Catalog No.
AHA18501
Biological activity
Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >30,000 pmol/min/μg, measured under the described conditions.
Expression system
Mammalian Cells
Species
Homo sapiens (Human)
Protein length
Gln27-Leu559
Nature
Recombinant
Endotoxin level
<0.1 EU/μg of the protein by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Accession
O00748
Applications
Bioactivity, ELISA, Immunogen, SDS-PAGE, WB
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Cocaine esterase, CES2, Carboxylesterase 2, CE-2, ICE, Methylumbelliferyl-acetate deacetylase 2, hCE-2
Differential expression of capecitabine-related metabolic enzymes in hepatocellular carcinoma and its clinical value: a retrospective cohort study., PMID:40504344
Discovery of a Novel Serine-Targeting Covalent Inhibitor against HCES2A for Treating Drug-induced Diarrhea and Ulcerative Colitis., PMID:40396776
Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy., PMID:40359857
Discovery of cardiovascular drugs as effective inhibitors of human carboxylesterase., PMID:40339856
Exploring human carboxylesterases 1 and 2 selectivity of two families of substrates at an atomistic level., PMID:40209868
Carboxylesterase 2A gene knockout or enzyme inhibition alleviates steatohepatitis in rats by regulating PPARγ and endoplasmic reticulum stress., PMID:40089078
AI-Driven Discovery of Highly Specific and Efficacious hCES2A Inhibitors for Ameliorating Irinotecan-Triggered Gut Toxicity., PMID:40073334
Discovery of tri(indolyl)methanes as potent and selective inhibitors against human carboxylesterase 2., PMID:40068745
Functional Analysis of Modified Caco-2 Cells Carrying CES2A1 as a Model for Intestinal Absorption of Prodrugs., PMID:40010717
Protein abundance of drug transporters and drug-metabolizing enzymes in paired healthy and tumor tissue from colorectal cancer patients., PMID:39809348
Near-infrared fluorescence imaging tool with large Stokes shift for sensitively detecting carboxylesterase 2 and monitoring its expression in non-alcoholic fatty liver disease., PMID:39689640
Intestinal human carboxylesterase 2 (CES2) expression rescues drug metabolism and most metabolic syndrome phenotypes in global Ces2 cluster knockout mice., PMID:39496863
Generation and application of CES1-knockout Tet-Off-regulated CYP3A4 and UGT1A1-expressing Caco-2 cells., PMID:39383894
Sex differences in the metabolic activation of and platelet response to vicagrel in mice: Androgen as a key player., PMID:39366431
Discovery of the FXR/CES2 dual modulator LE-77 for the treatment of irinotecan-induced delayed diarrhea., PMID:39362081
A novel carboxylesterase 2-targeted fluorescent probe with cholic acid as a recognition group for early diagnosis of drug- and environment-related liver diseases., PMID:39342850
WTAP promotes the progression of ulcerative colitis by silencing the expression of CES2 through m6A modification., PMID:39141984
The metabolic activation of and platelet response to vicagrel vary with P-glycoprotein deficiency, rather than P-glycoprotein inhibition, in mice., PMID:39126503
Characterisation of ciclesonide metabolism in human placentae across gestation., PMID:38875771
Effects of alcohol consumption and tobacco smoking on the composition of the ensemble of drug metabolizing enzymes and transporters in human liver., PMID:38798409
Simultaneous determination of unecritinib (TQ-B3101) and its active metabolite crizotinib in rat plasma by LC-MS/MS：An application to pharmacokinetic studies., PMID:38744200
Regulation of carboxylesterases and its impact on pharmacokinetics and pharmacodynamics: an up-to-date review., PMID:38706437
Evaluation of the inhibitory effects of antigout drugs on human carboxylesterases in vitro., PMID:38670244
The conjugates of 5'-deoxy-5-fluorocytidine and hydroxycinnamic acids - synthesis, anti-pancreatic cancer activity and molecular docking studies., PMID:38655481
Differential Tissue Abundance of Membrane-Bound Drug Metabolizing Enzymes and Transporter Proteins by Global Proteomics., PMID:38641346
SPINK1 Overexpression Correlates with Hepatocellular Carcinoma Treatment Resistance Revealed by Single Cell RNA-Sequencing and Spatial Transcriptomics., PMID:38540686
Ganodermanontriol Suppresses the Progression of Lung Adenocarcinoma by Activating CES2 to Enhance the Metabolism of Mycophenolate Mofetil., PMID:38419324
In Silico Study of Camptothecin-Based Pro-Drugs Binding to Human Carboxylesterase 2., PMID:38397391
Variability in plasma rifampicin concentrations and role of SLCO1B1, ABCB1, AADAC2 and CES2 genotypes in Ethiopian patients with tuberculosis., PMID:38315168
Establishment of human intestinal organoids derived from commercially available cryopreserved intestinal epithelium and evaluation for pharmacokinetic study., PMID:38064926
Evaluation of potential targets to enhance the sensitivity of cholangiocarcinoma cells to anticancer drugs., PMID:37832404
Comparative Intra-Subject Analysis of Gene Expression and Protein Abundance of Major and Minor Drug Metabolizing Enzymes in Healthy Human Jejunum and Liver., PMID:37739780
Irinotecan-Induced Toxicity: A Pharmacogenetic Study Beyond UGT1A1., PMID:37715926
Evaluation of Human Hepatocyte Drug Metabolism Carrying High-Risk or Protection-Associated Liver Disease Genetic Variants., PMID:37686209
Quantification of drug metabolising enzymes and transporter proteins in the paediatric duodenum via LC-MS/MS proteomics using a QconCAT technique., PMID:37625656
Human Carboxylesterase 1A Plays a Predominant Role in Hydrolysis of the Anti-Dyslipidemia Agent Fenofibrate in Humans., PMID:37550069
New bysspectin A derivatives as potent inhibitors of human carboxylesterase 2A., PMID:37544184
Effects of different stocking density and various phytogenic feed additives dosage levels on growing-finishing pigs., PMID:37332281
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea., PMID:37300962
Organism-wide, cell-type-specific secretome mapping of exercise training in mice., PMID:37141889
5-ALA treatment increases intracellular heme levels and enhances CYP3A4 activity in genome-edited Caco-2 cells., PMID:37141642
Carboxylesterase 2a deletion provokes hepatic steatosis and insulin resistance in mice involving impaired diacylglycerol and lysophosphatidylcholine catabolism., PMID:37059417
Generation of Caco-2 cells with predictable metabolism by CYP3A4, UGT1A1 and CES using the PITCh system., PMID:37037169
Design and Construction of Carboxylesterase 2c Gene Knockout Rats by CRISPR/Cas9., PMID:36694315
Rational design of a turn-on near-infrared fluorescence probe for the highly sensitive and selective monitoring of carboxylesterase 2 in living systems., PMID:36661088
Evaluation on the Metabolic Activity of Two Carboxylesterase Isozymes in Mouse Liver Microsomes by a LC-MS/MS Method., PMID:36585777
Vicagrel is hydrolyzed by Raf kinase inhibitor protein in human intestine., PMID:36519186
The Crystal Structure of Mouse Ces2c, a Potential Ortholog of Human CES2, Shows Structural Similarities in Substrate Regulation and Product Release to Human CES1., PMID:36361897
Impact of pregnancy related hormones on drug metabolizing enzyme and transport protein concentrations in human hepatocytes., PMID:36210832
Activity and Expression of Carboxylesterases and Arylacetamide Deacetylase in Human Ocular Tissues., PMID:36195336
For research use only. Not for human or drug use.
