Catalog No.
AHN08401
Biological activity
Measured by its binding ability in a functional ELISA. When recombinant human R‑Spondin 3 is coated at 0.25 μg/mL, recombinant human ZNRF3 (Cat #: AHN08401) binds with an apparent Kd<3 nM.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
Lys56-Met219
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9ULT6
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
KIAA1133, RNF203, RING-type E3 ubiquitin transferase ZNRF3, ZNRF3, RING finger protein 203, Zinc/RING finger protein 3, E3 ubiquitin-protein ligase ZNRF3
The impact of an RNA-binding protein group on regulating the RSPO-LGR4/5-ZNRF3/RNF43 module and the immune microenvironment in hepatocellular carcinoma., PMID:40264052
A LncRNA panel within EpCAM-specific exosomes for noninvasive early diagnosing non-small cell lung cancer., PMID:40223051
Qingre Huayu Jianpi decoction suppresses colorectal tumorigenesis by inhibiting cancer stem cells and cancer-associated fibroblasts., PMID:40220938
Structure-Guided Development of Chemically Tailored Peptide Binders of RNF43/ZNRF3 to Enable Versatile Design of Membrane Protein-Targeting PROTACs., PMID:40000409
2-Cyanopyrimidine-Containing Molecules for N-Terminal Selective Cyclization of Phage-Displayed Peptides., PMID:39772425
Structural insights into the LGR4-RSPO2-ZNRF3 complexes regulating WNT/β-catenin signaling., PMID:39753551
Stem Cell Markers LGR5, LGR4 and Their Immediate Signalling Partners are Dysregulated in Preeclampsia., PMID:39688759
Lack of dominant-negative activity for tumor-related ZNRF3 missense mutations at endogenous levels., PMID:39674817
Coactivation of Tie2 and Wnt signaling using an antibody-R-spondin fusion potentiates therapeutic angiogenesis and vessel stabilization in hindlimb ischemia., PMID:39607038
RNF43 and ZNRF3: Versatile regulators at the membrane and their role in cancer., PMID:39551397
Wnt specifically induces FZD5/8 endocytosis and degradation and the involvement of RSPO-ZNRF3/RNF43 and DVL., PMID:39463927
The molecular genetics of adrenal cushing., PMID:39388056
A new perspective on selenium's impact on renal function: European population-based analysis of plasma proteome-mediated Mendelian randomization study., PMID:39329105
Znrf3 exon 2 deletion mice do not recapitulate congenital adrenal hypoplasia., PMID:39235352
[Genomic profiles and immune microenvironment of olfactory neuroblastoma]., PMID:39231744
An "R-spondin code" for multimodal signaling ON-OFF states., PMID:39180250
Deleterious ZNRF3 germline variants cause neurodevelopmental disorders with mirror brain phenotypes via domain-specific effects on Wnt/β-catenin signaling., PMID:39168120
New Target(s) for RNF43 Regulation: Implications for Therapeutic Strategies., PMID:39125653
QPH-FR: A Novel Quinoa Peptide Enhances Chemosensitivity by Targeting Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5 in Colorectal Cancer., PMID:39047262
Clinical implications of Wnt pathway genetic alterations in men with advanced prostate cancer., PMID:39019980
E3 ligases RNF43 and ZNRF3 display differential specificity for endocytosis of Frizzled receptors., PMID:38969364
Proteomic insights into the regulatory function of ARID1A in colon cancer cells., PMID:38966585
Get your receptors in a knot with new Wnt signaling agonists., PMID:38906109
Targeted protein degradation systems to enhance Wnt signaling., PMID:38847394
Comprehensive analysis of zinc and ring finger 3 in prognostic value and pan-cancer immunity., PMID:38457275
R-Spondin 2 governs Xenopus left-right body axis formation by establishing an FGF signaling gradient., PMID:38307837
Loss of ZNRF3/RNF43 Unleashes EGFR in Cancer., PMID:38260423
Homeostasis, injury, and recovery dynamics at multiple scales in a self-organizing mouse intestinal crypt., PMID:38063302
Potent and selective binders of the E3 ubiquitin ligase ZNRF3 stimulate Wnt signaling and intestinal organoid growth., PMID:38056465
Single-Exon Deletions of ZNRF3 Exon 2 Cause Congenital Adrenal Hypoplasia., PMID:37878959
LGR4 and LGR5 form distinct homodimers that only LGR4 complexes with RNF43/ZNRF3 to provide high affinity binding of R-spondin ligands., PMID:37402772
ROTACs leverage signaling-incompetent R-spondin for targeted protein degradation., PMID:37321224
All-Trans Retinoic Acid-Responsive LGR6 Is Transiently Expressed during Myogenic Differentiation and Is Required for Myoblast Differentiation and Fusion., PMID:37240382
Receptor Elimination by E3 Ubiquitin Ligase Recruitment (REULR): A Targeted Protein Degradation Toolbox., PMID:37011906
RSPO3 Furin domain-conjugated liposomes for selective drug delivery to LGR5-high cells., PMID:36813038
Additional evidence for the role of chromosomal imbalances and SOX8, ZNRF3 and HHAT gene variants in early human testis development., PMID:36631813
Loss of Hepatic Leucine-Rich Repeat-Containing G-Protein Coupled Receptors 4 and 5 Promotes Nonalcoholic Fatty Liver Disease., PMID:36410420
Cartilage oligomeric matrix protein acts as a molecular biomarker in multiple cancer types., PMID:36255654
Antibody targeting of E3 ubiquitin ligases for receptor degradation., PMID:36131013
The role of roof plate-specific spondins in liver homeostasis and disease., PMID:39958194
Receptor control by membrane-tethered ubiquitin ligases in development and tissue homeostasis., PMID:35817504
A Comprehensive Investigation of Steroidogenic Signaling in Classical and New Experimental Cell Models of Adrenocortical Carcinoma., PMID:35563746
Roadmap for Optimizing and Broadening Antibody-Based PROTACs for Degradation of Cell Surface Proteins., PMID:35481357
Objective assessment of adrenocortical carcinoma driver genes and their correlation with tumor pyruvate kinase M2., PMID:35189247
The earliest events in BRAF-mutant colorectal cancer: exome sequencing of sessile serrated lesions with a tiny focus dysplasia or cancer reveals recurring mutations in two distinct progression pathways., PMID:35143042
Characterization of RNF43 frameshift mutations that drive Wnt ligand- and R-spondin-dependent colon cancer., PMID:35040131
RNF43/ZNRF3 loss predisposes to hepatocellular-carcinoma by impairing liver regeneration and altering the liver lipid metabolic ground-state., PMID:35039505
Circ_0000190 sponges miR-382-5p to suppress cell proliferation and motility and promote cell death by targeting ZNRF3 in gastric cancer., PMID:35037032
The RSPO-LGR4/5-ZNRF3/RNF43 module in liver homeostasis, regeneration, and disease., PMID:35006616
RNF43/ZNRF3 negatively regulates taste tissue homeostasis and positively regulates dorsal lingual epithelial tissue homeostasis., PMID:34995498
For research use only. Not for human or drug use.
