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Anti-Human FGF19 Antibody (SAA1435) (RHB73301)

Host species:Human
Isotype:IgG1
Applications:ELISA, FCM, IHC, WB
Expression system:Mammalian Cells
Overview

Catalog No.

RHB73301

Expression system

Mammalian Cells

Species reactivity

Human

Host species

Human

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:500-1:4000

Target

FGF19,Fibroblast growth factor 19,FGF-19

Concentration

0.84 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O95750

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA1435

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human FGF19 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF19 antibody monoclonal antibody (Catalog # RHB73301 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with FGF19 antibody (RHB73301) at 1 µg/ml.

    Lane 1: Recombinant Human FGF19 Protein

    Second Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
References
Datasheet
$ 328
Product specifications
100 μg 328 1 mg 1640

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human FGF19 Antibody (SAA1435) [RHB73301]
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