Catalog No.
YXX23601
Expression system
E. coli
Species
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Protein length
Met1-Asp471
Predicted molecular weight
55.06 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q7ZAK5
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Pneumolysin, PLY, Thiol-activated cytolysin, ply, spr1739
Pneumococcal surface proteins as targets for next-generation vaccines: Addressing the challenges of serotype variation., PMID:40347702
Immunochemical Characteristics and Functional Activity of Monoclonal Antibodies Obtained to the Recombinant Form of Pneumolysin., PMID:39945949
Structural analysis of extracellular ATP-independent chaperones of streptococcal species and protein substrate interactions., PMID:39878509
Weakened Airway Epithelial Junctions and Enhanced Neutrophil Elastase Release Contribute to Age-Dependent Bacteremia Risk Following Pneumococcal Pneumonia., PMID:39778043
Pneumococcal pneumonia is driven by increased bacterial turnover due to bacteriocin-mediated intra-strain competition., PMID:39638898
Endothelial ENaC-α Restrains Oxidative Stress in Lung Capillaries in Murine Pneumococcal Pneumonia-associated Acute Lung Injury., PMID:39405473
Forsythoside B, the active component of Frosythiae fructuse water extract, alleviates Streptococcus pneumoniae virulence by targeting pneumolysin., PMID:39366754
Pneumolysin contributes to dysfunction of nasal epithelial barrier for promotion of pneumococcal dissemination into brain tissue., PMID:39345124
Pneumolysin-responsive liposomal platform for selective treatment of Streptococcus pneumoniae., PMID:39285123
A 3'UTR-derived small RNA represses pneumolysin synthesis and facilitates pneumococcal brain invasion., PMID:39271946
Molecular characterization of a novel putative pathogen, Streptococcus nakanoensis sp. nov., isolated from sputum culture., PMID:39269180
Cytokine secretion by in vitro cultures of lung epithelial cells, differentiated macrophages and differentiated dendritic cells incubated with pneumococci and pneumococcal extracellular vesicles., PMID:39254798
Microenvironmental acidification by pneumococcal sugar consumption fosters barrier disruption and immune suppression in the human alveolus., PMID:39231629
Bacterial pore-forming toxin pneumolysin drives pathogenicity through host extracellular vesicles released during infection., PMID:39211544
Preliminary Evaluation of the Safety and Immunogenicity of a Novel Protein-Based Pneumococcal Vaccine in Healthy Adults Aged 18-49: A Phase Ia Randomized, Double Blind, Placebo-Controlled Clinical Study., PMID:39203953
Mucins protect against Streptococcus pneumoniae virulence by suppressing pneumolysin expression., PMID:39172520
Blocking HXA3-mediated neutrophil elastase release during S. pneumoniae lung infection limits pulmonary epithelial barrier disruption and bacteremia., PMID:39120139
From cytokines to chemokines: Understanding inflammatory signaling in bacterial meningitis., PMID:39116800
Analysis of Immunobiological Properties of Recombinant Streptococcus pneumoniae Pneumolysin., PMID:39090461
Pneumococcal Neuraminidases Increase Platelet Killing by Pneumolysin., PMID:39029905
Analysis of Toxicity of Recombinant Pneumolysin from Streptococcus pneumoniae., PMID:38960960
Glucose and Oxygen Levels Modulate the Pore-Forming Effects of Cholesterol-Dependent Cytolysin Pneumolysin from Streptococcus pneumoniae., PMID:38922127
Targeted small molecule inhibitors blocking the cytolytic effects of pneumolysin and homologous toxins., PMID:38670939
Klebsiella pneumoniae peptide hijacks a Streptococcus pneumoniae permease to subvert pneumococcal growth and colonization., PMID:38589539
Pneumococcal sialidase promotes bacterial survival by fine-tuning of pneumolysin-mediated membrane disruption., PMID:38483905
Estimates of differential toxin expression governing heterogeneous intracellular lifespans of Streptococcus pneumoniae., PMID:38411297
Influence of the mRNA initial region on protein production: a case study using recombinant detoxified pneumolysin as a model., PMID:38260740
Recombinant Pneumolysin of Pneumococci Induces TLR4 Expression and Maturation of Dendritic Cells In Vitro., PMID:38191877
Fusion of PspA to detoxified pneumolysin enhances pneumococcal vaccine coverage., PMID:38096222
Pericytes are protective in experimental pneumococcal meningitis through regulating leukocyte infiltration and blood-brain barrier function., PMID:37978545
Impact of Endogenous Pneumococcal Hydrogen Peroxide on the Activity and Release of Pneumolysin., PMID:37888624
Isorhamnetin as a novel inhibitor of pneumolysin against Streptococcus pneumoniae infection in vivo/in vitro., PMID:37839759
Recent progress in pneumococcal protein vaccines., PMID:37818378
Pore-forming activity of S. pneumoniae pneumolysin disrupts the paracellular localization of the epithelial adherens junction protein E-cadherin., PMID:37607057
ΔA146Ply-HA stem protein immunization protects mice against influenza A virus infection and co-infection with Streptococcus pneumoniae., PMID:37531919
The Global Burden of Community-Acquired Pneumonia in Adults, Encompassing Invasive Pneumococcal Disease and the Prevalence of Its Associated Cardiovascular Events, with a Focus on Pneumolysin and Macrolide Antibiotics in Pathogenesis and Therapy., PMID:37446214
The unremarkable alveolar epithelial glycocalyx: a thorium dioxide-based electron microscopic comparison after heparinase or pneumolysin treatment., PMID:37386200
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids., PMID:37348647
Macrolides Decrease the Proinflammatory Activity of Macrolide-Resistant Streptococcus pneumoniae., PMID:37191519
Preparation a Recombinant Form of Pneumolysin Protein from Streptococcus pneumoniae., PMID:37160796
Pneumolysin as a target for new therapies against pneumococcal infections: A systematic review., PMID:36947540
Naturally-occurring serotype 3 Streptococcus pneumoniae strains that lack functional pneumolysin and autolysin have attenuated virulence but induce localized protective immune responses., PMID:36897919
Bacteria hijack a meningeal neuroimmune axis to facilitate brain invasion., PMID:36859544
Immunoinformatics-aided design of a new multi-epitope vaccine adjuvanted with domain 4 of pneumolysin against Streptococcus pneumoniae strains., PMID:36829109
Peptide linker increased the stability of pneumococcal fusion protein vaccine candidate., PMID:36777254
Wogonin attenuates the pathogenicity of Streptococcus pneumoniae by double-target inhibition of Pneumolysin and Sortase A., PMID:36747468
Dendritic spine loss deep in the neocortex and dendrite distortion with diffusion disturbances occur early in experimental pneumococcal meningitis., PMID:36704002
Meningitis-associated pneumococcal serotype 8, ST 53, strain is hypervirulent in a rat model and has non-haemolytic pneumolysin which can be attenuated by liposomes., PMID:36683678
Pneumolysin promotes host cell necroptosis and bacterial competence during pneumococcal meningitis as shown by whole-animal dual RNA-seq., PMID:36543127
A multiomics analysis of direct interkingdom dynamics between influenza A virus and Streptococcus pneumoniae uncovers host-independent changes to bacterial virulence fitness., PMID:36542660