Catalog No.
AHH37701
Biological activity
Measured by its ability to cleave a fluorogenic peptide substrate, McaYVADAPK(Dnp)-OH. The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
Val22-Gln601
Nature
Recombinant
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Q16819
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Endopeptidase-2, PABA peptide hydrolase, Meprin A subunit alpha, MEP1A, PPH alpha, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
Note
For research use only.
A HhaI polymorphism in the human MEP1A gene encoding the alpha subunit of the metalloendopeptidase meprin., PMID:8854145
A tissue specific approach for analysis of membrane and secreted protein antigens from Haemonchus contortus gut and its application to diverse nematode species., PMID:9879887
Identification and localization of MEP1A-like sequences (MEP1AL1-4) in the human genome., PMID:10405340
Exclusion of the juvenile myoclonic epilepsy gene EFHC1 as the cause of migraine on chromosome 6, but association to two rare polymorphisms in MEP1A and RHAG., PMID:16378686
Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp)., PMID:20687161
Balance of meprin A and B in mice affects the progression of experimental inflammatory bowel disease., PMID:21071511
TNF-α-induced down-regulation of CDX2 suppresses MEP1A expression in colitis., PMID:22326557
Post-transcriptional regulation of meprin α by the RNA-binding proteins Hu antigen R (HuR) and tristetraprolin (TTP)., PMID:23269677
Metalloproteases meprin α and meprin β are C- and N-procollagen proteinases important for collagen assembly and tensile strength., PMID:23940311
The role of CDX2 in inflammatory bowel disease., PMID:24814920
A genetic screen in Drosophila for regulators of human prostate cancer progression., PMID:25117438
endoProteoFASP: a novel FASP approach to profile salivary peptidome and disclose salivary proteases., PMID:25476335
Hyperoxia-Induced Protein Alterations in Renal Rat Tissue: A Quantitative Proteomic Approach to Identify Hyperoxia-Induced Effects in Cellular Signaling Pathways., PMID:26106253
Origin and Diversification of Meprin Proteases., PMID:26288188
Metalloproteases meprin-ɑ (MEP1A) is a prognostic biomarker and promotes proliferation and invasion of colorectal cancer., PMID:27378469
Deficiency of the DSPP-cleaving enzymes meprin α and meprin β does not result in dentin malformation in mice., PMID:27628095
Transient and Prolonged Response of Chicken Cecum Mucosa to Colonization with Different Gut Microbiota., PMID:27685470
Metalloproteinase meprin α regulates migration and invasion of human hepatocarcinoma cells and is a mediator of the oncoprotein Reptin., PMID:27999200
Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Mice by Regulating Meprin α., PMID:31877411
Meprin-α (Mep1A) enhances TNF-α secretion by mast cells and aggravates abdominal aortic aneurysms., PMID:32072633
Investigating the human protein-host protein interactome of SARS-CoV-2 infection in the small intestine., PMID:33244381
Mep1a contributes to Ang II-induced cardiac remodeling by promoting cardiac hypertrophy, fibrosis and inflammation., PMID:33301800
Pathophysiological Implications of Urinary Peptides in Hepatocellular Carcinoma., PMID:34359689
Ivory poaching and the rapid evolution of tusklessness in African elephants., PMID:34672738
Differential urine proteome analysis of a ventilator-induced lung injury rat model by label-free quantitative and parallel reaction monitoring proteomics., PMID:34728735
Identification of Mep1a as a susceptibility gene for atherosclerosis in mice., PMID:34849841
Neurobiochemical, Peptidomic, and Bioinformatic Approaches to Characterize Tauopathy Peptidome Biomarker Candidates in Experimental Mouse Model of Traumatic Brain Injury., PMID:36635478
A novel predictive model based on inflammatory response-related genes for predicting endometrial cancer prognosis and its experimental validation., PMID:37276865
Identification and validation of key genes associated with pathogenesis and prognosis of gastric cancer., PMID:37868053
The effect of rs2910686 on ERAP2 expression in IBD and epithelial inflammatory response., PMID:39123229
Probing of MEP1A gene to identify biomarkers associated with post-partum anestrus in buffalo., PMID:40105636
Oral exposure to nanoplastics and food allergy in mice fed a normal or high-fat diet., PMID:40252413
For research use only. Not for human or drug use.
