Catalog No.
KAH72401
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Tiragolumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Tiragolumab will be captured by immobilized Tiragolumab. After washing away any unbound substances, a biotin-labeled Tiragolumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Tiragolumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Tiragolumab concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
156.25 - 10,000 ng/mL
Sensitivity
10.11 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
5152.6 |
1166.1 |
282.1 |
5149.7 |
1342.1 |
325.3 |
|
Standard deviation |
127.7 |
22.2 |
8.8 |
175.2 |
43.1 |
18.0 |
|
CV (%) |
2.5 |
1.9 |
3.1 |
3.4 |
3.2 |
5.5 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Alternative Names
MTIG-7192-A, MTIG-7192A, MTIG7192A, RG-6058, RO-7092284, RO7092284,CAS: 1918185-84-8
For research use only. Not for human or drug use.
