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Anti-MPXV A42R/Profilin Polyclonal Antibody (PVV21901)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:Q8V4T7
Overview

Catalog No.

PVV21901

Species reactivity

Monkeypox virus (strain Zaire-96-I-16) (MPX)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant MPXV A42R/Profilin (Met1-Asn133).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:4000

Target

Profilin, OPG171, A42R

Concentration

1.98 mg/ml

Purification

Purified by antigen affinity column.

Accession

Q8V4T7

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with A42R/Profilin antibody (PVV21901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 17 kDa
    Observed MW: 17 kDa
References

Identification of Antiviral Compounds against Monkeypox Virus Profilin-like Protein A42R from Plantago lanceolata. PMID: 36431817

Structure of the Monkeypox virus profilin-like protein A42R reveals potential functional differences from cellular profilins. PMID: 36189721

Tissue-specific expression of profilin. PMID: 2721687

Characterization of profilin and polcalcin panallergens from ash pollen. PMID: 25219108

Identification of oilseed rape (Brassica napus) pollen profilin as a cross-reactive allergen. PMID: 14600423

Peach profilin: cloning, heterologous expression and cross-reactivity with Bet v 2. PMID: 12823124

Characterization and localization of profilin in pollen grains and tubes of Lilium longiflorum. PMID: 9096955

Specific immune response to Phleum pratense plant profilin in atopic patients and control subjects. PMID: 11449535

Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: isolation, amino acid sequences, and immunologic properties. PMID: 15208604

In vivo MR and Fluorescence Dual-modality Imaging of Atherosclerosis Characteristics in Mice Using Profilin-1 Targeted Magnetic Nanoparticles. PMID: 26877785

Expression analysis and characteristics of profilin gene from silkworm, Bombyx mori. PMID: 18633732

Novel murine mAbs define specific and cross-reactive epitopes on the latex profilin panallergen Hev b 8. PMID: 33045539

Distribution of profilin in fibroblasts correlates with the presence of highly dynamic actin filaments. PMID: 1581979

Bell peppers (Capsicum annuum) express allergens (profilin, pathogenesis-related protein P23 and Bet v 1) depending on the horticultural strain. PMID: 9652302

Location of profilin at presynaptic sites in the cerebellar cortex; implication for the regulation of the actin-polymerization state during axonal elongation and synaptogenesis. PMID: 8106880

Trypanosoma cruzi actins: Expression analysis of actin 2. PMID: 30961931

Sequence homology: a poor predictive value for profilins cross-reactivity. PMID: 16153305

Specific immune response to Parietaria judaica plant profilin: a low T cell proliferative response supports high IgE and skin prick test. PMID: 11958736

Purification and characterization of an allergen from celery immunochemically related to an allergen present in several other plant species. Identification as a profilin. PMID: 1525697

The EGF receptor is an actin-binding protein. PMID: 1383230

Heterogeneity in the polyclonal T cell response to birch pollen allergens. PMID: 9363909

Interactions among serum vitamin D binding protein, monomeric actin, profilin, and profilactin. PMID: 2910852

Localization of actobindin, profilin I, profilin II, and phosphatidylinositol-4,5-bisphosphate (PIP2) in Acanthamoeba castellanii. PMID: 9484955

Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells. PMID: 8860230

Profilin is associated with the plasma membrane in microspores and pollen. PMID: 9930655

[Proteins associated with actin: profilin in the trophozoites of Entamoeba histolytica]. PMID: 2136484

Characterization of asparagus allergens: a relevant role of lipid transfer proteins. PMID: 12417890

Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin). PMID: 7751517

Assessing allergen levels in peach and nectarine cultivars. PMID: 17650828

Isolation and localization of a spectrin-like protein from echinoderm sperm. PMID: 1863983

Development and in-house validation of allergen-specific ELISA tests for the quantification of Dau c 1.01, Dau c 1.02 and Dau c 4 in carrot extracts (Daucus carota). PMID: 21046078

N-terminal sequences of high molecular weight allergens from celery tuber. PMID: 10718855

Anaphylaxis to camomile: clinical features and allergen cross-reactivity. PMID: 10998021

Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein). PMID: 7644520

Molecular cloning, over-expression, developmental regulation and immunolocalization of fragminP, a gelsolin-related actin-binding protein from Physarum polycephalum plasmodia. PMID: 9191045

Immunoblot study of IgE binding allergens in celery roots. PMID: 7621060

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-MPXV A42R/Profilin Polyclonal Antibody [PVV21901]
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