Catalog No.
ARD83901
Biological activity
Measured in a cell proliferation assay using PHA activated mouse splenocytes. The ED50 for this effect is 0.025-0.25 ng/mL.
Expression system
Mammalian Cells
Species
Rattus norvegicus (Rat)
Protein length
IL12A [Arg23-Ser215] & IL12B [Met23-Ser335]
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9R278 & Q9R103
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
IL-12A, NK cell stimulatory factor chain 1, IL12A, CLMF p35, Interleukin-12 subunit alpha, IL-12 subunit p35, NKSF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit
Inflammatory and Angiogenic Mediators Are Differentially Ex-Pressed in Patients with Post-COVID-19 Syndrome with Normal and Abnormal Spirometry Results., PMID:40508959
Giardia increases macrophage production of the anti-inflammatory cytokine interleukin-10 in response to lipopolysaccharide via macrophage galactose binding lectin., PMID:40493880
Combining local cytokine delivery and systemic immunization with recombinant artLCMV boosts antitumor efficacy in several preclinical tumor models., PMID:40492380
Flagellins as Vaccine Adjuvants and Cancer Immunotherapy: Recent Advances and Future Prospects., PMID:40491306
Preclinical and clinical evaluation of intratumoral injection of an IL-12 expressing SKV-012 oncolytic virus for advanced solid tumors., PMID:40484644
Recombinant BCG Expressing IL-12 as A Novel Immunomodulatory Strategy for Allergic Asthma: Opportunities and Challenges., PMID:40483388
Membrane-IL12 adjuvant mRNA vaccine polarizes pre-effector T cells for optimized tumor control., PMID:40479650
An Il12 mRNA-LNP adjuvant enhances mRNA vaccine-induced CD8 T cell responses., PMID:40478935
Bioinformatics analysis of innovative multi-epitope vaccine utilizing MAGE-A, MAM-A, and Gal-3 for breast cancer management., PMID:40473741
Clinical characteristics and genetic features of 35 cases of adverse reactions to Bacillus Calmette-Guérin vaccine in children., PMID:40470261
OVATION-2: A randomized phase I/II study evaluating the safety and efficacy of IMNN-001 (IL-12 gene therapy) with neo/adjuvant chemotherapy in patients newly-diagnosed with advanced epithelial ovarian cancer., PMID:40461366
[Network pharmacology and molecular docking explore mechanism of Croci Stigma in treating immune checkpoint inhibitor-associated myocarditis]., PMID:40461207
EXPRESS: Key inflammatory mediators drive the resolution of acute Lyme disease., PMID:40459009
Hepatic ferroptosis induced by Clonorchis sinensis exacerbates liver fibrosis., PMID:40455823
Sacha inchi (Plukenetia volubilis) seed oil alleviates Plasmodium berghei infection in mice by reducing proinflammatory cytokine levels and inhibiting parasite growth., PMID:40453844
miRNA let-7-5p present in the extracellular vesicles of Trichinella spiralis newborn larvae inhibits the function of M1-type RAW264.7 macrophages by targeting C/EBPδ., PMID:40452071
A cross-sectional study investigating the underlying inflammatory mechanisms of post-stroke insomnia., PMID:40450992
A virus based vaccine combined with IL12 gene therapy eradicates aggressive melanoma., PMID:40442309
The role of IL-39 in autoimmune diseases: From general to immunopathogenesis., PMID:40440970
JAK-STAT inhibitors in noninfectious uveitis - A review., PMID:40434456
[Effects of Toxoplasma gondii type I/II rhoptry protein 16 on the polarization and inflammatory response of mouse alveolar macrophages]., PMID:40425495
Association of Interleukin-12A Genotypes With Nasopharyngeal Carcinoma Risk., PMID:40425357
Recombinant C5a Peptidase and Formalin-Killed Cell: A Synergistic Vaccine Against Streptococcus iniae in Four-Finger Threadfin Fish (Eleutheronema tetradactylum)., PMID:40420522
A cohort study on the association between metabolic/inflammatory status and pregnancy complications in PCOS patients after IVF/ICSI treatment., PMID:40419888
Immune, metabolic, anatomical, and functional features of people after successful tuberculosis treatment: an exploratory analysis., PMID:40419526
Esculetin inhibited fever, pain, and inflammatory responses via binding to HSC70., PMID:40412774
Toxoplasma gondii C2 Domain Protein Deletion Mutant as a Promising Vaccine Against Toxoplasmosis in Mice., PMID:40407413
Solid tumor CAR T cells engineered with fusion proteins targeting PDL1 for localized IL-12 delivery., PMID:40406466
The relationship between ruminal mat consistency of calves and mortality or immunity., PMID:40404739
Harnessing local and system immune profiling delineating differential responders to first-line sintilimab (anti-PD-1 antibody) combined with chemotherapy in extensive-stage small cell lung cancer: an exploratory biomarker analysis of a phase II study., PMID:40404633
FcRn-silencing of IL-12Fc prevents toxicity of local IL-12 therapy and prolongs survival in experimental glioblastoma., PMID:40404625
Onboard, tethered IL-12 boosts potency of the Tmod NOT gate and preserves selectivity., PMID:40404208
Boosting immune response against cervical cancer: A combined approach using oncolytic virus and targeted therapies., PMID:40403026
The 'totality of evidence' and 'extrapolation' of SB17, a ustekinumab biosimilar., PMID:40396611
The evaluation of a novel multi-epitope vaccine against human papillomavirus and Candida albicans., PMID:40390500
Immunization of BALB/c mice with detoxified lipopolysaccharide and hydrolytic O-polysaccharide from Brucella melitensis either in combination with or conjugated to tetanus toxoid, enhances protective immune responses against the pathogen., PMID:40383101
Hepatic encephalopathy induces anxiety and depression-like behaviors, cytokine dysfunction, BDNF down-regulation and neuropathological changes in mice., PMID:40381891
Chrysin-loaded PLGA nanoparticle attenuates ferroptosis in lipopolysaccharide-induced indirect acute lung injury by upregulating Nrf2-dependent antioxidant responses., PMID:40379234
A comparative study on immune responses to demineralized and decellularized bone substitute following intraperitoneal implantation in mouse model., PMID:40378388
Antioxidant interventions reduced cytokine-induced pyroptosis of peripheral MAIT cells in patients with HBV-related cirrhosis., PMID:40377492
Immune-microbiota dysregulation in maintenance hemodialysis: a 16S rRNA sequencing-based analysis of gut flora and T cell profiles., PMID:40375064
Predicting ustekinumab treatment response in Crohn's disease using pre-treatment biopsy images., PMID:40366737
Chrysin-loaded poly (lactic-co-glycolic acid) nanoparticles alleviate sepsis-induced splenic injury by regulating myeloid-derived suppressor cells., PMID:40358797
Anti-IL-12/23p40 antibodies for induction of remission in Crohn's disease., PMID:40357993
rFIP-nha activates macrophages towards a pro-inflammatory phenotype via AIM2 inflammasome modulation., PMID:40356602
Relation of Cytokine Profile and Antibody Values to Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis (Preliminary Data)., PMID:40353964
[Experimental study on Jianpi Qutan Formula regulating M1/M2 macrophage polarization to improve atherosclerosis]., PMID:40350948
Helja lectin inhibits Candida albicans phagocytosis and induces pro-inflammatory responses in dendritic cells., PMID:40347922
Immunotherapeutic efficacy of Th1 stimulatory epitopes derived from Leishmania donovani enolase and triose phosphatase isomerase in experimental visceral leishmaniasis., PMID:40347781
A sulfated fucopyranosyl-glucosamino-glucopyranose from marine heterotrophic Bacillus tequilensis regulates inflammatory cytokines in lipopolysaccharide-stimulated human monocyte THP-1 cells., PMID:40345301
For research use only. Not for human or drug use.
