Catalog No.
AMD85401
Biological activity
Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL4. The ED50 for this effect is 0.07-0.35 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
Expression system
Mammalian Cells
Species
Mus musculus (Mouse)
Protein length
Gly115-Leu260
Nature
Recombinant
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
P27548
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, sCD40L, Cd40lg, Cd40l, Tnfsf5
Note
For research use only.
Deficiency of adenosine deaminase 2 skews adaptive immune repertoires toward specific sets of T- and B-cell receptors., PMID:39924119
Discrimination of Anti-Donor Response in Allogeneic Transplantation Using an Alloreactive T-Cell Detection Assay., PMID:39936124
CD40 induces PIR-A+ macrophages to promote chronic allograft rejection., PMID:39954660
Pro-inflammatory and anti-inflammatory immune biomarkers as predictors of neurodevelopment in young children exposed to HIV., PMID:39970188
Interleukin-10 production by innate lymphoid cells restricts intestinal inflammation in mice., PMID:39988202
Novel plasma biomarkers of amyloid plaque pathology and cortical thickness: Evaluation of the NULISA targeted proteomic platform in an ethnically diverse cohort., PMID:39989429
Modified CD40L-Activated B-Cell Proliferation Model for Validating the Suppressive Activity of CD40-CD154 Pathway Inhibitors., PMID:39994946
Administration of a Recombinant Fusion Protein of IFN-γ and CD154 Inhibited the Infection of Chicks with Salmonella enterica., PMID:40005871
Clustering Mycobacterium tuberculosis-specific CD154+CD4+ T cells for distinguishing tuberculosis disease from infection based on single-cell RNA-seq analysis., PMID:40010539
Regulatory effect of curcumin on CD40:CD40L interaction and therapeutic implications., PMID:40043936
Foot-and-mouth disease virus-like particle vaccine incorporating dominant T and B cell epitopes: enhanced immune response in piglets with CD154 molecules., PMID:40046411
Transition from preclinical to clinical application of CTLA4-Ig co-stimulation blockage in beta-cell replacement therapy., PMID:40048867
Influence of environmental exposures on T follicular helper cell function and implications on immunity: a comparison of Bangladeshi and American children., PMID:40062864
Fc-mediated immune stimulating, pro-inflammatory and antitumor effects of anti-HER2 IgE against HER2-expressing and trastuzumab-resistant tumors., PMID:40074330
Efficacy of ticagrelor and clopidogrel in treating unstable angina and their effects on serum inflammatory factors., PMID:40089933
Blunted CD40-responsive enhancer activation in CREBBP-mutant lymphomas can be restored by enforced CD4 T-cell engagement., PMID:40090010
Lupenone preserves T cell activity by recovery of CD40L expression and protection from cytotoxicity due to methamphetamine exposure., PMID:40111983
Diagnostic value of sTREM-1, sCD25, sCD40L and sCD130 in late-onset neonatal sepsis., PMID:40118381
Engineered vesicular cancer vaccines for immunosuppressive microenvironment reversion and in situ vaccine generation., PMID:40122241
Salvianolic Acid A From Salvia miltiorrhiza Suppresses Endometrial Carcinoma Progression via CD40-AKT-NF-κB Pathway., PMID:40134253
Extensive T-Cell Profiling Following SARS-CoV-2 mRNA Vaccination in Multiple Sclerosis Patients Treated with DMTs., PMID:40137720
Effect of edaravone dexborneol combined with interventional thrombectomy on nerve function, cerebral hemodynamics, and serum levels of serum amyloid A, lipoprotein-associated phospholipase A2, and soluble CD40 ligand in elderly patients with ischemic stroke., PMID:40137846
Reduced Serum PD-L1 and Markers of Inflammation in Response to Alternate Day Fasting With a Low-Carbohydrate Intervention: A Secondary Analysis of a Single-Arm Trial., PMID:40145019
Targeted immunotherapies for Graves' thyroidal & orbital diseases., PMID:40145088
Temporal immune profiling in the cerebrospinal fluid and blood of patients with aneurysmal subarachnoid hemorrhage., PMID:40154495
sCD40 and sCD40L as candidate biomarkers of rheumatic diseases: a systematic review and meta-analysis with meta-regression., PMID:40176806
Enhancing antitumor immunity through chemotherapeutic-derived lipid nanoparticle-induced immunogenic cell death and CD40L/Flt3L mRNA-mediated dendritic cell activation., PMID:40185331
CAR Treg synergy with anti-CD154 promotes infectious tolerance and dictates allogeneic heart transplant acceptance., PMID:40197364
CD40L stimulates tumor-infiltrating B-cells and improves ex vivo TIL expansion., PMID:40199608
Mathematical modeling unveils the timeline of CAR-T cell therapy and macrophage-mediated cytokine release syndrome., PMID:40203243
Recognition of different subsets of alloreactive T cells by activation-induced markers., PMID:40204006
Construction and evaluation of a 180-day readmission prediction model for chronic heart failure patients based on sCD40L., PMID:40228270
OMIP-113: Characterization of Cytokine Producing T Cells in Swine., PMID:40231482
CD4+ T-cell help delivery to monocyte-derived dendritic cells promotes effector differentiation of helper and cytotoxic T cells., PMID:40239818
The T-cell response to SARS-CoV- 2 vaccination persists beyond six months in rheumatoid arthritis patients treated with rituximab., PMID:40241223
Ex Vivo Overactivation of Lymphocyte Subsets in Fibrotic Hypersensitivity Pneumonitis Is Blunted by a Sphingosine-1-Phosphate Receptor Ligand., PMID:40243992
LILRB2 blockade facilitates macrophage repolarization and enhances T cell-mediated antitumor immunity., PMID:40246582
Genetic evidence from Mendelian randomization links CD40 levels to increased risk of estrogen receptor-positive breast cancer., PMID:40295650
A novel CD40LG mutation causing X‑linked hyper-IgM syndrome., PMID:40330326
Long Noncoding RNA H19 Regulates the Foam Cell Formation of Vascular Smooth Muscle Cells by Inhibiting microRNA-107 to Activate the CD40/CD40L Pathway., PMID:40340885
Case Report: Co-occurrence of tubulitis and SARS-CoV-2 specific T-cells in a kidney transplant recipient., PMID:40343198
Platelet-Derived Soluble CD40L and Its Impact on Immune Modulation and Anti-IL6R Antibody Treatment Outcome in Rheumatoid Arthritis., PMID:40358149
Evaluation of Plasma Soluble Cd40 Ligand Levels in Children with Familial Mediterranean Fever and Its Relationship with Carotid Intima-Media Thickness., PMID:40361895
Platelets and MMP‑9 contribute to esophageal cancer invasion via CD40‑CD154 interactions., PMID:40376974
Case Report: CD40LG Arg203Ile variant underlies atypical phenotype of X-linked hyper IgM syndrome., PMID:40391217
CD8+ T cell-derived CD40L mediates noncanonical cytotoxicity in CD40-expressing cancer cells., PMID:40397730
Exploring the Causal Association Between 91 Circulating Inflammatory Proteins and Neurodegenerative Diseases: A Bidirectional Two-Sample Mendelian Randomization and Bioinformatics Analysis., PMID:40462656
In-depth characterization of vaccine-induced neoantigen-specific T cells in patients with IDH1-mutant glioma undergoing personalized peptide vaccination., PMID:40480654
CD154:CD11b blockade enhances CD8+ T cell differentiation during infection but not transplantation., PMID:40485581
Activated CD4+ T cells upregulate PD-L1 expression on B cells through CD40/CD40L signaling and direct contact in systemic lupus erythematosus., PMID:40499670
For research use only. Not for human or drug use.
