Anti-TRIM21/RO52 Antibody (R3F35)

Catalog No.

RHD34302

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

IHC: 1:50-1:200, WB: 1:500-1:1000

Target

SS-A, E3 ubiquitin-protein ligase TRIM21, TRIM21, Ro(SS-A), 52 kDa Ro protein, Tripartite motif-containing protein 21, RING finger protein 81, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RNF81, Sjoegren syndrome type A antigen, RO52, RING-type E3 ubiquitin transferase TRIM21, SSA1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P19474

Applications

IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3F35

Crystallographic fragment screening reveals ligand hotspots in TRIM21 PRY-SPRY domain., PMID:40514378

Anti-Ro52 Seropositive Interstitial Lung Disease is Associated with a Higher Risk for Disease Progression and Mortality., PMID:40484372

Mechanistic considerations linking SARS-CoV-2 infection, inflammation, and the loss of immune tolerance., PMID:40412200

Dual roles of CXCR4 (C-X-C motif chemokine receptor 4) in promoting entry of ebolavirus and targeting excessive glycoprotein for reticulophagic degradation to facilitate viral fitness., PMID:40223186

Potential Novel Tissue Biomarkers in Salivary Glands of Patients with Sjogren's Syndrome., PMID:40217840

Pulmonary Myeloid Cells in Mild Cases of COVID-19 Upregulate the Intracellular Fc Receptor TRIM21 and Transcribe Proteasome-Associated Molecules., PMID:40141410

RBMS3-loss impedes TRIM21-induced ubiquitination of ANGPT2 in an RNA-independent manner and drives sorafenib resistance in hepatocellular carcinoma., PMID:40069332

The antibody repertoire of autoimmune sensory neuronopathies targets pathways of the innate and adaptive immune system. An autoantigenomic approach., PMID:40026393

Reply to the letter "New perspectives in the management of SARS-CoV-2 Pirola variants and the development of long COVID syndrome: anti-Ro52/TRIM21 antibodies and qRT-PCR"., PMID:39832327

New perspectives in the management of SARS-CoV-2 Pirola variants and the development of long COVID syndrome: anti-Ro52/TRIM21 antibodies and qRT-PC., PMID:39832320

Staudinger Reaction-Responsive Coacervates for Cytosolic Antibody Delivery and TRIM21-Mediated Protein Degradation., PMID:39805770

CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression., PMID:39689859

Generation and Characterization of a TRIM21 Overexpressing Mouse Line., PMID:39487578

Evidence that autoantibody production may be driven by acute Epstein-Barr virus infection in Sjögren's disease., PMID:39472059

Antibodies targeting the Crimean-Congo Hemorrhagic Fever Virus nucleoprotein protect via TRIM21., PMID:39455551

Co-opting templated aggregation to degrade pathogenic tau assemblies and improve motor function., PMID:39276772

Aggregate-selective removal of pathological tau by clustering-activated degraders., PMID:39208111

Roles of TRIM21/Ro52 in connective tissue disease-associated interstitial lung diseases., PMID:39165359

Zn0-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro., PMID:39129512

Identification of Pathogenic Pathways for Recurrence of Focal Segmental Glomerulosclerosis after Kidney Transplantation., PMID:39125467

Nasal tau immunotherapy clears intracellular tau pathology and improves cognitive functions in aged tauopathy mice., PMID:38959324

Coexistence of systemic sclerosis and cryopyrin-associated periodic syndrome., PMID:38940218

TRIM21 and Fc-engineered antibodies: decoding its complex antibody binding mode with implications for viral neutralization., PMID:38938560

Remodeling of anti-tumor immunity with antibodies targeting a p53 mutant., PMID:38886748

Serum levels of anti-Ro52/TRIM21 antibodies in SARS-CoV-2 genetic variants and long COVID. Beyond their application in autoimmune diseases., PMID:38753568

Pin1-Catalyzed Conformation Changes Regulate Protein Ubiquitination and Degradation., PMID:38727267

Multifaceted role of TRIM21 in inflammation., PMID:38717710

Prevalence of anti-Ro52-kDa/SSA (TRIM21) antibodies and associated clinical phenotype in systemic sclerosis: Data from a French cohort, a systematic review and meta-analysis., PMID:38555075

Retrospective analysis of the clinical significance of Ro52/TRIM21 antibody and specific antinuclear antibody patterns by indirect immunofluorescence., PMID:38419219

Systems genetics of influenza A virus-infected mice identifies TRIM21 as a critical regulator of pulmonary innate immune response., PMID:38331257

Characterised intron retention profiles in muscle tissue of idiopathic inflammatory myopathy subtypes., PMID:38302260

Association of Combined Anti-Ro52/TRIM21 and Anti-Ro60/SSA Antibodies With Increased Sjögren Disease Severity Through Interferon Pathway Activation., PMID:38130019

The interconnected roles of TRIM21/Ro52 in systemic lupus erythematosus, primary Sjögren's syndrome, cancers, and cancer metabolism., PMID:37993883

Identification of Diagnosis and Typological Characteristics Associated with Ferroptosis for Ulcerative Colitis via Bioinformatics and Machine Learning., PMID:37957905

A Interacting Model: How TRIM21 Orchestrates with Proteins in Intracellular Immunity., PMID:37922533

A TRIM21-based bioPROTAC highlights the therapeutic benefit of HuR degradation., PMID:37925433

Ro52/TRIM21 - From host defense to autoimmunity., PMID:37857191

TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing., PMID:37816088

Protective mechanisms of nonneutralizing antiviral antibodies., PMID:37796829

EGFR-Induced and c-Src-Mediated CD47 Phosphorylation Inhibits TRIM21-Dependent Polyubiquitylation and Degradation of CD47 to Promote Tumor Immune Evasion., PMID:37541303

LANCL1, a cell surface protein, promotes liver tumor initiation through FAM49B-Rac1 axis to suppress oxidative stress., PMID:37540188

The Toll-like Receptor 7-Mediated Ro52 Antigen-Presenting Pathway in the Salivary Gland Epithelial Cells of Sjögren's Syndrome., PMID:37445456

Validation of an indirect ELISA assay for assessment of autoantibodies against full-length TRIM21 and its individual domains., PMID:37379227

Plasma proteomic profiling reveals KRT19 could be a potential biomarker in patients with anti-MDA5+ dermatomyositis., PMID:37160775

Serological intermolecular epitope spreading in a patient with primary Sjögren's syndrome., PMID:37130648

Trim-Away ubiquitinates and degrades lysine-less and N-terminally acetylated substrates., PMID:37061529

Isolated anti-Ro52 identifies a severe subset of Sjögren's syndrome patients., PMID:37006271

Cytosolic antibody receptor TRIM21 is required for effective tau immunotherapy in mouse models., PMID:36996217

Anti-Ro52/TRIM21 antibodies are associated with aberrant inflammatory circuits in patients with systemic autoimmune rheumatic diseases., PMID:36930561

TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away., PMID:36807144

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