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Anti-POU5F1/OCT3/OCT4 Antibody (R3Q45)

Catalog #:   RHF90001 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: WB
Accession: Q01860
Overview

Catalog No.

RHF90001

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

WB: 1:1000

Target

Octamer-binding protein 4, Oct-4, OCT3, OCT4, OTF3, OTF-3, Octamer-binding transcription factor 3, POU5F1, Oct-3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q01860

Applications

WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Q45

Data Image
  • Western blot
    Western blot analysis using OCT4 mouse mAb against PMA treated HepG2 cell lysate (1).
References

Pluripotent stem cell marker deficiency in salivary mucoepidermoid carcinoma with relevance to molecular profiling: An experimental study., PMID:40478494

A dual-Color closed bipolar electrochemiluminescence platform for visual simultaneous diagnosis of two pluripotency markers in cancer patients' urine., PMID:40253801

Myoepithelial tumors of soft tissue and bone in children and young adults: A clinicopathologic study of 40 cases occurring in patients ≤ 21 Years of age., PMID:38782103

Prostate cancer research: tools, cell types, and molecular targets., PMID:38595814

[Effect of Human Bone Marrow Mesenchymal Stem Cells with Ectopic High OCT4 Expression on T Lymphocyte Function]., PMID:37846711

Causal associations between prodromal infection and neuromyelitis optica spectrum disorder: A Mendelian randomization study., PMID:37540821

Analysis of N-glycosylation protein of Kashin-Beck disease chondrocytes derived from induced pluripotent stem cells based on label-free strategies with LC-MS/MS., PMID:37186116

Oct4 reduction contributes to testicular injury of unilateral testicular torsion in mice model and apoptotic death of Sertoli cells through mediating CIP2A expression., PMID:36690227

A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability., PMID:35975322

Transient DUX4 expression in human embryonic stem cells induces blastomere-like expression program that is marked by SLC34A2., PMID:35777358

POU5F1 Protein and Gene Expression Analysis in Neonate and Adult Mouse Testicular Germ Cells by Immunohistochemistry and Immunocytochemistry., PMID:34788058

Exon and intron sharing in opposite direction-an undocumented phenomenon in human genome-between Pou5f1 and Tcf19 genes., PMID:34610795

Targeted mass spectrometry for monitoring of neural differentiation., PMID:34357391

Expression of SSEA-4 and Oct-4 from somatic cells in primary mouse gastric cell culture induced by brief strong acid., PMID:33733429

Generation of Systemic Lupus Erythematosus Patient-Derived Induced Pluripotent Stem Cells from Blood., PMID:33397195

Breast tumour cell subpopulations with expression of the MYC and OCT4 proteins., PMID:33037978

Isolation, identification and differentiation of human spermatogonial cells on three-dimensional decellularized sheep testis., PMID:32992121

Posttranscriptional regulation of maternal Pou5f1/Oct4 during mouse oogenesis and early embryogenesis., PMID:32930837

Oct4 promotes M2 macrophage polarization through upregulation of macrophage colony-stimulating factor in lung cancer., PMID:32487125

MYC transcription activation mediated by OCT4 as a mechanism of resistance to 13-cisRA-mediated differentiation in neuroblastoma., PMID:32409685

Detection of circulating natural antibodies against CD25, MUC1, and VEGFR1 for early diagnosis of non-small cell lung cancer., PMID:32392378

Targeted sequencing reveals the mutational landscape responsible for sorafenib therapy in advanced hepatocellular carcinoma., PMID:32373219

OCT4 immunohistochemistry after staging laparoscopic retroperitoneal lymphadenectomy for testicular tumor., PMID:31819332

Humanized Stem Cell Models of Pediatric Medulloblastoma Reveal an Oct4/mTOR Axis that Promotes Malignancy., PMID:31786016

Mass cytometry-based single-cell analysis of human stem cell reprogramming uncovers differential regulation of specific pluripotency markers., PMID:31570522

Expression of Components of the Renin-Angiotensin System by the Embryonic Stem Cell-Like Population within Keloid Lesions., PMID:31348346

Targeting of replicating CD133 and OCT4/SOX2 expressing glioma stem cells selects a cell population that reinitiates tumors upon release of therapeutic pressure., PMID:31267022

Hypermethylation of microRNA-149 activates SDF-1/CXCR4 to promote osteogenic differentiation of mesenchymal stem cells., PMID:31206187

Differentiation of spermatogonial stem cells by soft agar three-dimensional culture system., PMID:31131631

Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome., PMID:30739749

Establishment and characterization of human theca stem cells and their differentiation into theca progenitor cells., PMID:30132968

Generation of Transplantable Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD)., PMID:29896658

Mapping transcription factor occupancy using minimal numbers of cells in vitro and in vivo., PMID:29572359

Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesis., PMID:29528446

[Ewing sarcomas and Ewing-like sarcomas : New aspects]., PMID:29480450

Effects of prostaglandin E2 on clonogenicity, proliferation and expression of pluripotent markers in human periodontal ligament cells., PMID:28763754

OCT4 controls mitotic stability and inactivates the RB tumor suppressor pathway to enhance ovarian cancer aggressiveness., PMID:28319064

The serine 106 residue within the N-terminal transactivation domain is crucial for Oct4 function in mice., PMID:28264735

Tissue organization alters gene expression in equine induced trophectoderm cells., PMID:28161437

New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells., PMID:28009074

Enhanced expression of the stemness-related factors OCT4, SOX15 and TWIST1 in ectopic endometrium of endometriosis patients., PMID:27881125

Identification of miRSNPs associated with the risk of multiple myeloma., PMID:27718532

Primary Mediastinal Yolk Sac Tumors: An Immunohistochemical Analysis of 14 Cases., PMID:27643524

Positive Oct -3/4 and D2-40 Immunohistochemical Expression in Germ Cells and Suspected Histology Pattern of Intratubular Germ Cell Neoplasia in Boys with Cryptorchidism Vanish after the Age of 2 Years., PMID:27606906

Arsenite inhibits the function of CD133+ CD13+ liver cancer stem cells by reducing PML and Oct4 protein expression., PMID:27517564

Characterization of the POU5F1 Homologue in Nile Tilapia: From Expression Pattern to Biological Activity., PMID:27473876

Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia., PMID:27129396

High-resolution telomere fluorescence in situ hybridization reveals intriguing anomalies in germ cell tumors., PMID:27085557

WNT/β-catenin and p27/FOXL2 differentially regulate supporting cell proliferation in the developing ovary., PMID:26939755

Tumor cell-intrinsic PD-L1 promotes tumor-initiating cell generation and functions in melanoma and ovarian cancer., PMID:28798885

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-POU5F1/OCT3/OCT4 Antibody (R3Q45) [RHF90001]
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