Catalog No.
KAD10801
Description
This assay employs the quantitative sandwich enzyme immunoassay technique. Mouse FMC63 scFv has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-FMC63 scFv Antibody will be captured by immobilized Mouse FMC63 scFv. After washing away any unbound substances, a biotin-labeled Mouse FMC63 scFv is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-FMC63 scFv Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
1.56 - 100 ng/mL
Sensitivity
1.04 ng/mL
Precision
CV<15%
Recovery
80-120%
Stability and Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Alternative Names
Anti-FMC63 scFv antibody, CD19, CAR-T, Mouse FMC63 scFv
Background
FMC63 is a mouse IgG2a monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. Anti-FMC63 scFv antibody can specifically bind to the antigen recognition epitope of FMC63 scFv on anti-CD19 CAR, and it shows high specificity and sensitivity, it's used to detect the expression of FMC63 scFv derived CAR.
For research use only. Not for human or drug use.
