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Anti-HRSV F/Fusion glycoprotein F0 Antibody (Am14)

Catalog #:   RVV02801 Specific References (8) DATASHEET
Host species: Human
Isotype: IgG1
Applications: ELISA, IHC, WB
Expression system: Mammalian Cells
Overview

Catalog No.

RVV02801

Expression system

Mammalian Cells

Species reactivity

HRSV-A

Host species

Human

Isotype

IgG1

Clonality

Monoclonal

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:5000

Target

F,Fusion glycoprotein F0

Concentration

1.48 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P03420

Form

Liquid

Storage buffer

100 mM Pro-Ac, 20 mM Arg, pH 5.0

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Am14

Note

For research use only.

Data Image
  • SDS-PAGE
    SDS PAGE for F / Fusion glycoprotein F0
  • Bioactivity
    Detects F/Fusion glycoprotein F0 in indirect ELISAs.
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
  • Bioactivity
    Detects HRSV F/Fusion glycoprotein F0 Protein in indirect ELISA.
  • Flow Cytometry
    The percentage of cells transfected with the rAAV5-RSV-F and rAAV5-RSV-Fm vaccines that expressed membrane-associated forms of the RSV F protein bound by D25, 4D7, or AM14 was determined (A, B). (n=3) Statistical significance was calculated via two-way ANOVA with Dunnett's multiple comparisons. P values were adjusted for multiple comparisons. **p < 0.01, ***p < 0.001.
    Data sourced from citation (PMID: 39469716)
  • Bioactivity
    The nanomolar affinities between each antigen and Nirsevimab, as well as AM14-1, confirm their correct conformations, reflecting their varying degrees of immunogenicity.The "pre-fusogenic" BR30 prepared according to the ResVax (Novavax) methodology (GenBank: QEI22738.1), was included for comparison purpose.Annotations:* : Indicates single-chain mutation variants. # : Denotes mutants containing S46G, S215P, and K465Q.
    Data sourced from citation (PMID: 39874917)
  • Bioactivity
    BLI profiles of BR38 (A), BR40 (B), and BR42 (C). Each column depicts interactions with Nirsevimab, D25, AM14-1, AM14-2, MPE8, and Palivizumab from top to bottom.
    Data sourced from citation (PMID: 39874917)
  • Bioactivity
    The table presents affinity coefficients of BR38, BR40, and BR42 with various mAbs. BR40 shows superior antigenicity, while BR42 demonstrates comparable performance. BR38 exhibits inferior characteristics, notably decreased affinity to AM14.Annotations:# : Denotes mutants containing S46G, A149C-Y458C, S215P and K465Q.
    Data sourced from citation (PMID: 39874917)
  • Bioactivity
    Long-term stability evaluation. (A-B) Long-term stability assessment of vaccine bulk solutions stored at 4 ℃, evaluated using (A) Nirsevimab and (B) AM14-1. BR40 and BR42 maintained higher levels of neutralizing epitope activity compared to Standard-1 and BR38, indicating superior long-term stability.
    Data sourced from citation (PMID: 39874917)
References

A Self-Assembled RSV-DS2 Based Nanovaccine Elicits a Strong Immune Response Against Respiratory Syncytial Virus Infection. [RVV02801]

Enhanced pulmonary histopathology is observed in cotton rats immunized with formalin-inactivated respiratory syncytial virus (RSV) or purified F glycoprotein and challenged with RSV 3-6 months after immunization., PMID:2251875

Intracellular processing of the human respiratory syncytial virus fusion glycoprotein: amino acid substitutions affecting folding, transport and cleavage., PMID:1375280

Membrane-associated respiratory syncytial virus F protein expressed from a human rhinovirus type 14 vector is immunogenic., PMID:11277694

Prefusion F, Postfusion F, G Antibodies, and Disease Severity in Infants and Young Children With Acute Respiratory Syncytial Virus Infection., PMID:29029312

Dissociation of the respiratory syncytial virus F protein-specific human IgG, IgA and IgM response., PMID:33574352

Development of a genetically modified full-length human respiratory syncytial virus preF protein vaccine. [RVV02801]

Induction of neutralizing antibody responses by AAV5-based vaccine for respiratory syncytial virus in mice. [RVV02801]

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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For research use only. Not for human or drug use.

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Anti-HRSV F/Fusion glycoprotein F0 Antibody (Am14) [RVV02801]
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