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Anti-Human ESRP1 Polyclonal Antibody

Catalog #:   PHK94501 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q6NXG1
Overview

Catalog No.

PHK94501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ESRP1 (Met1-Ser193).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RNA-binding protein 35A, Epithelial splicing regulatory protein 1, ESRP1, RNA-binding motif protein 35A, RBM35A

Purification

Purified by antigen affinity column.

Accession

Q6NXG1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ESRP1 antibody (PHK94501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ESRP1 antibody (PHK94501) at 1 μg/ml.

    Lane 1: RAW264.7

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 76 kDa
    Observed MW: 76 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human skin stained for ESRP1 with PHK94501.
References

Gene expression profiling separates chromophobe renal cell carcinoma from oncocytoma and identifies vesicular transport and cell junction proteins as differentially expressed genes., PMID:17145811

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition., PMID:20711167

Epithelial splicing regulatory protein 1 is a favorable prognostic factor in pancreatic cancer that attenuates pancreatic metastases., PMID:24077287

Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome., PMID:25169209

Epigenetic regulation of ZEB1-RAB25/ESRP1 axis plays a critical role in phenylbutyrate treatment-resistant breast cancer., PMID:26646320

A novel ZEB1/HAS2 positive feedback loop promotes EMT in breast cancer., PMID:28086235

Full-length transcriptome atlas of gallbladder cancer reveals trastuzumab resistance conferred by ERBB2 alternative splicing., PMID:39948369

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Human ESRP1 Polyclonal Antibody [PHK94501]
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