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Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody

Catalog #:   PHF37501 Specific References (32) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P60709
Overview

Catalog No.

PHF37501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human β-actin (Met1-Phe375).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Beta-actin, ACTB, Actin, cytoplasmic 1

Purification

Purified by antigen affinity column.

Accession

P60709

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with β-actin antibody (PHF37501) at 1:3000.

    Lane 1: Zebra fish lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse kidney lysate
    Lane 4: Mouse stomach lysate
    Lane 5: Mouse lung lysate
    Lane 6: BGC cell lysate
    Lane 7: TPC1 cell lysate
    Lane 8: A549 cell lysate
    Lane 9: Human gastric carcinoma lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 45 kDa
    Observed MW: 45 kDa
References

Si Microanemones Integrated Microfluidic Chip for Highly Efficient Isolation of Extracellular Vesicles., PMID:40395100

Exploring heterogeneous expression of beta-actin (ACTB) in bladder cancer by producing a monoclonal antibody 6D6., PMID:38867273

Proteomic Analysis of Egg Yolk Proteins During Embryonic Development in Wanxi White Goose., PMID:38433387

Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair., PMID:37978868

Alterations in the expression pattern of RBC membrane associated proteins (RMAPs) in whole body γ-irradiated Sprague Dawley rats., PMID:37315317

Porcine reproductive and respiratory syndrome virus degrades DDX10 via SQSTM1/p62-dependent selective autophagy to antagonize its antiviral activity., PMID:36779599

Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells., PMID:35899934

Low anti-CFL1 antibody with high anti-ACTB antibody is a poor prognostic factor in esophageal squamous cell carcinoma., PMID:35780443

Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis., PMID:35491858

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD., PMID:35473441

Routine noninvasive prenatal screening for fetal Rh D in maternal plasma-A 2-year experience from a single center in Belgium., PMID:35352833

Circulating Mir-140 and leptin improve the accuracy of the differential diagnosis between psoriatic arthritis and rheumatoid arthritis: a case-control study., PMID:34380068

Novel serum autoantibodies against ß-actin (ACTB) in amyotrophic lateral sclerosis., PMID:33792444

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

TFEB rearranged renal cell carcinoma. A clinicopathologic and molecular study of 13 cases. Tumors harboring MALAT1-TFEB, ACTB-TFEB, and the novel NEAT1-TFEB translocations constantly express PDL1., PMID:33208882

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545

Identification of miR-200a-5p targeting the peptide transporter TAP1 and its association with the clinical outcome of melanoma patients., PMID:32923135

The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses., PMID:32249716

Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation., PMID:32019420

Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease., PMID:31462148

Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits., PMID:31234698

ATG5 is required for B cell polarization and presentation of particulate antigens., PMID:30196744

Optimization of Recovery and Analysis of RNA in Sorted Cells in mRNA Quantification After Fluorescence-activated Cell Sorting., PMID:27993868

Clinical relevance of miR-mediated HLA-G regulation and the associated immune cell infiltration in renal cell carcinoma., PMID:26155421

Targeting syndecan-1 in breast cancer inhibits osteoclast functions through up-regulation of osteoprotegerin., PMID:26909290

DDX6 post-transcriptionally down-regulates miR-143/145 expression through host gene NCR143/145 in cancer cells., PMID:23932921

Env gene expression of human endogenous retrovirus-k and human endogenous retrovirus-w in childhood acute leukemia cells., PMID:23328642

Immunization with ovarian autoantigens leads to reduced fertility in mice following follicular dysfunction., PMID:22143970

Identification and validation of candidate biomarkers involved in human ovarian autoimmunity., PMID:21890413

Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids., PMID:20477829

An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV., PMID:20398263

Characterizing mouse male germ cell-specific actin capping protein alpha3 (CPalpha3): dynamic patterns of expression in testicular and epididymal sperm., PMID:18645673

Datasheet
$ 130
Product specifications
50 μg 130 100 μg 220

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For research use only. Not for human or drug use.

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Anti-ACTB/β-actin/Beta Actin Polyclonal Antibody [PHF37501]
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