Catalog No.
RHF34001
Description
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.
Expression system
Mammalian Cells
Species reactivity
Human
Host species
Human
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000
Target
CD81 antigen,TSPAN28,Target of the antiproliferative antibody 1,Tspan-28,Tetraspanin-28,CD81,TAPA1,26 kDa cell surface protein TAPA-1
Concentration
1.03 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P60033
Applications
ELISA, FCM, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
005-C01
Glycoengineering of the hepatitis C virus E2 glycoprotein improves biochemical properties and enhances immunogenicity., PMID:40500295
Ultrasound-triggered targeted delivery of engineered ADSCs-derived exosomes with high SDF-1α levels to promote cardiac repair following myocardial infarction., PMID:40460966
An Extracellular Vesicle (EV) Paper Strip for Rapid and Convenient Estimation of EV Concentration., PMID:40422033
Development of a high-sensitivity assay for direct quantitation of EGFr on extracellular vesicles in plasma., PMID:40345522
[Efficient capture and proteomics analysis of urinary extracellular vesicles by affinity purification]., PMID:40331614
Extracellular Vesicle Marker Changes Associated With Disease Activity in Relapsing-Remitting Multiple Sclerosis., PMID:40300121
Glycoengineering of the hepatitis C virus E2 glycoprotein leads to improved biochemical properties and enhanced immunogenicity., PMID:40291659
Microfluidic Nano-Plasmonic Imaging Platform for Purification- and Label-Free Single Small Extracellular Vesicle Counting., PMID:40166220
Nanoscopic Profiling of Small Extracellular Vesicles via High-Speed Atomic Force Microscopy (HS-AFM) Videography., PMID:40139685
Next Generation Aqueous Two-Phase System for Gentle, Effective, and Timely Extracellular Vesicle Isolation and Transcriptomic Analysis., PMID:40108918
Isolation of Urinary Extracellular Vesicles (EVs) via Hydrophobic Interaction Chromatography Using a Nylon-6 Capillary-Channeled Polymer (C-CP) Fiber Column., PMID:39933961
EV-Lev: extracellular vesicle isolation from human plasma using microfluidic magnetic levitation device., PMID:39918033
Tetraspanin CD81 serves as a functional entry factor for porcine circovirus type 2 infection., PMID:39745447
EV-Elute: A universal platform for the enrichment of functional surface marker-defined extracellular vesicle subpopulations., PMID:39692115
A novel multiple marker microarray analyzer and methodology to predict major obstetric syndromes using surface markers of circulating extracellular vesicles from maternal plasma., PMID:39607297
Streamlined miRNA loading of surface protein-specific extracellular vesicle subpopulations through electroporation., PMID:39574085
Multi-omic characteristics of longitudinal immune profiling after breakthrough infections caused by Omicron BA.5 sublineages., PMID:39536392
Investigating the effect of immunomagnetic separation on the immunophenotype and viability of plasma cells in plasma cell disorders., PMID:39493694
Citrullination Accompanies the Development of Carotid Atherosclerotic Plaques., PMID:39421992
Comparison of nanoimaging and nanoflow based detection of extracellular vesicles at a single particle resolution., PMID:39416671
Enhancing Extracellular Vesicle Detection via Cotargeting Tetraspanin Biomarkers., PMID:39360503
Multiparametric profiling of HER2-enriched extracellular vesicles in breast cancer using Single Extracellular VEsicle Nanoscopy., PMID:39342336
Sensitive phenotyping of serum extracellular vesicles on a SERS-microfluidic platform for early-stage clinical diagnosis of ovarian carcinoma., PMID:39260102
Pharmacologic HIF stabilization activates costimulatory receptor expression to increase antitumor efficacy of adoptive T cell therapy., PMID:39196939
Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response., PMID:39176090
Hepatitis C virus chronicity and oncogenic potential: Vaccine development progress., PMID:39167987
CD81 fusion alters SARS-CoV-2 Spike trafficking., PMID:39140770
Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer., PMID:39140467
Urinary Extracellular Vesicles as a Readily Available Biomarker Source: A Simplified Stratification Method., PMID:39125575
Exosomal membrane proteins analysis using a silicon nanowire field effect transistor biosensor., PMID:39002259
Immunoassays for Extracellular Vesicle Detection via Transmembrane Proteins Using Surface Plasmon Resonance Biosensors., PMID:38912608
Circulating small extracellular vesicles in Alzheimer's disease: a case-control study of neuro-inflammation and synaptic dysfunction., PMID:38902659
Isolation and quantification of L1CAM-positive extracellular vesicles on a chip as a potential biomarker for Parkinson's Disease., PMID:38898558
Fine-tuning spatial-temporal dynamics and surface receptor expression support plasma cell-intrinsic longevity., PMID:38896451
Impact of brain organoid-derived sEVs on metastatic adaptation and invasion of breast carcinoma cells through a microphysiological system., PMID:38888211
Computational Study on the Wealth Conformations of Hepatitis C Virus Envelope Glycoprotein 2 and Role of Its Glycan Coat., PMID:38861660
Alterations in B and NK cells highly correlate with disease severity in children with COVID-19., PMID:38813014
Immune checkpoint inhibitors for POLE or POLD1 proofreading-deficient metastatic colorectal cancer., PMID:38777726
High-throughput proteomic analysis of extracellular vesicles from saliva by chemical probe-based array., PMID:38772652
The antitumor effect of extracellular vesicles derived from cytokine-activated CD8+ T cells., PMID:38753658
CD71: Role in permafrost immunity., PMID:38711995
Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed., PMID:38659349
Spectroscopic insight into breast cancer: profiling small extracellular vesicles lipids via infrared spectroscopy for diagnostic precision., PMID:38654096
[Exosomes' role in intercellular interactions in different variants of lung injury in fatal cases of COVID-19]., PMID:38591903
A molecular docking exploration of the large extracellular loop of tetraspanin CD81 with small molecules., PMID:38584777
Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes., PMID:38520356
Isoprinosine as a foot-and-mouth disease vaccine adjuvant elicits robust host defense against viral infection through immunomodulation., PMID:38510965
Faster clinical decisions in B-cell acute lymphoblastic leukaemia: A single flow cytometric 12-colour tube improves diagnosis and minimal residual disease follow-up., PMID:38432068
Decoding of the surfaceome and endocytome in primary glioblastoma cells identifies potential target antigens in the hypoxic tumor niche., PMID:38414005
Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker., PMID:38400284
For research use only. Not for human or drug use.
