.

* IdeS proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50 mM sodium phosphate and 150 mM NaCl (pH 6.6), but most common biological buffers are suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Functional insights from recombinant production of bacterial proteases in Saccharomyces cerevisiae., PMID:40405239

Gel Electrophoretic Detection of Black Market ACE-031., PMID:40312924

Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from injection of crude sample - Incorporation of middle-up and bottom-up workflows., PMID:40221213

ImmunoPET imaging of Trop2 expression in triple-negative breast cancer using [64Cu]Cu-NOTA-Trodelvy-F(ab')2., PMID:39994021

Comparison of pharyngeal and invasive isolates of Streptococcus pyogenes by whole-genome sequencing in Toronto, Canada., PMID:39945517

Filtering through AAV capsid libraries for effective kidney gene transfer., PMID:39814263

Half-Life Extension of the IgG-Degrading Enzyme (IdeS) Using Fc-Fusion Technology., PMID:39703106

Insulin-Degrading Enzyme Efficiently Degrades polyQ Peptides but not Expanded polyQ Huntingtin Fragments., PMID:38640164

Impact of imlifidase treatment on immunoglobulins in an HLA-hypersensitized lupus nephritis patient with anti-SSA/SSB antibodies after kidney transplantation: A case report., PMID:38162455

Identification of bacterial protease domains that cleave human IgM., PMID:38061198

Pathogen-driven degradation of endogenous and therapeutic antibodies during streptococcal infections., PMID:37872209

IdeS, a secreted proteinase of Streptococcus pyogenes, is bound to a nuclease at the bacterial surface where it inactivates opsonizing IgG antibodies., PMID:37838172

Recombinant Endopeptidases IdeS and IdeZ and Their Potential Application., PMID:37748870

Modulation of the Activity of the Insulin-Degrading Enzyme by Aβ Peptides., PMID:37498802

Current Trends in MOF (Metal-Organic Framework) and Metal X-ides., PMID:37446366

Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae., PMID:37258965

Revealing charge heterogeneity of stressed trastuzumab at the subunit level., PMID:36693954

Extensive substrate recognition by the streptococcal antibody-degrading enzymes IdeS and EndoS., PMID:36528711

Using Digestion by IdeS Protease to Improve Quantification of Degradants in Monoclonal Antibodies by Non-Reducing Capillary Gel Electrophoresis., PMID:36472948

New approach to desensitization in solid organ transplantation-imlifidase., PMID:38994389

Altered Surface Expression of Insulin-Degrading Enzyme on Monocytes and Lymphocytes from COVID-19 Patients Both at Diagnosis and after Hospital Discharge., PMID:36232381

Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F(ab')2-Based SPECT/CT Imaging., PMID:35903247

Anchoring IgG-degrading enzymes to the surface of platelets selectively neutralizes antiplatelet antibodies., PMID:35737875

Multi-approach LC-MS methods for the characterization of species-specific attributes of monoclonal antibodies from plants., PMID:35550279

Insulin-Degrading Enzyme, an Under-Estimated Potential Target to Treat Cancer?, PMID:35406791

124I-Labeled Monoclonal Antibody and Fragment for the Noninvasive Evaluation of Tumor PD-L1 Expression In Vivo., PMID:35244407

Monolithic Papain-Immobilized Enzyme Reactors for Automated Structural Characterization of Monoclonal Antibodies., PMID:34859053

Elevated Fab glycosylation of anti-hinge antibodies., PMID:34726124

nES-DMA with Charge-reduction based on Soft X-ray Radiation: Analysis of a Recombinant Monoclonal Antibody., PMID:34543886

Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α., PMID:34401927

Incorporation of bacterial immunoevasins to protect cell therapies from host antibody-mediated immune rejection., PMID:34217891

Degradation of Alzheimer's Amyloid-β by a Catalytically Inactive Insulin-Degrading Enzyme., PMID:33865867

From virus to diabetes therapy: Characterization of a specific insulin-degrading enzyme inhibitor for diabetes treatment., PMID:33835493

Microdroplet Ultrafast Reactions Speed Antibody Characterization., PMID:33590747

Modulation of Insulin Sensitivity by Insulin-Degrading Enzyme., PMID:33477364

Treatment outcomes of integrase inhibitors, boosted protease inhibitors and nonnucleoside reverse transcriptase inhibitors in antiretroviral-naïve persons starting treatment., PMID:32588958

IgG-cleaving endopeptidase enables in vivo gene therapy in the presence of anti-AAV neutralizing antibodies., PMID:32483358

Production of F(ab')2 from Monoclonal and Polyclonal Antibodies., PMID:32319727

Intact and middle-down CIEF of commercial therapeutic monoclonal antibody products under non-denaturing conditions., PMID:32250465

The Proteolytic Cleavage of Therapeutic Monoclonal Antibody Hinge Region: More Than a Matter of Subclass., PMID:32117299

Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry., PMID:31838283

Drug Loading and Distribution of ADCs After Reduction or IdeS Digestion and Reduction., PMID:31643057

Pancreatic β-cell-specific deletion of insulin-degrading enzyme leads to dysregulated insulin secretion and β-cell functional immaturity., PMID:31479304

Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme., PMID:31086331

Effect of Fc Fusion on Folding and Immunogenicity of Middle East Respiratory Syndrome Coronavirus Spike Protein., PMID:30982320

Cleavage of anti-PF4/heparin IgG by a bacterial protease and potential benefit in heparin-induced thrombocytopenia., PMID:30917957

Middle-down approach: a choice to sequence and characterize proteins/proteomes by mass spectrometry., PMID:35521579

Administration of immunoglobulin G-degrading enzyme of Streptococcus pyogenes (IdeS) for persistent anti-ADAMTS13 antibodies in patients with thrombotic thrombocytopenic purpura in clinical remission., PMID:30488420

Enzyme kinetics from circular dichroism of insulin reveals mechanistic insights into the regulation of insulin-degrading enzyme., PMID:30305381

Tolerability of Current Antiretroviral Single-Tablet Regimens., PMID:30264826

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