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Recombinant HCMV/HHV-5 pp65/UL83 Protein, N-GST

Catalog #:   YVV37201 Specific References (50) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: P06725
Protein length: Thr297-Tyr510
Overview

Catalog No.

YVV37201

Expression system

E. coli

Species

Human cytomegalovirus (HCMV/HHV-5)

Protein length

Thr297-Tyr510

Predicted molecular weight

49.85 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

P06725

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

65 kDa phosphoprotein, pp65, 65 kDa matrix phosphoprotein, Phosphoprotein UL83, Tegument protein UL83, UL83

Data Image
  • SDS-PAGE
    SDS-PAGE for Recombinant Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) pp65/UL83 protein
References

Performance of new pp65-IGRA for the quantification of HCMV-specific CD4+ T-cell response in healthy subjects and in solid organ transplant recipients., PMID:40443680

The guinea pig as a relevant preclinical model in the rat race for a vaccine against congenital cytomegalovirus infection., PMID:40413831

Human Cytomegalovirus Antigen Presentation by HLA-G in Infected Cells., PMID:40347012

Pre-transplant IE1-specific T-cell response and CD8+ T-cell count as predictive markers of treated HCMV reactivation in kidney transplant recipients., PMID:40308605

A promising endeavor against human cytomegalovirus: Predominant epitopes-based recombinant subunit vaccine RHEcIE1/pp65/pp150., PMID:40277436

Protein-S-nitrosylation of human cytomegalovirus pp65 reduces its ability to undermine cGAS., PMID:40243337

Integrating clinical data and genetic susceptibility to elucidate the relationship between systemic lupus erythematosus and human cytomegalovirus infection., PMID:39614342

Different Antigen-Specific CD4+ and CD8+ T-Cell Response against HCMV Proteins in Pregnant Women with Primary Infection and in Control Subjects with Remote Infection., PMID:39336935

T cell inducing vaccine against cytomegalovirus immediate early 1 (IE1) protein provides high level cross strain protection against congenital CMV., PMID:39298998

Immune Control of Human Cytomegalovirus (HCMV) Infection in HCMV-Seropositive Solid Organ Transplant Recipients: The Predictive Role of Different Immunological Assays., PMID:39195215

Dynamic monitoring of viral gene expression reveals rapid antiviral effects of CD8 T cells recognizing the HCMV-pp65 antigen., PMID:39104541

Cytomegalovirus-induced peroxynitrite promotes virus entry and contributes to pathogenesis in a murine model of infection., PMID:38953361

Performance and Stability of New Class of Fetal Bovine Sera (FBS) and Its Lyophilized Form in ELISpot and FluoroSpot Assays: Applications for Monitoring the Immune Response in Vaccine, and Cell and Gene Immunotherapy in Clinical Trials., PMID:38502401

Frequencies of activated T cell populations increase in breast milk of HCMV-seropositive mothers during local HCMV reactivation., PMID:38235135

Re-Evaluating Human Cytomegalovirus Vaccine Design: Prediction of T Cell Epitopes., PMID:38005961

Non-classical HLA-E restricted CMV 15-mer peptides are recognized by adaptive NK cells and induce memory responses., PMID:37809084

Human cytomegalovirus infection enhances 5‑lipoxygenase and cycloxygenase‑2 expression in colorectal cancer., PMID:37654195

Integrated immune monitoring of HCMV infection in pregnant women with complications and its association with adverse pregnancy outcomes., PMID:37040845

Persistent CD8 T Cell Marks Caused by the HCMV Infection in Seropositive Adults: Prevalence of HLA-E-Reactive CD8 T Cells., PMID:36980230

CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses., PMID:36723437

ELISPOT assays with pp65 peptides or whole HCMV antigen are reliable predictors of immune control of HCMV infection in seropositive kidney transplant recipients., PMID:36655741

Distinctive phenotype for HLA-E- versus HLA-A2-restricted memory CD8 αβT cells in the course of HCMV infection discloses features shared with NKG2C+CD57+NK and δ2-γδT cell subsets., PMID:36532017

Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein., PMID:36000848

Viral and Cellular Factors Contributing to the Hematogenous Dissemination of Human Cytomegalovirus via Polymorphonuclear Leukocytes., PMID:35891541

Development and Characterization of an HCMV Multi-Antigen Therapeutic Vaccine for Glioblastoma Using the UNITE Platform., PMID:35651802

Sequencing of the Viral UL111a Gene Directly from Clinical Specimens Reveals Variants of HCMV-Encoded IL-10 That Are Associated with Altered Immune Responses to HCMV., PMID:35563032

Horizontal Transmission of Cytomegalovirus in a Rhesus Model Despite High-Level, Vaccine-Elicited Neutralizing Antibody and T-Cell Responses., PMID:35413121

Mapping and Characterization of HCMV-Specific Unconventional HLA-E-Restricted CD8 T Cell Populations and Associated NK and T Cell Responses Using HLA/Peptide Tetramers and Spectral Flow Cytometry., PMID:35008688

Therapeutic Vaccination of Hematopoietic Cell Transplantation Recipients Improves Protective CD8 T-Cell Immunotherapy of Cytomegalovirus Infection., PMID:34489940

A conditionally replication-defective cytomegalovirus vaccine elicits potent and diverse functional monoclonal antibodies in a phase I clinical trial., PMID:34078915

ELISPOT assay of interferon-γ secretion for evaluating human cytomegalovirus reactivation risk in allo-HSCT recipients., PMID:34076905

Surface NKG2C Identifies Differentiated αβT-Cell Clones Expanded in Peripheral Blood., PMID:33664730

Guinea pig cytomegalovirus protective T cell antigen GP83 is a functional pp65 homolog for innate immune evasion and pentamer dependent virus tropism., PMID:33658350

Discordance Between the Predicted Versus the Actually Recognized CD8+ T Cell Epitopes of HCMV pp65 Antigen and Aleatory Epitope Dominance., PMID:33633736

Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly., PMID:33504601

The Autophagy-Initiating Protein Kinase ULK1 Phosphorylates Human Cytomegalovirus Tegument Protein pp28 and Regulates Efficient Virus Release., PMID:33328309

Detection of human cytomegalovirus in synovial neutrophils obtained from patients with rheumatoid arthritis., PMID:33243069

Evidence of human cytomegalovirus infection and expression of 5-lipoxygenase in borderline ovarian tumors., PMID:33174621

A simple and efficient method of generating HCMV pp65-specific T cells using overlapping peptides., PMID:33151741

Substitution-inert polynuclear platinum compounds inhibit human cytomegalovirus attachment and entry., PMID:33132195

Public and private human T-cell clones respond differentially to HCMV antigen when boosted by CD3 copotentiation., PMID:33125463

Antigenic competition in the generation of multi-virus-specific cell lines for immunotherapy of human cytomegalovirus, polyomavirus BK, Epstein-Barr virus and adenovirus infection in haematopoietic stem cell transplant recipients., PMID:33031870

Human cytomegalovirus DNA detection in a recurrent glioblastoma multiforme tumour, but not in whole blood: a case report and discussion about the HCMV latency and therapy perspectives., PMID:32880872

A study of antigen-specific anti-cytomegalovirus antibody reactivity in patients with systemic sclerosis and concomitant anti-Ro52 antibodies., PMID:32681396

Assessing Anti-HCMV Cell Mediated Immune Responses in Transplant Recipients and Healthy Controls Using a Novel Functional Assay., PMID:32670891

Evaluation of T-activated proteins as recall antigens to monitor Epstein-Barr virus and human cytomegalovirus-specific T cells in a clinical trial setting., PMID:32552697

Human cytomegalovirus pp65 peptide-induced autoantibodies cross-reacts with TAF9 protein and induces lupus-like autoimmunity in BALB/c mice., PMID:32541894

Detection of human cytomegalovirus in patients with epithelial ovarian cancer and its impacts on survival., PMID:32322296

Human cytomegalovirus IE2 drives transcription initiation from a select subset of late infection viral promoters by host RNA polymerase II., PMID:32251483

Human Cytomegalovirus Infection Induces High Expression of Prolactin and Prolactin Receptors in Ovarian Cancer., PMID:32121009

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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For research use only. Not for human or drug use.

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Recombinant HCMV/HHV-5 pp65/UL83 Protein, N-GST [YVV37201]
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