Catalog No.
KVV09601
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for CHIKV E1 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any CHIKV E1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for CHIKV E1 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHIKV E1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of CHIKV E1 concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative (Sandwich)
Range
62.5 - 4,000 pg/mL
Sensitivity
51.23 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (pg/mL) |
1962.1 |
468.1 |
99.6 |
1918.0 |
470.9 |
110.0 |
|
Standard deviation |
73.0 |
14.1 |
8.6 |
60.7 |
25.4 |
14.1 |
|
CV (%) |
3.7 |
3.0 |
8.6 |
3.2 |
5.4 |
12.9 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
Spike glycoprotein E1, E1
For research use only. Not for human or drug use.
