Catalog No.
AMG82801
Biological activity
Measured by its ability to cause collapse of chick embryonic dorsalroot ganglia (DRG) neuron growth cones. 20-60 ng/mL of recombinant mouse SEMA3A (Cat #: AMG82801) causes >50% growth cone collapse in the presence 10 ng/mL recombinant human beta‑NGF.
Expression system
Mammalian Cells
Species
Mus musculus (Mouse)
Protein length
Asn21-Lys747 (Arg552Ala, Arg554Ala, Arg555Ala, Arg618Ala, Lys619Ala, Lys642Ala, Lys643Ala)
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Semaphorin III, Sema III, SEMA3A, Semaphorin-3A, SEMAD
For research use only. Not for human or drug use.
