Recombinant Mouse C3/Complement C3 Protein, C-His (Active)

Catalog No.

AMB89901

Biological activity

Measured by its ability to enhance IL-6 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is typically 0.8-4 µg/mL.

Expression system

E. coli

Species

Mus musculus (Mouse)

Protein length

Ser671-Arg748

Nature

Recombinant

Applications

Bioactivity, ELISA, Immunogen, SDS-PAGE, WB

Endotoxin level

<1 EU/μg of the protein by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Accession

P01027

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Complement C3, HSE-MSF, Complement C3 beta chain, C3-beta-c, C3bc, Complement C3 alpha chain, C3a anaphylatoxin, Acylation stimulating protein, ASP, C3adesArg, Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3dg fragment, Complement C3g fragment, Complement C3d fragment, Complement C3f fragment, Complement C3c alpha' chain fragment 2, C3

Note

For research use only.

SDS-PAGE

SDS-PAGE for Recombinant Mouse C3 protein
Bioactivity

(D) Binding of purified anti-FXIII autoantibody to complement C3, C4, or C5.
Data sourced from citation (PMID: 41364521)
Bioactivity

Figure 3. Autoimmune FXIII‑C3‑deficient mice had severe pathogenic infections with C. albicans. (A) Coomassie staining of fusion protein C3‑FXIII. The left side of the gel contains different amounts of C3‑FXIII fusion protein (0.25, 0.5, and 1 μg), the right side contains different amounts of the control protein BSA (0.5, 1, and 2 μg). (B) Tail bleeding time of different groups of mice. (C) Survival rate of different groups of mice. A dose of 1 × 10⁵ C. albicans per mouse was applied (n = 10 per group). The infection period was 15 days. (D) H&E staining for kidney pathology upon fungal infection. Representative images out of 6 are shown. (E) Renal injury score evaluated by images of H&E staining. (F) Fungal burdens of different organs. For these in vivo experiments, C57BL/6J mice were first immunized by PBS, FXIII, C3, or C3‑FXIII, generating different autoimmune‑deficient mice, simply named WT, FXIII, C3, and FXIII‑C3. These mice were further challenged with C. albicans, generating 8 groups of mice, which were classified as WT/Ca and WT/PBS, FXIII/Ca and FXIII/PBS, C3/Ca and C3/PBS, FXIII‑C3/Ca and FXIII‑C3/PBS. For (B) and (D–F), 5 × 10⁴ C. albicans per mouse was applied (n = 6 per group). Infection time is 7 days. Data are shown as mean ± SD; one‑way ANOVA was used for statistical analysis. The survival rate of mice was analyzed by a Kaplan‑Meier log‑rank test. Scale bar: 100 μm. **P < 0.01, ***P < 0.001.
Data sourced from citation (PMID: 41364521)
Bioactivity

Figure 5. Autoimmune FXIII‑C3‑deficient mice had defective complement and T cell activation. (A) C3b/iC3b deposition and T cell infiltration in kidney were analyzed by immunofluorescent staining. Representative images (out of 6) are shown. (B and C) CD4 and CD8 T cell activation upon fungal infection. T cell activation was quantified by the CD44highCD62Llow portion of CD4+ and CD8+ T cells via flow cytometry. (D and E) Portion of IFN‑γ+ or IL‑17+ CD4 and CD8 T cells analyzed by flow cytometry. In the in vivo infection model, WT, FXIII, C3, and FXIII‑C3 groups of mice were intravenously injected with C. albicans (5 × 10⁴/mouse) or PBS for 7 days; 8 groups of mice were classified as WT/Ca and WT/PBS, FXIII/Ca and FXIII/PBS, C3/Ca and C3/PBS, or FXIII‑C3/Ca and FXIII‑C3/PBS (n = 6 per group). Data are shown as mean ± SD, one‑way ANOVA was used for statistical analysis. Scale bar: 40 μm, original magnification, ×400. **P < 0.01, ***P < 0.001
Data sourced from citation (PMID: 41364521)
Bioactivity

Figure 6. Pathogenic C. albicans infections further induced autoantibody generation in mice. Combined FXIII-C3 autoantibody (A), and anti-FXIII auto-antibody (B) in 8 groups of mice on days 1, 5, and 7 after infection were detected by ELISA. (C) FXIII-A antigen level in 8 groups of mice on days 1, 5, and 7 after infection. The FXIII-A antigen level of WT/PBS group on day 1 was set as 100%, the other samples were calculated accordingly. (D) Total B cell activation. Cells were isolated from the spleen and analyzed by flow cytometry. B cell activation is shown by the percentage of CD19+ CD69+ B cells. (E) Autoreactive B cell activation. Again, cells were isolated from the spleen and analyzed by flow cytometry. Anti-FXIII IgG+ autoreactive B cells activation is presented by the percentage of anti-FXIII IgG+ B subgroup among CD19+ CD69+ B cells. In this in vivo experimental settings, WT, FXIII, C3, and FXIII-C3 groups of mice were intravenously injected with C. albicans (5 x 10 4/mouse) or PBS, and 8 groups of mice were classified as WT/Ca and WT/PBS, FXIII/Ca and FXIII/PBS, C3/Ca and C3/PBS, or FXIII-C3/Ca and FXIII-C3/PBS (n = 6 per group). Data are shown as mean ± SD. An unpaired 2-tailed Student's t test was used for statistical analysis for A and B. One-way ANOVA was used for statistical analysis for C-E. *P < 0.05, **P < 0.01, ***P < 0.001
Data sourced from citation (PMID: 41364521)
Bioactivity

Figure 7. Pathogenic C. albicans infection triggered platelet activation and CD40L release. (A and B) Quantification of platelets' activation in 8 different groups of mice via CD62p surface expression analyzed by flow cytometry. A representative dot plot (A) and statistical chart of activated platelets (B) are shown. (C) Platelet activation upon in vitro co-culture of platelets with C. albicans. CD62p expression (left) and GPIIbIIIa expression (right) on platelet surfaces were analyzed by flow cytometry upon in vitro co-culture of platelets (1 x 107 /sample) with different doses of C. albicans (1 x 105 , 1 x 106 and 1 x10⁷ /sample) in the presence of Gly-Pro-Arg-Pro (Pefa 6003) to prevent platelet aggregation. (D) Soluble CD40L levels in plasma of 8 groups of mice on day 7 after infection. (E) Soluble CD40L levels in plasma of patients with FXIII deficiency and in healthy control donors. (F) CD40L release in the platelet-C. albicans co-culture supernatant. Data are shown as mean ± SD; 1-way ANOVA was used for statistical analysis. **P < 0.01, ***P < 0.001. FSC-A, forward scatter area; PLA, proximity ligation assay; APC-A, allophycocyanin area.
Data sourced from citation (PMID: 41364521)

Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency. [AMB89901]

De novo assembly of multidrug resistant biofilm forming Micrococcus luteus genome from hemodialysis tunneled cuffed catheter tips of patients undergoing renal failure treatment., PMID:40444959

Streptococcus suis AdcA interacts with factor H and inhibits C3b deposition on the bacteria to participate in complement evasion., PMID:40446560

Molecular characterization and environmental response of dissolved organic matter in reserve quiescent groundwater wells of the North China plain: Insights from spectroscopy and mass spectrometry., PMID:40449357

Graphitic carbon nitride/methyl pyrophaeophorbide a-copper/folate nanoconjugate for enhanced immunogenic death combined with PD-L1 immune checkpoint blockades., PMID:40450918

Self-assembly of Urea Derivatives into Supramolecular Gels., PMID:40451817

Pterosin D-activated protein kinase A mitigates Alzheimer's disease in 5xFAD mice., PMID:40452366

Factor H-related 1 and heparan sulfate architecture contribute to complement dysregulation in C3 glomerulopathy., PMID:40453077

Evidence of Heteroepitaxy and Solid Solutions in Lattice Matched Ternary Covalent Organic Framework Systems., PMID:40456694

Integrative transcriptomic and proteomic analyses reveal that carbon metabolism and complement system of Madin Darby Bovine Kidney cells are affected by bovine coronavirus infection., PMID:40457360

Discovery of a Novel and Potent MET Transcription Inhibitor Targeting Promoter G-Quadruplex for the Treatment of NSCLC., PMID:40457545

Association of The Energy-Adjusted Dietary Inflammatory Index and Sjögren's Syndrome: A Cross-Sectional Study., PMID:40458010

Contrasting mediation actions on interactions of phosphorus with lanthanum modified zeolite by terrestrial- and algal-derived dissolved organic matter., PMID:40460526

Caloric restriction mimetic 2-deoxyglucose alters metabolic and transcriptomic phenotype in association with changes in chromatin accessibility in human astrocytes., PMID:40461556

Understanding freezing-induced changes in the protein and fat phases of aged Gouda cheese using Fourier transform infrared and Raman spectroscopy., PMID:40467239

Analysis of human factor H-related gene and protein expressed in rheumatoid arthritis synovium identifies a novel mechanism promoting dysregulated complement pathway activation., PMID:40467630

Complement in anti-glomerular basement membrane glomerulonephritis., PMID:40469278

Complementing muscle regeneration-fibro-adipogenic progenitor and macrophage-mediated repair of elderly human skeletal muscle., PMID:40473693

Chemoselective dual functionalization of proteins via 1,6-addition of thiols to trifunctional N-alkylpyridinium., PMID:40480996

Native globular ferritin nanopore sensor., PMID:40481000

High-fat diet induces pre-eclampsia through dampening cell-autonomous C3 in trophoblasts., PMID:40481230

Treatment with orforglipron, an oral glucagon like peptide-1 receptor agonist, is associated with improvements of CV risk biomarkers in participants with type 2 diabetes or obesity without diabetes., PMID:40481478

Adipocytes promote cancer stemness properties in oral squamous cell carcinoma through C3/C3AR axis and sphingolipid metabolism., PMID:40482908

Clinical and immunopathological assessment of the oral mucosa in coeliac disease: a pilot study., PMID:40483462

[Diagnosis, treatment, and genetic analysis of five cases of primary atypical hemolytic uremic syndrome]., PMID:40484823

Fibroblast activation protein-α is a potential prognostic biomarker related to ferroptosis in head and neck squamous cell carcinoma., PMID:40486494

Elevated eosinophil-to-lymphocyte ratio (ELR) as a predictor of relapse for IgG4-related disease: a retrospective study across a decade., PMID:40488799

Aldo-keto reductase family 1 member C3 (AKR1C3) gene polymorphism (rs12529) is associated with breast cancer in Bangladeshi population: A case-control study and computational investigation., PMID:40489436

Correlation study between clinical phenotypes and autoantibodies in systemic sclerosis in Southwest China., PMID:40489831

Low Carbohydrate Diet Containing Soy Protein and Fish Oil Reduces AOM/DSS-Induced Colon Cancer, in Part, by an Acetate-Mediated Reduction in TH17 Cell Differentiation., PMID:40491066

Biological degradation of graphitic carbon nitride sheets and autophagy induction in macrophages., PMID:40491410

Primary Sjögren's syndrome presenting with non-diffuse membranoproliferative glomerulonephritis-like lesions in cryoglobulinemic glomerulonephritis: a case report., PMID:40491909

Unlocking the therapeutic potential of protocatechualdehyde: pharmacological applications and mechanism insights., PMID:40493248

Geminiviral replication enhancer hijacks a negative regulator of cytokinin signaling to facilitate viral infection., PMID:40495381

Lecanemab Binds to Transgenic Mouse Model-Derived Amyloid-β Fibril Structures Resembling Alzheimer's Disease Type I, Type II and Arctic Folds., PMID:40495448

Perinatal death in pig models of hypertrophic cardiomyopathy carrying sarcomere pathogenic variants., PMID:40496056

BdNRT2A and BdNRT3.2 Are the Major Components of the High-Affinity Nitrate Transport System in Brachypodium distachyon., PMID:40497030

Unraveling the molecular basis of antibiotic resistance in S. aureus focusing on plasmid-mediated mechanisms., PMID:40501247

Scaled multidimensional assays of variant effect identify sequence-function relationships in hypertrophic cardiomyopathy., PMID:40501845

Effects of C3aR activated mast cells in eosinophilic esophagitis., PMID:40502038

Huangqi decoction ameliorated intestinal barrier dysfunction via regulating NF-κB signaling pathway in slow transit constipation model mice., PMID:40502512

Superior Diagnostic Efficacy of N-Terminal Propeptide of Type III Collagen and Golgi Protein 73 for Detection of Fibrosis in Chronic Hepatitis B Patients., PMID:40502814

Discriminating Disease Flare From Infection in Febrile Patients With Systemic Lupus Erythematosus in a Safety-Net Hospital System: A Multicenter Study., PMID:40503665

An Unusual Presentation of Bullous Skin Lesions During Pregnancy., PMID:40503690

Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus., PMID:40506261

Immunotherapy Platform That Conjugates Antigen to Complement C3-Targeted Liposomes Induces a Robust Adaptive Immune Response., PMID:40507796

Successful Management of C3 Glomerulopathy Recurrence Post-Kidney Transplantation with Iptacopan: A Case Report., PMID:40507864

Withania somnifera and Chrysanthemum zawadskii Herbich var. latilobum (Maxim.) Kitamura Complex Attenuates Obesity in High-Fat-Diet-Induced Obese Mice., PMID:40508039

Cucurbitane Glycosides and Their Potential Anti-Inflammatory Activities from Hemsleya chinensis Tubers., PMID:40509239

Corydalis yanhusuo Polysaccharides regulates HPA-axis mediated microglia activation and inhibits astrocyte A1 transformation to improve depression-like behavior., PMID:40513699

Synaptic pruning genes networks in Alzheimer's disease: correlations with neuropathology and cognitive decline., PMID:40515808

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