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Anti-USP7 Antibody (R3V69)

Catalog #:   RHJ35603 Specific References (27) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q93009
Overview

Catalog No.

RHJ35603

Species reactivity

Human, Mouse, Rat, Monkey

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Ubiquitin-specific-processing protease 7, Deubiquitinating enzyme 7, Ubiquitin thioesterase 7, USP7, Herpesvirus-associated ubiquitin-specific protease, HAUSP, Ubiquitin carboxyl-terminal hydrolase 7

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q93009

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3V69

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using USP7 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of HepG2 cells using USP7 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of Jurlat cells using USP7 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of K562 cells using USP7 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using mouse USP7 mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using mouse USP7 mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using USP7 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using USP7 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using USP7 mouse mAb against Hela (1), A431 (2), MCF-7 (3),Jurkat (4),K562 (5),HepG2 (6),A549 (7),HCT116 (8),HT-29 (9),SW480 (10),C6 (11),COS-7 (12),and NIH/3T3 (13) cell lysate.
References

USP7 accelerates colorectal cancer progression by up-regulating MYO6 through deubiquitination., PMID:40446734

Selective USP7 Inhibition Synergizes with MEK1/2 Inhibitor to Enhance Immune Responses and Potentiate Anti-PD-1 Therapy in NRAS-Mutant Melanoma., PMID:40204067

USP7 promotes IgA class switching through stabilizing RUNX3 for germline transcription activation., PMID:38735043

Cellular Assays for Dynamic Quantification of Deubiquitinase Activity and Inhibition., PMID:37858708

Isorhamnetin and anti-PD-L1 antibody dual-functional mesoporous silica nanoparticles improve tumor immune microenvironment and inhibit YY1-mediated tumor progression., PMID:37408047

ANXA1-derived peptide for targeting PD-L1 degradation inhibits tumor immune evasion in multiple cancers., PMID:37001908

A Designer Nanoparticle Platform for Controlled Intracellular Delivery of Bioactive Macromolecules: Inhibition of Ubiquitin-Specific Protease 7 in Breast Cancer Cells., PMID:35796308

Evaluation of In Vitro Tools to Predict the In Vivo Absorption of Biopharmaceuticals Following Subcutaneous Administration., PMID:35429492

Acetylated DNMT1 Downregulation and Related Regulatory Factors Influence Metastatic Melanoma Patients Survival., PMID:34572918

A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN., PMID:33348378

Pharmacological inhibition of USP7 promotes antitumor immunity and contributes to colon cancer therapy., PMID:30697058

Genome-wide discovery of somatic coding and noncoding mutations in pediatric endemic and sporadic Burkitt lymphoma., PMID:30617194

Prospero-related homeobox 1 drives angiogenesis of hepatocellular carcinoma through selectively activating interleukin-8 expression., PMID:28646551

Ubiquitin-specific Protease-7 Inhibition Impairs Tip60-dependent Foxp3+ T-regulatory Cell Function and Promotes Antitumor Immunity., PMID:27769803

STIP is a critical nuclear scaffolding protein linking USP7 to p53-Mdm2 pathway regulation., PMID:26460617

The Deubiquitinating Enzyme USP7 Regulates Androgen Receptor Activity by Modulating Its Binding to Chromatin., PMID:26175158

FBXW7 and USP7 regulate CCDC6 turnover during the cell cycle and affect cancer drugs susceptibility in NSCLC., PMID:25885523

Lung neuroendocrine tumors: correlation of ubiquitinylation and sumoylation with nucleo-cytosolic partitioning of PTEN., PMID:25884169

Annexin-1 regulated by HAUSP is essential for UV-induced damage response., PMID:25695607

A survey of the interactome of Kaposi's sarcoma-associated herpesvirus ORF45 revealed its binding to viral ORF33 and cellular USP7, resulting in stabilization of ORF33 that is required for production of progeny viruses., PMID:25694600

Genome-wide association study of antibody level response to NDV and IBV in Jinghai yellow chicken based on SLAF-seq technology., PMID:25588649

Quantitative Lys-ϵ-Gly-Gly (diGly) proteomics coupled with inducible RNAi reveals ubiquitin-mediated proteolysis of DNA damage-inducible transcript 4 (DDIT4) by the E3 ligase HUWE1., PMID:25147182

BCR-ABL disrupts PTEN nuclear-cytoplasmic shuttling through phosphorylation-dependent activation of HAUSP., PMID:24317448

Overexpression of the ubiquitin-specific protease 7 resulting from transfection or mutations in the ICP0 binding site accelerates rather than depresses herpes simplex virus 1 gene expression., PMID:22993145

M phase phosphorylation of the epigenetic regulator UHRF1 regulates its physical association with the deubiquitylase USP7 and stability., PMID:22411829

Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes., PMID:22118674

Affinity purification strategy to capture human endogenous proteasome complexes diversity and to identify proteasome-interacting proteins., PMID:19193609

Datasheet

Document Download

Anti-USP7 Antibody (R3V69).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-USP7 Antibody (R3V69) [RHJ35603]
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