Catalog No.
EBF78301
Description
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
Biological activity
At 25 ° C, reaction buffer 50 mM Tris-HCl (PH 8.0), the amount of enzyme required to digest 50 ug fusion protein containing the digestion site within 16 hours is defined as 1 unit of enzyme activity, which is 1U.
Expression system
Yeast
Species
Bovine
Protein length
Enterokinase is cloned from bovine and expressed in Yeast.
Predicted molecular weight
22.11 kDa
Nature
Recombinant
Target
Enterokinase
Concentration
> 30 KU / mg, 1mg of recombinant bovine enterokinase can cut at least 1.5g fusion protein.
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Applications
Fusion Protein Cleavage,Protein Purification,Protein Digestion
Form
Liquid
Storage buffer
50 mM Tris-HCl, 50%Glycerol, pH 8.0
Experimental Procedure
Digestion reaction condition
Digestion at 25 ℃ for 16 hours
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Optimization of enterokinase secretion in Pichia pastoris,PMID: 32924367
The trail of my studies on glycoproteins from enterokinase to tumor markers,PMID: 20551595
Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains,PMID: 8052624
Effects of mutations on MUC1-C/ED protein stability and antibody Binding: Structural insight., PMID:40499497
Role of CCK1 receptor in metabolic benefits of intestinal enteropeptidase inhibition in mice., PMID:40460092
Negatively Charged Peptides Assisted Gold Nanorod-Based Nano-Magic Bullet for Mitochondria-Targeting and Imaging-Guided Synergistic Photothermal/Dynamic Therapy., PMID:40454907
Functional Analysis of Mature Activin A Produced by Enterokinase in Plant Cells., PMID:40082306
Enterokinase deficiency associated with novel TMPRSS15 gene mutations: a case report., PMID:39944319
Critical Roles of SEA Domains., PMID:39930241
Production of recombinant masu salmon insulin-like growth factor binding protein-2b1 and its action on pituitary cells., PMID:39914704
A novel method for expressing and purifying large quantities of functional and stable human voltage-gated proton channel (hHv1)., PMID:39865375
Optimizing proinsulin production in E. coli BL21 (DE3) using taguchi method and efficient one-step insulin purification by on-column enzymatic cleavage., PMID:39847212
Engineering a high-throughput clone for industrial-scale production of long-acting GLP-1 analogue with retained bio-efficacy., PMID:39846515
Recombinant Expression of a New Antimicrobial Peptide Composed of hBD-3 and hBD-4 in Escherichia coli and Investigation of Its Activity Against Multidrug-Resistant Bacteria., PMID:39825027
Cleaving the way for heterologous peptide production: An overview of cleavage strategies., PMID:39638163
Oriental covalent immobilization of N-glycan binding protein via N-terminal selective modification., PMID:39489947
Production of active human iduronate-2-sulfatase (IDS) enzyme in Nicotiana benthamiana., PMID:39367006
Development of an Ultrasensitive Competitive Double-Enzyme Cascade Fluorescence Signal Amplification Method for Factor XIa Inhibitor Screening., PMID:39340422
Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycan., PMID:39305901
Intein Based Fusion Proteins: Great Tags for the Soluble Production and Convenient Purification of Recombinant Proteins., PMID:39220337
Insight into the Probiogenomic Potential of Enterococcus faecium BGPAS1-3 and Application of a Potent Thermostable Bacteriocin., PMID:39200563
Glycine-replaced epinecidin-1 variant bestows better stability and stronger antimicrobial activity against a range of nosocomial pathogenic bacteria., PMID:39034467
Preparation of recombinant neuritin protein., PMID:39002828
Enhancing immunogenicity and antiviral protection of inactivated porcine reproductive and respiratory syndrome virus vaccine in piglets., PMID:38889741
In-silico functional analyses identify TMPRSS15-mediated intestinal absorption of lithium as a modulator of lithium response in bipolar disorder., PMID:38735581
Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes., PMID:38676260
Structural Basis for Multivalent MUC16 Recognition and Robust Anti-Pancreatic Cancer Activity of Humanized Antibody AR9.6., PMID:38394685
Production of Mature Recombinant Human Activin A in Transgenic Rice Cell Suspension Culture., PMID:38392192
Machine Learning Integrating Protein Structure, Sequence, and Dynamics to Predict the Enzyme Activity of Bovine Enterokinase Variants., PMID:38386417
High-Yield Preparation of American Oyster Defensin (AOD) via a Small and Acidic Fusion Tag and Its Functional Characterization., PMID:38276646
Heterologous expression of the novel dimeric antimicrobial peptide LIG in Pichia pastoris., PMID:38181981
A D-Y Shaped Neuropeptide Y Mimetic Peptide-Dye Self-Assembly with Maximal Emission Beyond 1300 nm and Glioma Mitochondrial Activity Modulation., PMID:38109130
The molecular structure and function of fibrocystin, the key gene product implicated in autosomal recessive polycystic kidney disease (ARPKD)., PMID:37905714
Metformin inhibits digestive proteases and impairs protein digestion in mice., PMID:37863262
A novel approach to enhance the performance of kallikrein 6 enzyme using Pichia pastoris GS115 as a host., PMID:37842513
Microbial dietary protein metabolism regulates GLP-1 mediated intestinal transit., PMID:37732618
A comparison of digestive strategies for fishes with different feeding habits: Digestive enzyme activities, intestinal morphology, and gut microbiota., PMID:37706163
Discovery of a novel series of medium-sized cyclic enteropeptidase inhibitors., PMID:37683572
Impact of stabilizing mutations on the antigenic profile and glycosylation of membrane-expressed HIV-1 envelope glycoprotein., PMID:37549185
Functional expression of an antimicrobial peptide, belonging to halocin C8 family, from Natrinema sp. RNS21 in Escherichia coli., PMID:37548679
Effect of tandem repeats of antimicrobial peptide CC34 on production of target proteins and activity of Pichia pastoris., PMID:37536580
Characterization of digestive proteases in the gut of a basal deuterostome., PMID:37470128
Establishment and characterization of an immortalized bovine intestinal epithelial cell line., PMID:37351870
Efficient production of fluorophore-labeled CC chemokines for biophysical studies using recombinant enterokinase and recombinant sortase., PMID:37341434
Exocrine Pancreatic Insufficiency Is Common in Celiac Disease: A Systematic Review and Meta-Analysis., PMID:37294459
Complete Genome Sequencing and Bacteriocin Functional Characterization of Pediococcus ethanolidurans CP201 from Daqu., PMID:37285000
Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli., PMID:37170276
High yield expression in Pichia pastoris of human neutrophil elastase fused to cytochrome B5., PMID:36822453
An Exploratory Randomized Trial of SCO-792, an Enteropeptidase Inhibitor, in Patients With Type 2 Diabetes and Albuminuria., PMID:36644351
Production of active Exendin-4 in Nicotiana benthamiana and its application in treatment of type-2 diabetics., PMID:36618620
[Molecular tandem repeat strategy for production ofultrashort peptides]., PMID:36593195
A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli., PMID:36574134
Enterokinase deficiency with novel TMPRSS15 gene mutation masquerading as acrodermatitis enteropathica., PMID:36410965
For research use only. Not for human or drug use.
