Catalog No.
PXX01301
Species reactivity
Clostridium perfringens (strain 13 / Type A)
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Asp30-Asn500).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:4000
Target
Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163
Concentration
0.9 mg/ml
Purification
Purified by antigen affinity column.
Accession
P0C2E9
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Immunogenic and neutralization efficacy of recombinant perfringolysin O of Clostridium perfringens and its C-terminal receptor-binding domain in a murine model., PMID:35032316
An anti-perfringolysin O monoclonal antibody cross-reactive with streptolysin O protects against streptococcal toxic shock syndrome., PMID:32891180
Non-toxic perfringolysin O and α-toxin derivatives as potential vaccine candidates against bovine necrohaemorrhagic enteritis., PMID:27810219
Antibody-mediated neutralization of perfringolysin o for intracellular protein delivery., PMID:25881713
Visualization of cholesterol deposits in lysosomes of Niemann-Pick type C fibroblasts using recombinant perfringolysin O., PMID:24775609
Perfringolysin O as a useful tool to study human sperm physiology., PMID:22999796
Beta toxin is essential for the intestinal virulence of Clostridium perfringens type C disease isolate CN3685 in a rabbit ileal loop model., PMID:18078439
Assembly and topography of the prepore complex in cholesterol-dependent cytolysins., PMID:12777381