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Teriparatide ELISA Kit

Catalog #:   KDB93401 Specific References (50) DATASHEET
Applications: Used for the quantitative determination of Teriparatide concentration in serum and plasma.
Sample type: Plasma, Serum
Sensitivity: 1.16 ng/mL
Range: 1.25 - 80 ng/mL
Overview

Catalog No.

KDB93401

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. An antibody specific for Teriparatide has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Teriparatide and then pipetted into the wells. Teriparatide in the sample competitively binds to the pre-coated antibody with biotin-labeled Teriparatide. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Teriparatide bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Teriparatide concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

1.25 - 80 ng/mL

Sensitivity

1.16 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

 

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

16

16

16

24

24

24

Mean (ng/mL)

63.8

6.4

3.8

64.9

6.1

3.7

Standard deviation

7.5

0.2

0.3

7.6

0.6

0.4

CV (%)

11.8

3.8

8.4

11.7

10.2

11.5

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Data Image
  • Experiment Example
    CALCULATION OF RESULTS
    Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. Do not include the blank in the standard curve. The data may be linearized by plotting the log of the Teriparatide concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
References

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Identification of 4'-Thiouridine as an Orally Available Antiviral Agent Targeting Both RdRp and NiRAN Functions of SARS-CoV-2 Nsp12., PMID:40512093

Associations and Interactions Between Neuroticism, Adverse Life Events and Health Anxiety: Results From a Large Representative Cohort., PMID:40510544

Optimization of Textural and Structural Properties of Carbon Materials for Sodium Dual-Ion Battery Electrodes., PMID:40509326

Minimizing human-induced variability in quantitative angiography for a robust and explainable AI-based occlusion prediction in flow diverter-treated aneurysms., PMID:40506221

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Remission conversion drives outcomes after CAR T-cell therapy for multiple myeloma: a registry analysis from the DRST., PMID:40504993

Risk Factors and Consequences of Cage Subsidence after Single-level Posterior or Transforaminal Lumbar Interbody Fusion: A Retrospective Multicenter Study., PMID:40503199

Insulin-Degrading Enzyme Regulates mRNA Processing and May Interact with the CCR4-NOT Complex., PMID:40497968

Emerging therapies for HBsAg seroclearance: spotlight on novel combination strategies., PMID:40495020

C-terminal binding protein 2 interacts with JUNB to control macrophage inflammation., PMID:40490364

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Asymptomatic sigmoid diverticulum perforation-induced subcutaneous, mediastinal, and retroperitoneal emphysema: A case report and literature review., PMID:40476087

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High HBsAg Clearance Rate and Viral Dynamics in HBeAg-Positive, ALT-Normal Children and Adolescents with Chronic HBV Infection: Results from the Prospective Sprout Project., PMID:40469009

Iron status at early pregnancy is associated with infectious respiratory and gastric illness in women receiving routine iron supplementation: the NuPED prospective cohort., PMID:40468238

FDA Approval Summary: Idecabtagene Vicleucel for the Treatment of Triple-Class Exposed, Relapsed or Refractory Multiple Myeloma., PMID:40465403

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The glymphatic system in oncology: from the perspective of a radiation oncologist., PMID:40460451

Targeting FLT3 Mutations in Acute Myeloid Leukemia: The Role of Quizartinib in Precision Medicine., PMID:40456703

Knowledge and Awareness of Generative Artificial Intelligence Use in Medicine Among International Stakeholders: A Cross-Sectional Study., PMID:40455490

Capsule-Preserving Intraocular Lens Intrascleral Fixation for Zonular Weakness: Clinical Outcomes and Comparative Analysis., PMID:40454421

Decreased risk of nosocomial transmission of hepatitis B and C viruses among hemodialysis patients in Southern Bulgaria., PMID:40452540

Correction: Nucleos(t)ide analogs continuation is not associated with a lower risk of HBsAg seroreversion following PEG-IFN-induced HBsAg loss., PMID:40450325

Association Between Insulin Resistance and Incomplete Endothelization of LAAC Devices in Patients with Atrial Fibrillation: A Retrospective Study., PMID:40450175

Decreased expression of insulin-degrading enzyme increases gluconeogenesis and glucose production in cultured hepatocytes administered with glucagon., PMID:40450129

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Preoperative Proteinuria is Associated with Higher Mortality After Fenestrated/Branched Aortic Repair of Complex Anatomy Aortic Aneurysms., PMID:40447170

Validation of the peripherally inserted central venous catheter insertion length prediction formula using height in children., PMID:40444548

Effect of Nucleos(t)ide Analogues Withdrawal on Survival Outcomes in Patients With Hepatitis B Virus-Related Acute-on-Chronic Liver Failure., PMID:40443167

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Clinical significance of computer-aided quality assessment systems in colonoscopy: a comprehensive review., PMID:40438910

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Isolation and Characterization of β-Phenylethylamine-Producing Lactic Acid Bacteria from Dairy Products., PMID:40431140

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Effects of Combination Treatment with Leptin and Liraglutide on Glucose Metabolism in Insulin-Dependent Diabetic Mice., PMID:40429740

HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide (Leuciscus waleckii) Under Extreme Alkaline Conditions., PMID:40427687

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Datasheet

Document Download

Teriparatide ELISA Kit.pdf

 

$ 1128
Product specifications
96 T 1128

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Teriparatide ELISA Kit [KDB93401]
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