Anti-6*His Tag (HHHHHH) Antibody (3D5)

Catalog No.

RGK07901

Host species

Rabbit

Isotype

IgG1, kappa

Clonality

Monoclonal

Target

6*His Tag (HHHHHH), 6HIS, 6*HIS,6xHis

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

IF, IP, WB

Form

Liquid

Storage buffer

PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

3D5

Rational design and pairing of single-domain antibodies for developing rapid detection methods for botulinum neurotoxins A and B., PMID:40253065

Cloning and Recombinant Expression of the Caspase-Activated DNase Orthologous Gene of Giardia lamblia., PMID:40224544

Intranasal Immunization with Nasal Immuno-Inducible Sequence-Fused Antigens Elicits Antigen-Specific Antibody Production., PMID:39684539

Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis., PMID:38782081

Production of Plant-Derived Japanese Encephalitis Virus Multi-Epitope Peptide in Nicotiana benthamiana and Immunological Response in Mice., PMID:37511402

Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein., PMID:35916768

Development and evaluation of indirect enzyme-linked immunosorbent assay using recombinant dense granule antigen 7 protein for the detection of Toxoplasma gondii infection in cats in Thailand., PMID:35497967

Screening and identification of B cell epitope of the nucleocapsid protein in SARS-CoV-2 using the monoclonal antibodies., PMID:35037999

A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli., PMID:38650268

Purification of Recombinant Mouse C-Reactive Protein from Pichia Pastoris GS115 by Nickel Chelating Sepharose Fast-Flow Affinity Chromatography and P-Aminophenyl Phosphoryl Choline Agarose Resin Affinity Chromatography in Tandem., PMID:34625786

Functional recombinant human complement C1q with different affinity tags., PMID:33621564

Efficient application of a baculovirus-silkworm larvae expression system for obtaining porcine circovirus type 2 virus-like particles for a vaccine., PMID:32757056

Dual in vitro invasion/migration suppressing and tamoxifen response modulating effects of a recombinant anti-ALCAM scFv on breast cancer cells., PMID:32196701

Robust Bioengineered Apoferritin Nanoprobes for Ultrasensitive Detection of Infectious Pancreatic Necrosis Virus., PMID:30938982

Production and characterization of recombinant P1 adhesin essential for adhesion, gliding, and antigenic variation in the human pathogenic bacterium, Mycoplasma pneumoniae., PMID:30551878

Expression of a functional intrabody against hepatitis C virus core protein in Escherichia coli and silkworm pupae., PMID:29778543

Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis., PMID:29736150

[Expression, purification and immunoreactivity characterization of extracellular antigenic domains of NMDAR1 protein]., PMID:29271175

Generation and characterization of a potentially applicable Vero cell line constitutively expressing the Schmallenberg virus nucleocapsid protein., PMID:28083834

[Preparation of polyclonal antibody against recombinant sigma54 protein from Helicobacter pylori]., PMID:28031123

Immuno-detection of dioxins using a recombinant protein of aryl hydrocarbon receptor (AhR) fused with sfGFP., PMID:27328714

Adhesion properties of a putative polymorphic fimbrial subunit protein from Bifidobacterium longum subsp. longum., PMID:26858927

Heterologous Expression of Hepatitis C Virus Core Protein in Oil Seeds of Brassica napus L., PMID:26855744

APC targeting enhances immunogenicity of a novel multistage Fc-fusion tuberculosis vaccine in mice., PMID:26373723

Cloning and characterization of a novel β-ketoacyl-ACP reductase from Comamonas testosteroni., PMID:25595225

Expression, purification of IL-38 in Escherichia coli and production of polyclonal antibodies., PMID:25448591

[Effect of α -1,2 fucosyltransferase gene 682A> G and 547_552delAG mutations on the activity of fucosyltransferase]., PMID:25297588

Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli., PMID:24215864

Cloning, expression and N-terminal formylation of ESAT-6 of Mycobacterium tuberculosis H37Rv., PMID:24056255

Dependence of nanoparticle-cell recognition efficiency on the surface orientation of scFv targeting ligands., PMID:32481827

Pichia pastoris-expressed dengue 2 envelope forms virus-like particles without pre-membrane protein and induces high titer neutralizing antibodies., PMID:23717637

An antigenic domain of the Leishmania amazonensis nucleoside triphosphate diphosphohydrolase (NTPDase 1) is associated with disease progression in susceptible infected mice., PMID:23681191

Design, production, and characterization of a single-chain variable fragment (ScFv) derived from the prostate specific membrane antigen (PSMA) monoclonal antibody J591., PMID:23500147

Immunological features and efficacy of the reconstructed epitope vaccine CtUBE against Helicobacter pylori infection in BALB/c mice model., PMID:23064457

Functional recombinant extra membrane loop of human CD20, an alternative of the full length CD20 antigen., PMID:23023212

Serodiagnosis efficacy and immunogenicity of the fusion protein of Mycobacterium tuberculosis composed of the 10-kilodalton culture filtrate protein, ESAT-6, and the extracellular domain fragment of PPE68., PMID:22357648

Cloning, soluble expression, rapid purification and characterization of human Cofilin1., PMID:22251882

Structural and functional properties of NH(2)-terminal domain, core domain, and COOH-terminal extension of αA- and αB-crystallins., PMID:21921988

Highly efficient production of anti-HER2 scFv antibody variant for targeting breast cancer cells., PMID:21538107

Production and characterization of LEA29Y, a variant of cytotoxic T-lymphocyte antigen 4-immunoglobulin, in Pichia pastoris., PMID:21533579

Influence of v5/6-His tag on the properties of gap junction channels composed of connexin43, connexin40 or connexin45., PMID:21424225

Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein., PMID:21148192

[Preparation, characterization and application of rice Qb-SNARE protein OsNPSN11 polyclonal antibody]., PMID:20870618

EspA is a novel fusion partner for expression of foreign proteins in Escherichia coli., PMID:20854850

[Cloning, expression and functional analysis of the dhbC gene from the siderophore producing bacterium Bacillus subtilis CAS15]., PMID:19777807

Neutralization of white spot syndrome virus (WSSV) for Penaeus chinensis by antiserum raised against recombinant VP19., PMID:19195390

[Cloning, expression and purification of allergen arginine kinase from Periplaneta americana and its allergic activity]., PMID:19157299

[Preparation of monoclonal antibody against human hNudC and its expression in human megakaryocytes]., PMID:20108442

[Expression and purification of T7 bacteriophage capsid protein P11 and preparation of monoclonal antibody against P11]., PMID:18466708

[Cloning, expression and purification of dust mite allergen Der f 3 and identification of its allergic activity]., PMID:17639694

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