Catalog No.
YVV01702
Expression system
E. coli
Species
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein length
Asp4891-Val5212
Predicted molecular weight
64 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P0DTD1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
RNA-directed RNA polymerase, Pol, RdRp, Non-structural protein 12, nsp12, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein
Genome-Wide Interrogation of SARS-CoV-2 RNA-Protein Interactions Uncovers Hidden Regulatory Sites., PMID:40501555
Unveiling SARS-CoV-2's heart: role, structure and inhibition of SARS-CoV-2 RNA-dependent RNA polymerase., PMID:40473220
Single-Molecule Assay Reveals Binding Dynamics of SARS-CoV-2 Polymerase Components and Provides a New Tool to Distinguish Polymerase Inhibitors., PMID:40465830
A post-assembly conformational change makes the SARS-CoV-2 polymerase elongation-competent., PMID:40464687
Mechanistic insights into the activity of SARS-CoV-2 RNA polymerase inhibitors using single-molecule FRET., PMID:40298114
SARS-CoV-2 biological clones are genetically heterogeneous and include clade-discordant residues., PMID:40272156
In Silico and In Vitro development of novel small interfering RNAs (siRNAs) to inhibit SARS-CoV-2., PMID:40256168
Clinical and molecular landscape of prolonged SARS-CoV-2 infection with resistance to remdesivir in immunocompromised patients., PMID:40160532
In silico studies to understand the interactions of flavonoid inhibitor with nsp12-RNA dependent RNA polymerase of SARS-CoV-2 and its homologs., PMID:40160515
A novel replicase-mediated self-amplifying RNA amplification mechanism of the SARS-CoV-2 replication-transcription system., PMID:40117978
Drupacine as a potent SARS-CoV-2 replication inhibitor in vitro., PMID:40078736
Covalent inhibition of the SARS-CoV-2 NiRAN domain via an active-site cysteine., PMID:40049411
Evaluation of Genomic Surveillance of SARS-CoV-2 Virus Isolates and Comparison of Mutational Spectrum of Variants in Bangladesh., PMID:40006937
Remdesivir and Obeldesivir Retain Potent Antiviral Activity Against SARS-CoV-2 Omicron Variants., PMID:40006923
A comprehensive review of current insights into the virulence factors of SARS-CoV-2., PMID:39878471
Investigation of Immunoreactivity Profiles and Epitope Landscape in Divergent COVID-19 Trajectories and SARS-CoV-2 Variants., PMID:39873496
The impact of remdesivir on SARS-CoV-2 evolution in vivo., PMID:39836474
A post-assembly conformational change makes the SARS-CoV-2 polymerase elongation-competent., PMID:39829827
Single-molecule assay reveals the impact of composition, RNA duplex, and inhibitors on the binding dynamics of SARS-CoV-2 polymerase complex., PMID:39829757
Discovery of a 2'-α-Fluoro-2'-β-C-(fluoromethyl) Purine Nucleotide Prodrug as a Potential Oral Anti-SARS-CoV-2 Agent., PMID:39804580
SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12., PMID:39737877
CRTC3 restricts SARS-CoV-2 replication and is antagonized by CREB., PMID:39736320
SARS-CoV-2 resistance analyses from the Phase 3 PINETREE study of remdesivir treatment in nonhospitalized participants., PMID:39699245
Covalent DNA-Encoded Library Workflow Drives Discovery of SARS-CoV-2 Nonstructural Protein Inhibitors., PMID:39574309
Phylogeogenomic analysis of the earliest reported sequences of SARS-CoV-2 from 161 countries., PMID:39563179
Complete genome sequencing of SARS-CoV-2 strains that were circulating in Uzbekistan over the course of four pandemic waves., PMID:39561193
SARS-CoV-2 Resistance to Small Molecule Inhibitors., PMID:39559548
The effects of Remdesivir's functional groups on its antiviral potency and resistance against the SARS-CoV-2 polymerase., PMID:39510431
Engineered protein destabilization reverses intrinsic immune evasion for candidate vaccine pan-strain KSHV and SARS-CoV-2 antigens., PMID:39484438
MDA5 Is a Major Determinant of Developing Symptoms in Critically Ill COVID-19 Patients., PMID:39460899
Higher Frequency of SARS-CoV-2 RNA Shedding by Cats than Dogs in Households with Owners Recently Diagnosed with COVID-19., PMID:39459932
Biochemical simulation of mutation synthesis and repair during SARS-CoV-2 RNA polymerization., PMID:39366027
Characterization of the Conformational Hotspots of the RNA-Dependent RNA Polymerase Complex Identifies a Unique Structural Malleability of nsp8., PMID:39356135
A genus-specific nsp12 region impacts polymerase assembly in Alphacoronavirus and Gammacoronavirus., PMID:39307300
Emergence of transmissible SARS-CoV-2 variants with decreased sensitivity to antivirals in immunocompromised patients with persistent infections., PMID:39294134
Characterization of ACTN4 as a novel antiviral target against SARS-CoV-2., PMID:39289355
Assessing the inhibitory effects of some secondary amines, thioureas and 1,3-dimethyluracil conjugates of (-)-cytisine and thermopsine on the RNA-dependent RNA polymerase of SARS-CoV-1 and SARS-CoV-2., PMID:39251111
Comparative Atlas of SARS-CoV-2 Substitution Mutations: A Focus on Iranian Strains Amidst Global Trends., PMID:39205305
Biochemical characterization of naturally occurring mutations in SARS-CoV-2 RNA-dependent RNA polymerase., PMID:39145418
Point mutations at specific sites of the nsp12-nsp8 interface dramatically affect the RNA polymerization activity of SARS-CoV-2., PMID:38990941
Mechanistic insights into bismuth(iii) inhibition of SARS-CoV-2 helicase., PMID:38966375
Emergence and fixation of SARS-CoV-2 minority variants in a chronically infected patient receiving therapy in Denmark., PMID:38961316
A Biochemical and Biophysical Analysis of the Interaction of nsp9 with nsp12 from SARS-CoV-2-Implications for Future Drug Discovery Efforts., PMID:38958516
Heterotypic immunity from prior SARS-CoV-2 infection but not COVID-19 vaccination associates with lower endemic coronavirus incidence., PMID:38865483
In silico analysis of non-structural protein 12 sequences from SARS-COV-2 found in Manaus, Amazonas, Brazil, reveals mutations linked to higher transmissibility., PMID:38747801
Dynamic diversity of SARS-CoV-2 genetic mutations in a lung transplantation patient with persistent COVID-19., PMID:38684722
Discovery of C-Linked Nucleoside Analogues with Antiviral Activity against SARS-CoV-2., PMID:38651692
SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters., PMID:38628304
Characteristics of SARS-CoV-2 Omicron BA.5 variants in Shanghai after ending the zero-COVID policy in December 2022: a clinical and genomic analysis., PMID:38605705
Optimized Recombinant Expression and Purification of the SARS-CoV-2 Polymerase Complex., PMID:38511495
For research use only. Not for human or drug use.
