Catalog No.
EVV16001
Expression system
Insect Cells
Species
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Protein length
Met1-Leu183
Predicted molecular weight
20.94 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q65194
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Inner membrane protein p54, pE183L, Ba71V-126, E183L
Epitope mapping of African swine fever virus (ASFV) structural protein, p54. PMID: 32004574
HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies. PMID: 35612629
Lactobacillus plantarum surface-displayed ASFV (p54) with porcine IL-21 generally stimulates protective immune responses in mice. PMID: 34383171
An extensive evaluation of codon usage pattern and bias of structural proteins p30, p54 and, p72 of the African swine fever virus (ASFV). PMID: 34901328
Development of a novel real-time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam. PMID: 34388311
Roles of African Swine Fever Virus Structural Proteins in Viral Infection. PMID: 29978065
Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes. PMID: 35498728
African swine fever virus: A raised global upsurge and a continuous threaten to pig husbandry. PMID: 35526679
Development and characterization of monoclonal antibodies against the N-terminal domain of African swine fever virus structural protein, p54. PMID: 33737177
Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus. PMID: 36366433
Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay. PMID: 35698809
Computational analysis of immunogenic epitopes in the p30 and p54 proteins of African swine fever virus. PMID: 36148815
Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine. PMID: 32915442
Identification of a Linear B Cell Epitope on p54 of African Swine Fever Virus Using Nanobodies as a Novel Tool. PMID: 37191526
Characterization of Anti-p54 Monoclonal Antibodies and Their Potential Use for African Swine Fever Virus Diagnosis. PMID: 33562314
The structural protein p54 is essential for African swine fever virus viability. PMID: 8725112
A Novel Linear B-Cell Epitope on the P54 Protein of African Swine Fever Virus Identified Using Monoclonal Antibodies. PMID: 37112846
Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA. PMID: 34638027
ASFV proteins presented at the surface of T7 phages induce strong antibody responses in mice. PMID: 36965632
ASFV in Tanzania: asymptomatic pigs harbor virus of molecular similarity to Georgia 2007. PMID: 23398669
Characterization and molecular basis of heterogeneity of the African swine fever virus envelope protein p54. PMID: 7933107
The African swine fever virus dynein-binding protein p54 induces infected cell apoptosis. PMID: 15225638
Indirect ELISA Using Multi-Antigenic Dominants of p30, p54 and p72 Recombinant Proteins to Detect Antibodies against African Swine Fever Virus in Pigs. PMID: 36560663
Molecular profile of African swine fever virus (ASFV) circulating in Vietnam during 2019-2020 outbreaks. PMID: 33454861
Molecular characterization of African Swine fever viruses in Burkina Faso, Mali, and Senegal 1989-2016: Genetic diversity of ASFV in West Africa. PMID: 34323385
Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide. PMID: 34715492
Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes. PMID: 19009341
Rapid and ultra-sensitive detection of African swine fever virus antibody on site using QDM based-ASFV immunosensor (QAIS). PMID: 34815032
Screening and identification of the dominant antigens of the African swine fever virus. PMID: 37215478
African swine fever virus structural protein p54 is essential for the recruitment of envelope precursors to assembly sites. PMID: 15047843
Multiple variants of African swine fever virus circulating in Vietnam. PMID: 35190886
Structural models of DYNLL1 with interacting partners: African swine fever virus protein p54 and postsynaptic scaffolding protein gephyrin. PMID: 21094642
Expression and Immunogenicity of Recombinant African Swine Fever Virus Proteins Using the Semliki Forest Virus. PMID: 35615248
Differential antibody responses in sows and finishing pigs naturally infected with African swine fever virus under field conditions. PMID: 34799123
Symptomatic and asymptomatic cases of African swine fever in Tanzania. PMID: 31325213
Splenic-targeting biomimetic nanovaccine for elevating protective immunity against virus infection. PMID: 36463277
Molecular Characterization of African Swine Fever Virus From 2019-2020 Outbreaks in Guangxi Province, Southern China. PMID: 35782548
Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum. PMID: 34454001
African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein. PMID: 11559815
Rapid and visual detection of African swine fever virus antibody by using fluorescent immunochromatography test strip. PMID: 32887174
Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera. PMID: 16490226
Development of optimized protocol for culturing African swine fever virus field isolates in MA104 cells. PMID: 36211218
Flow cytometry-based multiplexing antibody detection for diagnosis of African swine fever virus. PMID: 36038241
Development of an ELISA Method to Differentiate Animals Infected with Wild-Type African Swine Fever Viruses and Attenuated HLJ/18-7GD Vaccine Candidate. PMID: 36016353
Comparison of mucosal immune responses to African swine fever virus antigens intranasally delivered with two different viral vectors. PMID: 35849881
Genetic Characterization of African Swine Fever Virus in Various Outbreaks in Central and Southern Vietnam During 2019-2021. PMID: 36209177
Molecular detection and characterization of African swine fever virus from field outbreaks in domestic pigs, Mizoram, India. PMID: 34743424
Recombinant antigen targets for serodiagnosis of African swine fever. PMID: 19420186
Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection. PMID: 14980493
First detection of African swine fever (ASF) virus genotype X and serogroup 7 in symptomatic pigs in the Democratic Republic of Congo. PMID: 32883295
DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain. PMID: 30696015
Detection and genetic characterization of African swine fever virus (ASFV) in clinically infected pigs in two districts in South Kivu province, Democratic Republic Congo. PMID: 33732937
BacMam immunization partially protects pigs against sublethal challenge with African swine fever virus. PMID: 23428670
Unpicking the Secrets of African Swine Fever Viral Replication Sites. PMID: 33429879
Genetic characterization of African swine fever virus in Cameroon, 2010-2018. PMID: 30929230
Molecular characterization of African swine fever viruses from Burkina Faso, 2018. PMID: 35151326
Orally administered recombinant Lactobacillus expressing African swine fever virus antigens that induced immunity responses. PMID: 36699597
Molecular characterization of African swine fever virus from domestic pigs in northern Tanzania during an outbreak in 2013. PMID: 24996815
Design and construction of African swine fever virus chimeras incorporating foreign viral epitopes. PMID: 10481737
Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011-2013) reveals rapid B602L central variable region evolution. PMID: 29143187
Detection of African swine fever virus in the tissues of asymptomatic pigs in smallholder farming systems along the Kenya-Uganda border: implications for transmission in endemic areas and ASF surveillance in East Africa. PMID: 28721858
Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus. PMID: 36189357
Evaluation of an African swine fever (ASF) vaccine strategy incorporating priming with an alphavirus-expressed antigen followed by boosting with attenuated ASF virus. PMID: 30367292
Evidence for the presence of African swine fever virus in apparently healthy pigs in South-Kivu Province of the Democratic Republic of Congo. PMID: 31902515
Characterization of a Novel African Swine Fever Virus p72 Genotype II from Nigeria. PMID: 37112895
African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. PMID: 33725423
Diversity and emergence of new variants of African swine fever virus Genotype I circulating in domestic pigs in Nigeria (2016-2018). PMID: 36377750
Comparison of the mucosal adjuvanticities of two Toll-like receptor ligands for recombinant adenovirus-delivered African swine fever virus fusion antigens. PMID: 34399310
DNA vaccination partially protects against African swine fever virus lethal challenge in the absence of antibodies. PMID: 23049728
Genetic Characterization of Circulating African Swine Fever Viruses in Nigeria (2007-2015). PMID: 27480888
Re-emergence of genotype I of African swine fever virus in Ivory Coast. PMID: 30548809
For research use only. Not for human or drug use.
