Catalog No.
PHH34301
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human HIF1A (Asp55-Val342).
Applications
ELISA, IHC, IF, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:40000, IHC: 1:100-1:300, IF:1:50-1:200, WB: 1:500-1:2000 |
Target
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
Concentration
1 mg/ml
Purification
Purified by antigen affinity column.
Accession
Q16665
Form
Liquid
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Changes in colostrum ingredients of Hu sheep, as well as the missense mutation genes associated with colostrum yield., PMID:35196466
Hypoxia-induced interstitial transformation of microvascular endothelial cells by mediating HIF-1α/VEGF signaling in systemic sclerosis., PMID:35231032
Targeting HIF-1α abrogates PD-L1-mediated immune evasion in tumor microenvironment but promotes tolerance in normal tissues., PMID:35239514
The CD146-HIF-1α axis regulates epithelial cell migration and alveolar maturation in a mouse model of bronchopulmonary dysplasia., PMID:35306530
Human organ rejuvenation by VEGF-A: Lessons from the skin., PMID:35749494
HNRNPC downregulation inhibits IL-6/STAT3-mediated HCC metastasis by decreasing HIF1A expression., PMID:35848884
CRMP2 derived from cancer associated fibroblasts facilitates progression of ovarian cancer via HIF-1α-glycolysis signaling pathway., PMID:35927239
Cyclosporine A Modulates LSP1 Protein Levels in Human B Cells to Attenuate B Cell Migration at Low O2 Levels., PMID:36013463
A novel regulated network mediated by downregulation HIF1A-AS2 lncRNA impairs placental angiogenesis by promoting ANGPTL4 expression in preeclampsia., PMID:36016656
GDF11 promotes wound healing in diabetic mice via stimulating HIF-1ɑ-VEGF/SDF-1ɑ-mediated endothelial progenitor cell mobilization and neovascularization., PMID:36347996
Heme oxygenase-1 protects against PM2.5 induced endothelial dysfunction through inhibition of HIF1α., PMID:36427673
Doxorubicin induced ROS-dependent HIF1α activation mediates blockage of IGF1R survival signaling by IGFBP3 promotes cardiac apoptosis., PMID:36602546
Newly identified form of phenotypic plasticity of cancer: immunogenic mimicry., PMID:36754910
Manassantin A inhibits tumour growth under hypoxia through the activation of chaperone-mediated autophagy by modulating Hsp90 activity., PMID:36759727
LncRNA FRMD6-AS1 promotes hepatocellular carcinoma cell migration and stemness by regulating SENP1/HIF-1α axis., PMID:36827886
IGF2BP3/HIF1A/YAP signaling plays a role in driving acute-on-chronic liver failure through activating hepatocyte reprogramming., PMID:37257765
HIF-1α/MMP-9 promotes spinal cord central sensitization in rats with bone cancer pain., PMID:37356787
In Silico Evaluation of HN-N07 Small Molecule as an Inhibitor of Angiogenesis and Lymphangiogenesis Oncogenic Signatures in Non-Small Cell Lung Cancer., PMID:37509650
Analysis of microRNA expression in rat kidneys after VEGF inhibitor treatment under different degrees of hypoxia., PMID:37642276
MT1-MMP as a Key Regulator of Metastasis., PMID:37681919
MCPIP-1 knockdown enhances endothelial colony-forming cell angiogenesis via the TFRC/AKT/mTOR signaling pathway in the ischemic penumbra of MCAO mice., PMID:37689231
Unrevealed roles of extracellular enolase‑1 (ENO1) in promoting glycolysis and pro‑cancer activities in multiple myeloma via hypoxia‑inducible factor 1α., PMID:37800625
Hypoxia-induced activation of NDR2 underlies brain metastases from Non-Small Cell Lung Cancer., PMID:38092743
Soluble CD83 modulates human-monocyte-derived macrophages toward alternative phenotype, function, and metabolism., PMID:38162675
Gp130-HIF1α axis-induced vascular damage is prevented by the short-term inhibition of IL-6 receptor signaling., PMID:38165930
Effect of hypoxia-inducible factor-1 alpha expression on survival in patients with metastatic cervical squamous cell carcinoma treated with first-line chemotherapy and bevacizumab., PMID:38447002
CircHIF1A induces cetuximab resistance in colorectal cancer by promoting HIF1α-mediated glycometabolism alteration., PMID:38715141
Polyphyllin I ameliorates gefitinib resistance and inhibits the VEGF/VEGFR2/p38 pathway by targeting HIF-1a in lung adenocarcinoma., PMID:38761523
Endothelial HIF2α suppresses retinal angiogenesis in neonatal mice by upregulating NOTCH signaling., PMID:38770916
Epstein-Barr virus causes vascular abnormalities in epithelial malignancies through upregulating ANXA3-HIF-1α-VEGF pathway., PMID:38778160
Ze-Qi-Tang formula inhibits MDSCs glycolysis through the down-regulation of p21/Hif1α/Glut1 signal in psoriatic-like mice., PMID:38810554
Exploration of the Shared Gene Signatures and Molecular Mechanisms between Chronic Bronchitis and Antineutrophil Cytoplasmic Antibody-associated Glomerulonephritis: Evidence from Transcriptome Data., PMID:38847168
CAFs-Associated Genes (CAFGs) in Pancreatic Ductal Adenocarcinoma (PDAC) and Novel Therapeutic Strategy., PMID:38892190
Antioxidant effect of gallic acid on retinal ganglion cells in glaucoma model., PMID:38942959
A Targetable Secreted Neural Protein Drives Pancreatic Cancer Metastatic Colonization and HIF1α Nuclear Retention., PMID:39028915
Helicobacter pylori upregulates PAD4 expression via stabilising HIF-1α to exacerbate rheumatoid arthritis., PMID:39107082
MOGAT3-mediated DAG accumulation drives acquired resistance to anti-BRAF/anti-EGFR therapy in BRAFV600E-mutant metastatic colorectal cancer., PMID:39436710
Anti-VEGFR2 neutralising antibody slows the progression of multistep oral carcinogenesis., PMID:39462847
Alleviating Tumor Hypoxia and Immunosuppression via Sononeoperfusion: A New Ally for potentiating anti-PD-L1 blockade of solid Tumor., PMID:39482116
Inhibition of Bruton's tyrosine kinase with PD-1 blockade modulates T cell activation in solid tumors., PMID:39513363
Metabolic rewiring controlled by HIF-1α tunes IgA-producing B-cell differentiation and intestinal inflammation., PMID:39543372
ITGA3 promotes pancreatic cancer progression through HIF1α- and c-Myc-driven glycolysis in a collagen I-dependent autocrine manner., PMID:39690180
Oral exposure to ovalbumin alters glucose metabolism in sensitized mice: upregulation of HIF-1α-mediated glycolysis., PMID:39711353
Impact of anti-PD1 immunotherapy and circulating tumor cells on progression-free survival in surgical pancreatic adenocarcinoma: a retrospective cohort study., PMID:39745065
Upshot of Some Bioactive Compounds on Angiogenesis in Retinal Pigment Epithelial Cells., PMID:39753522
Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitor, FG4592, Induces Endogenous Metallothionein3 Expression in Human Neuronal Cell Line, ReNcell CX Cells., PMID:39993744
Hypoxia inducible factor-1α drives cancer resistance to cuproptosis., PMID:40054467
Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis., PMID:40055343
Cetuximab and Paclitaxel Drug Response in Head and Neck Tumor Stem Cells., PMID:40149887
Immunodynamic axis of fibroblast-driven neutrophil infiltration in acute pancreatitis: NF-κB-HIF-1α-CXCL1., PMID:40335899
For research use only. Not for human or drug use.
