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Anti-NPHS2 Polyclonal Antibody

Catalog #:   PHJ62301 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9NP85
Overview

Catalog No.

PHJ62301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NPHS2 (Met222-Pro372).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

NPHS2, Podocin

Purification

Purified by antigen affinity column.

Accession

Q9NP85

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NPHS2 antibody (PHJ62301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 19 kDa
    Observed MW: 19 kDa
References

Development of a Multiplexed LC-MS/MS Assay for the Quantitation of Podocyte Injury Biomarkers Nephrin, Podocalyxin, and Podocin in Human Urine., PMID:39651829

Immunogenicity, Immunological Memory and Monitoring of Disease Activity Following an Anamnestic Immunization With the 13-Valent Pneumococcal Conjugate Vaccine in Children With Idiopathic Nephrotic Syndrome., PMID:38829802

Feasibility of discontinuing immunosuppression in children with idiopathic nephrotic syndrome., PMID:38270600

Rituximab in idiopathic nephrotic syndrome: still waiting for stronger evidences., PMID:37815432

Successful global anti-B-cell strategy with daratumumab in a patient with post-transplant nephrotic syndrome recurrence unresponsive to immunoadsorption and obinutuzumab., PMID:37226549

Rituximab-associated hypogammaglobulinemia in children with idiopathic nephrotic syndrome: results of an ESPN survey., PMID:37014530

A spectrum of novel anti-vascular endothelial cells autoantibodies in idiopathic nephrotic syndrome patients., PMID:36863601

Comparative Study of Serum IgE Level in Frequent Relapse and Infrequent Relapse Nephrotic Syndrome in Children., PMID:36594295

Collagen IV and Podocyte-Related Gene Variants in Patients with Concurrent IgA Nephropathy and Thin Basement Membrane Nephropathy., PMID:36349777

B cell phenotype, activity, and function in idiopathic nephrotic syndrome., PMID:36316536

Tubular epithelial progenitors are excreted in urine during recovery from severe acute kidney injury and are able to expand and differentiate in vitro., PMID:36285332

Rituximab therapy for childhood onset idiopathic nephrotic syndrome: experience of a Portuguese tertiary center., PMID:36259942

Protocol for an open-label, single-arm, multicentre clinical study to evaluate the efficacy and safety of rituximab in the first episode of paediatric idiopathic nephrotic syndrome., PMID:36223961

Changes in Patterns of Infection Associated with Pediatric Idiopathic Nephrotic Syndrome: A Single-Center Experience in Japan., PMID:36223870

A Novel Mouse Model of Idiopathic Nephrotic Syndrome Induced by Immunization with the Podocyte Protein Crb2., PMID:35985815

Relapse rate in children with nephrotic syndrome during the SARS-CoV-2 pandemic., PMID:35976441

Long-term Rituximab Therapy in Adult Patients with Idiopathic Nephrotic Syndrome., PMID:37929543

Long-Term Efficacy and Safety of Repeated Rituximab to Maintain Remission in Idiopathic Childhood Nephrotic Syndrome: An International Study., PMID:35354600

Seven novel podocyte autoantibodies were identified to diagnosis a new disease subgroup-autoimmune Podocytopathies., PMID:34600127

Huangqi Guizhi Wuwu Decoction attenuates Podocyte cytoskeletal protein damage in IgA nephropathy rats by regulating AT1R/Nephrin/c-Abl pathway., PMID:34339916

A critical re-analysis of cases of post-transplantation recurrence in genetic nephrotic syndrome., PMID:34031708

FOXO3a accumulation and activation accelerate oxidative stress-induced podocyte injury., PMID:32786113

A partial response to abatacept in a patient with steroid resistant focal segmental glomerulosclerosis., PMID:32779421

Idiopathic nephrotic syndrome and serum permeability factors: a molecular jigsaw puzzle., PMID:31848752

Urinary podocyte mRNA is a potent biomarker of anti-neutrophil cytoplasmic antibody-associated glomerulonephritis., PMID:31768862

CD80 expression and infiltrating regulatory T cells in idiopathic nephrotic syndrome of childhood., PMID:31513327

Urokinase-type plasminogen activator contributes to amiloride-sensitive sodium retention in nephrotic range glomerular proteinuria in mice., PMID:31423748

Plasmonic gold nanoparticles as multifaceted probe for tissue imaging., PMID:30766990

Mutational spectrum and novel candidate genes in Chinese children with sporadic steroid-resistant nephrotic syndrome., PMID:30712057

Global polysome analysis of normal and injured podocytes., PMID:30379099

Auto-immune Thyroiditis in an Infant Masquerading as Congenital Nephrotic Syndrome., PMID:30128632

Mesenchymal stem cells prevent podocyte injury in lupus-prone B6.MRL-Faslpr mice via polarizing macrophage into an anti-inflammatory phenotype., PMID:29982691

Adverse events linked with the use of chimeric and humanized anti-CD20 antibodies in children with idiopathic nephrotic syndrome., PMID:29436729

Confocal super-resolution imaging of the glomerular filtration barrier enabled by tissue expansion., PMID:29241621

Nephrotic syndrome in infants and children: pathophysiology and management., PMID:28914167

Down-regulation of PAX2 promotes in vitro differentiation of podocytes from human CD34+ cells., PMID:28852936

Retinoic acid improves nephrotoxic serum-induced glomerulonephritis through activation of podocyte retinoic acid receptor α., PMID:28756872

Abdominal distention and continuous feeding intolerance after intraperitoneal kidney transplant: Answers., PMID:28589211

Randomised controlled trial comparing ofatumumab to rituximab in children with steroid-dependent and calcineurin inhibitor-dependent idiopathic nephrotic syndrome: study protocol., PMID:28314744

TdT-positive Infiltrate in Inflamed Pediatric Kidney: A Potential Diagnostic Pitfall., PMID:28248816

The Effect of Cortex/Medulla Proportions on Molecular Diagnoses in Kidney Transplant Biopsies: Rejection and Injury Can Be Assessed in Medulla., PMID:28226404

Construction of a viral T2A-peptide based knock-in mouse model for enhanced Cre recombinase activity and fluorescent labeling of podocytes., PMID:28187984

Valsartan inhibits amylin-induced podocyte damage., PMID:27102209

Partial podocyte replenishment in experimental FSGS derives from nonpodocyte sources., PMID:27076646

Vascular Endothelial Growth Factor and Podocyte Protection in Chronic Hypoxia: Effects of Endothelin-A Receptor Antagonism., PMID:26930122

[Use of rituximab in primary and secondary glomerulonephritis]., PMID:26845209

Anti-CD20 Antibodies for Idiopathic Nephrotic Syndrome in Children., PMID:26585985

B Cell Reconstitution after Rituximab Treatment in Idiopathic Nephrotic Syndrome., PMID:26567244

Cyclophosphamide and rituximab in frequently relapsing/steroid-dependent nephrotic syndrome., PMID:26525199

Low-dose ofatumumab for rituximab-resistant nephrotic syndrome., PMID:26376698

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-NPHS2 Polyclonal Antibody [PHJ62301]
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